RDB05541 - pKM2L-phHSP701

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pKM2L-phHSP701

Promoter Bank clone, Human 72-kDa heat shock protein (HSP70-1) promoter

Catalog number	RDB05541
Resource name	pKM2L-phHSP701
Alternative name	p1959-17F
Clone info.	PCR amplified human 72-kDa heat shock protein (HSP70-1) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). An HSP70-1 promoter -752 to +164 (+1 as the transcription start site. Fig.2 of Wu, B.J. Proc. Natl. Acad. Sci. USA 83, 629-633, 1986) corresponding to the nucleotide sequence from 1171 to 2086 of DQ451402.1 is cloned. Alternatively, 31814789 to 31815704(NC_000006.12); relatively -725 to +191, where +1 corresponds to 1 nt of NM_005345.6 was cloned.
Comment	PCR cloning, forward primer, 1959 F2: 5' cctcaggcttaaaccaactagggaa 3'; reverse primer, 1959 R2: 5' gaacactggatccgcgagaagag 3' Entire sequence of promoter region has not been confirmed.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human 72-kDa heat shock protein (HSP70-1) genomic DNA
Depositor\|Developer	DNA Bank, \|

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human 72-kDa heat shock protein (HSP70-1)

External Database
Human 72-kDa heat shock protein (HSP70-1)

Reference sequence

Distribution information

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Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05541	pKM2L-phHSP701	DNA solution

Please review the QC test results indicated by check

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References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content	Promoter Collection (Renilla Luciferase) (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14249_A6Gep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB14249_A6Gea.seq
>05541_14249_A6Ge_M13(-40)_A02_02.ab1 1 CGCGGGTCAG CTTGCATGCC TGCAGGTCAT TTAAATAGGG ATAACAGGGT AATGATTAAG 61 ATCTCGAGCT CTAAGCTTCA CATATGCATG CACTAGTGGC CCTCAGGCTT AAACCAACTA 121 GGGAACTTTC CAGTACTTTC CCAAACAAGG ACCTACTGAG CCTTTCAGGT TCACAATCAA 181 TCAGATCCCT ACTGGCTCAC CTAGTCTCCC GACGCCTTCG CTTCAGTTTG GAAACGTCCA 241 GATTACGCAG CCCCAGCGAG TAGGTGGGGG CTCCCTCAAT ATCAAACTGC ACAACCGGGG 301 TCCCCCCACC CCCCACCCCG TCCCTCCCTG CAAATTTGAG ACGGCTCCAA CTCAGTAATC 361 TTTTTCCAAA CTGGCCCATG AGGTCAGAGA CAGTATCTCC ATTGTAACGT GGCCGGGCGG 421 TGTCAACACA AACGCCCCCA CCCTCCCCTG GACGCGCGTA ACCCGCTCCC CGCACCAGCC 481 CCCTGCCCAC AACTGCGCAG GCCCAGCAAG CCCCCACAAT TAAAAGCCCA GCGCCGACCC 541 TTCCTGTCAA TTAGGCGCTG AAGCGCAGGC GGTCAGCATC GCCATGGAGA CCAACACCCT 601 TCCCACCGCC ACTCCCCCTT CCTCTCAGGG TCCCTGTCCC CTCCAGTGAA TCCCAGAAGA 661 CTCTGGAGAG TTCTGAGCAG GGGGCGGCAC TCTGGCCTCT GATTGGTCCA AGGAAGGCTG 721 GGGGGCAGGA CGGGAGGCGA AAACCCTGGA ATATTCCCGA CCTGGCAGCC TCATCGAGCT 781 CGGTGATTGG CTCAGAAGGG AAAAGGCGGG TCTCCGTGAC GACTTATAAA AGCCCAGGGG 841 CAAGCGGTCC GGATAACGGC TAGCCTGAGG AGCTGCTGCG ACAGTCCACT ACCTTTTTTC 901 GAGAGTGACT CCCGTTGTCC CAAAGGCTTC CCAGAGCGAA ACCTGTGCGG CTGCAGCACC 961 GGGCGCGTCG AGTTTCCCGG CGTCCGGGAA AGGACCCGAG CTCTTCTCGC GGGATCCCAG 1021 TGCTCGCCTG TCGACGCGTA GAACTCACCC GGGTTACTGC AGAAGTTGGA TCGTGAGGCA 1081 CTTGGGCAAG CTTAAGCTAT CAAGGGTTAA CCAG //
Primer: phRLR2 Sequence file: RDB14249_A6Geb.seq
>05541_14249_A6Ge_phRLR2_B02_05.ab1 1 ATCCTATCTG ACAGCCCAGT TTCTATTGGT CTCCTTAAAC CTGTCTTGTA ACCTTGATAC 61 TTACCTGCCC AGTGCCTCAC GACCAACTTC TGCAGTACCC GGGTGAATTC TACGCGTCGA 121 CAGGCGAACA CTGGATCCGC GAGAAGAGCT CGGTCCTTCC GGACGCCGGA AACTCGACGC 181 GCCGGTGCCT GCAGCCGCAC AGGTTCGCTC TGGGAAGCCT TGGGACAACG GGAGTCACTC 241 TCGAAAAAGG TAGTGGACTG TCGCAGCAGC TCCTCAGGCT AGCCGTTATC CGGACCGCTT 301 GCCCCTGGGC TTTTATAAGT CGTCACGGAG ACCCGCCTTT TCCCTTCTGA GCCAATCACC 361 GAGCTCGATG AGGCTGCCAG GTCGGGAATA TTCCAGGGTT TTCGCCTCCC GTCCTGCCCC 421 CCAGCCTTCC TTGGACCAAT CAGAGGCCAG AGTGCCGCCC CCTGCTCAGA ACTCTCCAGA 481 GTCTTCTGGG ATTCACTGGA GGGGACAGGG ACCCTGAGAG GAAGGGGGAG TGGCGGTGGG 541 AAGGGTGTTG GTCTCCATGG CGATGCTGAC CGCCTGCGCT TCAGCGCCTA ATTGACAGGA 601 AGGGTCGGCG CTGGGCTTTT AATTGTGGGG GCTTGCTGGG CCTGCGCAGT TGTGGGCAGG 661 GGGCTGGTGC GGGGAGCGGG TTACGCGCGT CCAGGGGAGG GTGGGGGCGT TTGTGTTGAC 721 ACCGCCCGGC CACGTTACAA TGGAGATACT GTCTCTGACC TCATGGGCCA GTTTGGAAAA 781 AGATTACTGA GTTGGAGCCG TCTCAAATTT GCAGGGAGGG ACGGGGTGGG GGGGGGTGGG 841 GGGGGACCCC GGTTGTGCAG TTTTGATATT GGAGGGAGCC CCCCACCCTA CTCGCTGGGG 901 GCTGCGTAAT CTGGGACGTT TCCAAACTGA AAGCGAAAGG CGTCGGGAGA CTAGGTGAGC 961 CAGTAGGGAT CTGATTGATT GTGAACCCTG AAGCTCAGTA GTCCCTTGTT TGGGAAAGTA 1021 CTGCAAAGTT CCCTAGTTTG GTTTAGGCCT GAAGGACCAA CTAGTGCATG CATATGTGAA 1081 AGGCTTAAGA GCTCGGAGAA TTCATTAATT CAA //
Primer: pAxCALNL_F1 Sequence file: RDB14249_A6Gec.seq
>05541_14249_A6Ge_pAxCALNL_F1_C02_08.ab1 1 TCATTATCTT ATCATGTCTG CTCGAAGCGG CCGCTCTAGA ATTACTGCTC GTTCTTCAGC 61 ACGCGCTCCA CGAAGCTCTT GATGTACTTA CCCATTTCAT CTGGAGCGTC CTCCTGGCTG 121 AAGTGGAGGC CCTTCACCTT CACGAACTCG GTGTTAGGGA ACTTCTTAGC TCCCTCGACA 181 ATAGCGTTGG AAAAGAACCC AGGGTCGGAC TCGATGAACA TCTTAGGCAG ATCGTCGCTG 241 GCCCGAAGGT AGGCGTTGTA GTTGCGGACA ATCTGGACGA CGTCGGGCTT GCCTCCCTTA 301 ACGAGAGGGA TCTCGCGAGG CCAGGAGAGG GTAGGCCGTC TAACCTCGCC CTTCTCCTTG 361 AATGGCTCCA GGTAGGCAGC GAACTCCTCA GGCTCCAGTT TCCGCATGAT CTTGCTTGGG 421 AGCATGGTCT CGACGAAGAA GTTATTCTCA AGCACCATTT TCTCGCCCTC TTCGCTCTTG 481 ATCAGGGCGA TATCCTCCTC GATGTCAGGC CACTCGTCCC AGGACTCGAT CACGTCCACG 541 ACACTCTCAG CATGGACGAT GGCCTTGATC TTGTCTTGGT GCTCGTAGGA GTAGTGAAAG 601 GCCAGACAAG CCCCCCAGTC GTGGCCCACA AAGATGATTT TCTTTGGAAG GTTCAGCAGC 661 TCGAACCAAG CGGTGAGGTA CTTGTAGTGA TCCAGGAGGC GATATGAGCC ATTCCCGCTC 721 TTGCCGGACT TACCCATTCC GATCAGATCA GGGATGATGC ATCTAGCCAC GGGCTCGATG 781 TGAGGCACGA CGTGCCTCCA CAGGTAGCTG GAGGCAGCGT TACCATGCAG AAAAATCACG 841 GCGTTCTCGG CGTGCTTCTC GGAATCATAG TAGTTGATGA AGGAGTCCAG CACGTTCATT 901 TGCTTGCAGC GAGCCCACCA CTGAGGCCCA GTGATCATGC GTTTGCGTTG CTCGGGGTCG 961 TACACCTTGG AAAGCCATGG GTGGGCTAGC CCTATAGTGA GTCGGTATTT AAGTACTCTA 1021 GCCCTTAAGA GCCTGGTAGT GGAACTGGGG AGTTGACACT TGTTGAGAAG AAAGGCAAGC 1081 TTGGTAGTTC AGTAAGACCA ATTACG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference	Wu, B.J., Human HSP70 promoter contains at least two distinct regulatory domains. Proc. Natl. Acad. Sci. USA. 83 (3): 629-633 (1986). PMID 3456160.

2022.05.18

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