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pKM2L-phGCG

Promoter Bank clone, Human glucagon promoter

Catalog number RDB05490
Resource name pKM2L-phGCG
Alternative name p1898-1F
Clone info. PCR amplified human glucagon promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 162153856 to 162152194(NC_000002.12); relatively -1452 to +211, where +1 corresponds to 1 nt of NM_002054.4.
Comment PCR cloning, forward primer, 1898F-SpeI: 5' gactactagtctagaccgacctaaggcttgacacc 3'; reverse primer, 1898R-SalI: 5' gactgtcgacagagagcaagccctctttgggaac 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human glucagon genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human glucagon

          Reference sequence
            AC007750

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05490 pKM2L-phGCG DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB05490_A6A7p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB05490_A6A7a.seq check
          >05490_05490_A6A7_2_M13(-40)_B10_04.ab1
              1 CGGGGGTCAG CTTGCATGCC TGCAGGTCAT TTAATAGGGA TAACAGGGTA ATGATTAAGA
             61 TCTCGAGCTC TAAGCTTCAC ATATGCATGC ACTAGTCTAG ACCGACCTAA GGCTTGACAC
            121 CTTTCTCTGC TCTTTCACCC CCTCTGCTGG TAGAGTGCCA AGAAGCAGGT CTCAGATCAA
            181 GACACCAGCA GTACCAATGA GGAGATAAAG TGAGGCAGAT ATTTTATGCA GCTGTGCTTC
            241 TCAAATTTTA ATATGAACAC AGATTTCCTT AGGATTTTGT TAAAATACAG ATTCTGATAG
            301 GCACGAGATT CTGCATTTCT AGCAAGCTCC CAGATGATGC TGGTCCTTAG ACCACACTTT
            361 GCAAAGCAAG GCTTTCTAGC ACCCAATAAT CACCAGCACA AAAACCCCTC AATGATGTCT
            421 TCCAGTCTGA CTTGAAAGTG ATCTCTGTGG CCTTCAAGTA GCTCTACATT CAAACGTTGG
            481 TCTCATGCAC AAATTGAACA GTTAAGTTCA TTTGGAATAT TTCTCCAGTT GCCTCAATGA
            541 TTTACCTCCA TACAAGTCAT CAGTGACATC ATGCGGTTGG TTATATTAGA ATATTGGAAT
            601 CTAGTTAGGA AATGTTGTGA TGAGTTTGCA ATATGGAATA AAATTTTTAT TTTAGAAAAG
            661 ACATTTACCA ACCCCTCTCA TTTTACAAAT AAGGAGGCAG AGACACAAAG AGAATGCGTG
            721 ACTTGACCAA GGTCACACAG CTGGTCAATA ACAGCAAAAC TAAAATCAAA TTATGCCAAC
            781 ACTACTTCTT ATGCTGTCTT TCCAGATTAT TTACCATTTT TCTATCTCTC TACAGTGACC
            841 AGAATCATTT CTGCTAAATG TCACAGATCA TTAAAGCCTG TGTGTCCAGT CACAAAACTC
            901 AGGAAACGTG AAAATATGCA TCTCATCTCA ACAGTTTTTC CTCATATCTC ATTCTTTTGT
            961 AAACTTAGTA CCCCCACTCT CTTATCAGTA AAAATTTAGA TTTTTTAAAT ATATATTAGA
           1021 ATGGAAAAAA ATACCCAGAT CATTTCCTGG CTAGCATTCA GCTATTCTTG GCATGCTTTT
          1081 AATTCCATCG A
          //
          Primer: phRLR2
          Sequence file: RDB05490_A6A7b.seq check
          >05490_05490_A6A7_2_phRLR2_E10_13.ab1
              1 ATCCAGACTC GAAGCCCAGT TTCTATTGGT CTCCTTAACC TGTCTTGTAA CCTTGATACT
             61 TACCTGCCCA GTGCCTCACG ACCAACTTCT GCAGTACCCG GGTGAATTCT ACGCGTCGAC
            121 AGAGAGCAAG CCCTCTTTGG GAACTTTTGA TGTGCTCTGT GTCCTAAGCT CTGTACTTCT
            181 GCACCAAGGC GCACTGCTTT TATATACTCT CCAGCTCTGT TTAGCCTCAC AGATATTACG
            241 CTGACAATAT AAATTTCTCA TCTGTAAATA ATGAGTTTTT GTTACAAATG ACTTCCCTCT
            301 CCCACTCACT TTGCTCATCT GCATTCTATG CTTATATTGG GTGAGAGACC TTAGAGACCT
            361 GGAAACTTTT TATGCACTCT TGTATCTTTT TTTTTTTTTG GTTGGGAATG GAGAGAGCAG
            421 CTTGGAAATC CACCTTCTAC TTTCAATCAC GCATGAAAAT CACTTAAGTT CTCTAAAGTG
            481 AATTTTGACG TTGAAGACAA AAGACCTTGA AATTACGTAG CCCATGGCCA TCCCTGTTTT
            541 GTGCATGTCC TCATTTGAGG TTCTTACAAG TCTTTGGTTT GCTACCTAGC CATGATGAAT
            601 TTACCTGAAT ATAACAATCA CTTCAGTCAT TCATTTATCT AAATAATGAT CATTCATTCA
            661 ACACATATGT ATTAAAAATC CTCTCTTCCT CACAACGTGG AGTTTATTTA TGTTATGTTT
            721 GTGGAAAATT TAAGGATATT GATTAAACCC CTATGTGTTC TGTTTTAATA AATGGAATTC
            781 AGGCAGAAAG GATGGAGCAA AATGAAGATT AAACATCCAA GATAGCTGAT GCTAACAGGA
            841 AATTATCTGG TATTTTTTTC CTTCTAATAT ATATTTAAAA TCTAATTTTA CTGATAAGAG
            901 AGTGGGGTAC TAAGTTACAA AAGAATGAGA TATGAGGAAA ACTGTTTGAG ATGAGATGCA
            961 TATTTTTCAC GTTTCCCTGA GTTTTGTGAC TGGACACACA GGCTTTAAAT GATCTGTGAC
           1021 ATTTAGCCAG AAATTGAATC CTGGTTCACT GTAGAGCATA GCAACAATGC TCAATAAGTC
          1081 TGGCAAAAGG ACCAGGCATT AAG
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB05490_A6A7c.seq check
          >05490_05490_A6A7_2_pAxCALNL_F1_H10_22.ab1
              1 TAGGTATCTT ATCATGTCTG CTCGAGCGGC CGCTCTAGAA TTACTGCTCG TTCTTCAGCA
             61 CGCGCTCCAC GAAGCTCTTG ATGTACTTAC CCATTTCATC TGGAGCGTCC TCCTGGCTGA
            121 AGTGGAGGCC CTTCACCTTC ACGAACTCGG TGTTAGGGAA CTTCTTAGCT CCCTCGACAA
            181 TAGCGTTGGA AAAGAACCCA GGGTCGGACT CGATGAACAT CTTAGGCAGA TCGTCGCTGG
            241 CCCGAAGGTA GGCGTTGTAG TTGCGGACAA TCTGGACGAC GTCGGGCTTG CCTCCCTTAA
            301 CGAGAGGGAT CTCGCGAGGC CAGGAGAGGG TAGGCCGTCT AACCTCGCCC TTCTCCTTGA
            361 ATGGCTCCAG GTAGGCAGCG AACTCCTCAG GCTCCAGTTT CCGCATGATC TTGCTTGGGA
            421 GCATGGTCTC GACGAAGAAG TTATTCTCAA GCACCATTTT CTCGCCCTCT TCGCTCTTGA
            481 TCAGGGCGAT ATCCTCCTCG ATGTCAGGCC ACTCGTCCCA GGACTCGATC ACGTCCACGA
            541 CACTCTCAGC ATGGACGATG GCCTTGATCT TGTCTTGGTG CTCGTAGGAG TAGTGAAAGG
            601 CCAGACAAGC CCCCCAGTCG TGGCCCACAA AGATGATTTT CTTTGGAAGG TTCAGCAGCT
            661 CGAACCAAGC GGTGAGGTAC TTGTAGTGAT CCAGGAGGCG ATATGAGCCA TTCCCGCTCT
            721 TGCCGGACTT ACCCATTCCG ATCAGATCAG GGATGATGCA TCTAGCCACG GGCTCGATGT
            781 GAGGCACGAC GTGCCTCCAC AGGTAGCTGG AGGCAGCGTT ACCATGCAGA AAAAATCACG
            841 GCGTTCTCGG CGTGCTTCTC GGAATCATAG TAGTTGATGA AGGAGTCCAG CACGTTCATT
            901 TGCTTGCAGC GAGCCCACCC ACTGAGGCCC AGTTGATCAT GCGTTTGCGT TTGCTCGGGG
            961 GTTCGGTACA CCTTTGCAAG CCCATGGTGG CCTTAGCTAT AGTGAGTCGT AATAGGTACT
          1021 CTAGCCCTTT AGGAGCTGTA ATGAACTTGG TAGTGACACC T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl