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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKM2L-phMMP1

Promoter Bank clone, Human collagenase-1 (MMP-1) promoter

Catalog number RDB05478
Resource name pKM2L-phMMP1
Alternative name p1886-2F
Clone info. PCR amplified human collagenase-1 (MMP-1) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 102800490 to 102798158 (NC_000011.10); relatively -2255 to +78, where +1 corresponds to 1nt of NM_002421.3.
Comment PCR cloning, forward primer, 1886 F1: 5' gtttccaatccacgtcagcaact 3'; reverse primer, F2PB0014R: 5' tatcccagctaggaagctccctctg 3' Known differences with NC_000011.10: A to G substitution at 102,798,479 and T to C substitution at 102,800,447. Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human collagenase-1 (MMP-1) genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human collagenase-1 (MMP-1)
          Reference sequence
            U78045, AF023338, AF007878

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05478 pKM2L-phMMP1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB05478z.seq

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Renilla Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17373_A9Fap1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB17373_A9Faa.seq check
          1
          Primer: phRLR2
          Sequence file: RDB17373_A9Fab.seq check
          >05478_17373_A9Fa_1_phRLR2_F07_06_ABI08.ab1
              1 ATTCTTGGTC TCGAACAGCC CAGTTTCTAT TGGTCTCCTT AAACCTGTCT TGTAACCTTG
             61 ATACTTACCT GCCCAGTGCC TCACGACCAA CTTCTGCAGT ACCCGGGTGA ATTCTACGCG
            121 TCGACAGGCT ATCCCAGCTA GGAAGCTCCC TCTGTATATA TAGAGTCCTT GCCCTTCCAG
            181 AAAGCCAGAG GCTGTCTGAC TCATGCTTTA TAACATCCTC TTGATTAGCT ATGAATAGAC
            241 TAGGCAATCA TTAGAAATGG TGACTCCTAG CAGATTATTT GGAATCACTT GGTGTTGCAA
            301 TGTCATGATT AACTTCAAAC AAGATGTGTG CGAAGGAGAC ACACTCTGCC ATGTAAACAG
            361 TGGTTTTCTC CAATTCATTT CTTTCCTATT TGAGAAAGGT TCTTGTTATA TACACAGGTC
            421 AAAGAGTACT CCATGGTCTT TTTGAAAAAG GCTGGTTCTG ATGGTCATAA AGTGCTACAG
            481 GTTTCTCCAC ACACCTTGCT CCCAGGGCAG AGGGTGGAAT TACTAACACT GCGCACCTGA
            541 TGGCTGTTCG GCACCTGTAC TGACTGAAGT TCAGCTCCCC CAGCACTCAC TTTACGGTGG
            601 CTCTTCGGGG TTCTCTGAGG TTCCCTTCTG CCTTTGTTAA GCTGCCTGGT ACCCTATTGC
            661 GATAGCACCA TGGCTTCCCT TAATCTTTAC TCAGAGCCAG GCATGGTGGC TCAAGCCTAT
            721 AATCCCAGAA TTTCAGGAGG CCGAGGCAGG GGAATCACTT GAGCCCAGGA GTTCAAGATC
            781 AGCCTGGGCA ACATGGTGAG ACCCCATCTC TGTAAAAAAC AAACAAACAA ACAAACATTT
            841 TTTAAAGTTA GCCAAGCATG GAGTCATGCA CCTGTAGTCC TAGTCACTGA AGAGGCTGAG
            901 TGGGAGGATC ACTTGGGCCT GGGGAGGTTG AGGCTGCATT GAGCTGTGAT TGTGCCGCTG
            961 CACTCCAGCC TAGCACAGAG AGGGACCCTG TCTAAATAAA TTTGAAAAAA ATTTACTCAG
           1021 GCCTAGGATT AAGAGCCTTT ACCTGAAGAA GACCCCCTCA TCCACAAGCC GGAGTATGAG
           1081 ATACCTCCCC CACCCCCTTT GCCCATCATT ATCATGGGAC AAATAGGGAA TCTTCCTGGA
           1141 ACTGTTCTGA AATGTAACCG ATGAATGAAA AGGGCTGGAA AGGAACTGAA CGGTAAACTC
          1201 TGGAGACTCG TAGAT
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB17373_A9Fac.seq check
          >05478_17373_A9Fa_1_pAxCALNL_F1_G07_07_ABI08.ab1
              1 CACCAGGTAT CTTATCATGT CTGCTCGAAG CGGCCGCTCT AGAATTACTG CTCGTTCTTC
             61 AGCACGCGCT CCACGAAGCT CTTGATGTAC TTACCCATTT CATCTGGAGC GTCCTCCTGG
            121 CTGAAGTGGA GGCCCTTCAC CTTCACGAAC TCGGTGTTAG GGAACTTCTT AGCTCCCTCG
            181 ACAATAGCGT TGGAAAAGAA CCCAGGGTCG GACTCGATGA ACATCTTAGG CAGATCGTCG
            241 CTGGCCCGAA GGTAGGCGTT GTAGTTGCGG ACAATCTGGA CGACGTCGGG CTTGCCTCCC
            301 TTAACGAGAG GGATCTCGCG AGGCCAGGAG AGGGTAGGCC GTCTAACCTC GCCCTTCTCC
            361 TTGAATGGCT CCAGGTAGGC AGCGAACTCC TCAGGCTCCA GTTTCCGCAT GATCTTGCTT
            421 GGGAGCATGG TCTCGACGAA GAAGTTATTC TCAAGCACCA TTTTCTCGCC CTCTTCGCTC
            481 TTGATCAGGG CGATATCCTC CTCGATGTCA GGCCACTCGT CCCAGGACTC GATCACGTCC
            541 ACGACACTCT CAGCATGGAC GATGGCCTTG ATCTTGTCTT GGTGCTCGTA GGAGTAGTGA
            601 AAGGCCAGAC AAGCCCCCCA GTCGTGGCCC ACAAAGATGA TTTTCTTTGG AAGGTTCAGC
            661 AGCTCGAACC AAGCGGTGAG GTACTTGTAG TGATCCAGGA GGCGATATGA GCCATTCCCG
            721 CTCTTGCCGG ACTTACCCAT TCCGATCAGA TCAGGGATGA TGCATCTAGC CACGGGCTCG
            781 ATGTGAGGCA CGACGTGCCT CCACAGGTAG CTGGAGGCAG CGTTACCATG CAGAAAAATC
            841 ACGGCGTTCT CGGCGTGCTT CTCGGAATCA TAGTAGTTGA TGAAGGAGTC CAGCACGTTC
            901 ATTTGCTTGC AGCGAGCCCA CCACTGAGGC CCAGTGATCA TGCGTTTGCG TTGCTCGGGG
            961 TCGTACACCT TGGAAGCCAT GGTGGCTAGC CTATAGTGAG TCGTATTAAG TACTCTAGCC
           1021 CTTAAGAGCT GTAATTGAAC TGGGAGTGGA CACCTGTGGA GGAGAAAGGC AAAGTGGATG
           1081 TCAGTTAAGA TAATAGGTGC TTATCAGAAA CGCAGAGTCT CTCTGGTTTC GACAGGCCCA
          1141 GTTTCTATTG GATCTCGTTT AAACTGTGTC TTTGGTTAAC CT
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl