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pKM2L-phBMP6

Promoter Bank clone, Human bone morphogenetic protein 6 (BMP6) promoter

Catalog number RDB05474
Resource name pKM2L-phBMP6
Alternative name p1882-5F
Clone info. The Human bone morphogenetic protein 6 (BMP6) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 7725636 to 7726955(NC_000006.12); relatively -463 to +857, where +1 corresponds to 1 nt of NM_001718.5; relatively -1142 to +178, where +1 corresponds to 1 nt of NM_001718.4.
Comment PCR cloning forward primer, B2PB0010F: 5' ctgccgtggaaagccacgtgtcc 3'; reverse primer, B2PB0010R: 5' ccccgcccggccgggctgccc 3' Reference: Fig.2 of Tamada, H., Biochim. Biophys. Acta 1395 (3): 247-251 (1998). Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human bone morphogenetic protein 6 genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human bone morphogenetic protein 6
          Reference sequence
            AF083030
           

          Distribution information

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          Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05474 pKM2L-phBMP6 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pKM2L-phBMP6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05474).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

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          Featured content

          Featured content Promoter Collection (Renilla Luciferase) (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14631_A7Bsp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB14631_A7Bsa.seq check
          >05474_14631_A7Bs_M13(-40)_B08_02_ABI08.ab1
              1 CCGGCCGTCC AGCTTGCATG CCTGCAGGTC ATTTAATAGG GATAACAGGG TAATGATTAA
             61 GATCTCGAGC TCTAAGCTTC ACATATGCAT GCACTAGTGG CCTGCCGTGG AAAGCCACGT
            121 GTCCGCTAAA CGCCTACATT ACATCAATAA GAGAAACTAA GGAAGGGATG GAAAAGCTAT
            181 CATTTTTCTT TTCTTCCTCA GTTCTGGCAG TATAGTGATT TAATCGCTGT TATTATTTTT
            241 ATTTAGTTAA AGCATCAACT TAGGCCTTTT AAATGATGGT AAAAGAGAAA GCTTCAGATC
            301 GGGGTATTGG TCAGATCATT CTTACTAGCG ATGACCTCTT CGCCTTTGGC ACCTCGCGGC
            361 TCTTCGCATG CCTCCAAGAA TTCCCGGGTC TTGAAACCTA CGCGCGGCGG AGGCGACGCC
            421 TCTCGGGAGA GGGAGGGTGA AAGAAGGGGC GGGGCTATTT AAATAGCCCG CGAGCTTCGC
            481 GTCCCCATTG GCTGCCAGCC TGTCGGTTAC CGCGGGGCGG GGAGTCCCGG GGCTGGAGTG
            541 AGGTACCTGA GCCGGGCGCG CAGCAGAAAG CCCACGTTGG CCGCTGCGGG GAGCGCGGCG
            601 TGGAGAGGCG GGAGACCGAA TTCCGGCGCC GCCGCCGCGG CCGCTGCTCG GTGCACTAGC
            661 CCCCTTCCTT CCCATCCTTT CTGCGAGCGG GTTTGCTGGG CAGCCGGGCG ACCGCCGAAC
            721 GGAAAGCAAG ATCCTCGCCG CCTCCGCTTG AGGAGGCGTG CGGCGCGCGG AGATTTTGAG
            781 TGGGAGCGAC GGTGCCCGAG AGCTCAGGGG CGCGGAGTTC GTGAGCGAGA AGGAAGTTAA
            841 ACCTCGCGGA ATAGACTGGC ATTTCGGGAC GCCTTGTCGC CGCAGCCTGG GCCCTGCAAC
            901 GGGGTAAACT TCATGGTGGC CCTGCGATCT GGGGAGGGGC GTGCGCCACG GTGATGCCGG
            961 GCATCTCCCG CAGCTCGTGA GCGGCCCCGC TCCCCGCTGC CCCGGGTCCC ACTCAGTCGC
           1021 CAAGGGAGAG CGCGGGGCAG CGCACGTGTG CGGCCAGCGA GGCCCAGAGT GACCGCGCCG
           1081 CGACTCGCAG GAGCCAAGGG CGCAAGGCTG CAATGGCAGC CCGGACCTCA GACGGCACCT
           1141 GCCTGGAACC GCCGCGTCTT CTAGAGACCT GCGCGAGAGG CCTGTGTGAA GCTCCCTTCC
          1201 TTCCCTCTCC AAGGCGGGAT CTCTCTTGGG GAGATCTC
          //
          Primer: phRLR2
          Sequence file: RDB14631_A7Bsb.seq check
          >05474_14631_A7Bs_phRLR2_E07_05_ABI08.ab1
              1 AACTTTTTCC TGTCTGACAA GCCCAGTTTC TATTGGTCTC CTTAAACCTG TCTTGTAACC
             61 TTGATACTTA CCTGCCCAGT GCCTCACGAC CAACTTCTGC AGTACCCGGG TGAATTCTAC
            121 GCGTCGACAG GCCCCCGCCC GGCCGGGCTG CCCCCGCGGA TCCCGCGAGG CGTGGAGCGG
            181 CGGAGCGAGG CCGGAGCGCG GCCGCTGTGG CCCTTGGCGT GAGCAGTCCC CGCCACCTCT
            241 CGGCGGGCTC GCTTCCCCTT CCTGGCCCTC AGTCCTTATC TCTCATGGTC GTCCGGGGCG
            301 CCCCCAGTTG CCCAGGCTAG AGAGGTGGCG AAGGGGCGAT CACCAGGAGA ACCGCTTGGA
            361 GGGGAGGAAG GGGAGCCTCA CAGCCTCGCG CAGGTCTCTA GAGGCGCGCG GTCCAGGCCA
            421 GGTGCGTCTG AGTCCGGGCT GCACTGCAGC CTGCGCCCTG GCTCCTGCGA GTCGCGGCGC
            481 GGTCACTCTG GGCCTCGCTG GCCGCACACG TGCGCTGCCC CGCGCTCTCC CTGGCGACTG
            541 AGTGGGACCC GGGGCAGCGG GGAGCGGGGC CGCTCACGAG CTGCGGGAGA TGCCCGGCAT
            601 CACCGTGGCG CACGCCCCTC CCCAGATCGC AGGGCCACCA TGAAGTTTAC CCCGTTGCAG
            661 GGCCCAGGCT GCGGCGACAA GGCGTCCCGA AATGCCAGTC TATTCCGCGA GGTTTAACTT
            721 CCTTCTCGCT CACGAACTCC GCGCCCCTGA GCTCTCGGGC ACCGTCGCTC CCACTCAAAA
            781 TCTCCGCGCG CCGCACGCCT CCTCAAGCGG AGGCGGCGAG GATCTTGCTT TCCGTTCGGC
            841 GGTCGCCCGG CTGCCCAGCA AACCCGCTCG CAGAAAGGAT GGGAAGGAAG GGGGCTAGTG
            901 CACCGAGCAG CGGCCGCGGC GGCGGCGCCG GAATTCGGTC TCCCGCCTCT CCACGCCGCG
            961 CTCCCCGCAG CGGCCAACGT GGGCTTTCTG CTGCGCGCCC GGCTCAGTAC CTCACTCCAG
           1021 CCCCGGGACT CCCCGGCCCG CGGTAACCGA CAGGCTGGCA GCCAATGGGG ACGCGAAGCT
           1081 CGCGGGCTAT TTAAATAGCC CCGCCCCTTC TTTCACCCTC CCTCTCCGAG GAGCGTCGCC
           1141 TCCGCCGCCG CGTAGTTTCA GGACCCGGAT TCTTGGAGCA TGCAGAGCCC CAGTGCAAGC
          1201 GAAGTCATCG CTAGTAGAAT GATCTGGACC CATTACCCGA ATCTCTGGGA GCCTTTTC
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB14631_A7Bsc.seq check
          >05474_14631_A7Bs_pAxCALNL_F1_F07_06_ABI08.ab1
              1 AATAAATGTA TCTTATCATG TCTGCTCGAA GCGGCCGCTC TAGAATTACT GCTCGTTCTT
             61 CAGCACGCGC TCCACGAAGC TCTTGATGTA CTTACCCATT TCATCTGGAG CGTCCTCCTG
            121 GCTGAAGTGG AGGCCCTTCA CCTTCACGAA CTCGGTGTTA GGGAACTTCT TAGCTCCCTC
            181 GACAATAGCG TTGGAAAAGA ACCCAGGGTC GGACTCGATG AACATCTTAG GCAGATCGTC
            241 GCTGGCCCGA AGGTAGGCGT TGTAGTTGCG GACAATCTGG ACGACGTCGG GCTTGCCTCC
            301 CTTAACGAGA GGGATCTCGC GAGGCCAGGA GAGGGTAGGC CGTCTAACCT CGCCCTTCTC
            361 CTTGAATGGC TCCAGGTAGG CAGCGAACTC CTCAGGCTCC AGTTTCCGCA TGATCTTGCT
            421 TGGGAGCATG GTCTCGACGA AGAAGTTATT CTCAAGCACC ATTTTCTCGC CCTCTTCGCT
            481 CTTGATCAGG GCGATATCCT CCTCGATGTC AGGCCACTCG TCCCAGGACT CGATCACGTC
            541 CACGACACTC TCAGCATGGA CGATGGCCTT GATCTTGTCT TGGTGCTCGT AGGAGTAGTG
            601 AAAGGCCAGA CAAGCCCCCC AGTCGTGGCC CACAAAGATG ATTTTCTTTG GAAGGTTCAG
            661 CAGCTCGAAC CAAGCGGTGA GGTACTTGTA GTGATCCAGG AGGCGATATG AGCCATTCCC
            721 GCTCTTGCCG GACTTACCCA TTCCGATCAG ATCAGGGATG ATGCATCTAG CCACGGGCTC
            781 GATGTGAGGC ACGACGTGCC TCCACAGGTA GCTGGAGGCA GCGTTACCAT GCAGAAAAAT
            841 CACGGCGTTC TCGGCGTGCT TCTCGGAATC ATAGTAGTTG ATGAAGGAGT CCAGCACGTT
            901 CATTTGCTTG CAGCGAGCCC ACCACTGAGC CCAGTGATCA TGCGTTTGCG TTGCTCGGGG
            961 TCGTACACCT TGGAAGCCAT GGTGGCTAGC CTATAGTGAG TCGTATTAAG TACTCTAGCC
           1021 TTAAGAGCTG TAATTGAACT GGGAGTGGAC ACCTGTGGAG AGAAGGCAAA GTGATGTCAG
           1081 TAGACCATAG GTGCCTATCA GAAACGCAGA GTCTTCTCTG TTCTCGACAA GCCCAGGTTT
           1141 CTATTGTCTT CTTAACTGTC TGTACCTTGA TACTACTGCC AAGTGCTCAC GACCACCTTC
          1201 TGCAGTACTG TGATCTAACG CGTCGACAGG CCCCGCGCCC GGTCCGGGTG TC
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          reference Tamada, H., Molecular cloning and analysis of the 5'-flanking region of the human bone morphogenetic protein-6 (BMP-6). Biochim. Biophys. Acta 1395 (3): 247-251 (1998). PMID 9512655.
          user_report Ohta, K., Role of hepcidin upregulation and proteolytic cleavage of ferroportin 1 in hepatitis C virus-induced iron accumulation. PLoS Pathog. 19 (8): e1011591 (2023). PMID 37585449. [link to RRC of NBRP]

          2024.04.04

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