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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAxEFwtit2

Dual cassette vector to generate recombinant adenovirus containing EF1 alpha promoter

Catalog number RDB05215
Resource name pAxEFwtit2
Clone info. A dual cassette for constructing recombinant adenovirus containing EF1alpha promoter. Csp45I and PacI cab be used to generate recombinant adenovirus by transfection. Conversion to recombinant adenovirus was confirmed with HEK293 cell (Nov, 2005).
Comment The pAxEFwtit2 contains an expression cassette, consisting of the promoter of a gene for elongation factor 1alpha (EF-1alpha), a unique SwaI site and a polyadenylation signal from SV40 (S polyA), and was cloned into the SwaI site of pAxcwit2 (RDB 5212). The pAxEFwtit2 cosmid, digested with Csp45I or PacI, can generate rAd upon transfection of HEK293 cells and can also be used to generate rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996].
Vector backbone Charomid 9-11 (Cosmid, use packaging extracts for transformation)
Size of vector backbone 11 kb
Selectable markers Amp^r
Gene/insert name AdV_5 - Genomic DNA
Depositor|Developer Saito, Izumu |
Other clones in our bank

External Database
AdV_5 -

            Reference sequence
              
             
            Remarks, protocol and/or map (pdf) RDB05215.pdf

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Terashima, M., Exp. Med., 21, 931-936(2003)).
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Remember that you will be working with samples containing infectious virus.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Terashima, M., Exp. Med., 21, 931-936(2003))。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

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            RDB05215 pAxEFwtit2 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pAxEFwtit2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05215).

            Reference section:

            Fukuda, H., Terashima, M., Koshikawa, M., Kanegae, Y., Saito, I., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150. [link to RRC of NBRP]
            Terashima, M., Kondo, S., Kanegae, Y., Saito, I., Exp. Med., 21, 931-936 (2003).

            Further references such as user reports and related articles (go to bottom)


            References and tips

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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB19476_B1Jfp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EF1a-C_F (Pr0168)
            Region: EF-1alpha pro,SV40 pA
            Sequence file: RDB19476_B1Jfa.seq check
            >05215_19476_B1Jf_3_EF1a-C_F_C02_08_ABI24.ab1
                1 NNNNNNNNNN NNNTTTTTNA GTTTGGNNCT TGGTTNNTTC TNNANGCCTC AGACAGTGGT
               61 TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAGGAATT AATTCATCGA TTTAAATTAT
              121 AAACTAGTCT AGAATCGATG AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
              181 ATAGCATCAC AAATTTCACA NNNNNNGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
              241 CCAAACTCAT CAATGTATCT TATCATGTCT GGATCTCGAG GGCCCAAGCT GTTTAAACGA
              301 TGCTGACTAT AATAATAAAA CGCCAACTTT GACCCGGAAC GCGGAAAACA CCTGAGAAAA
              361 ACACCTGGGC GAGTCTCCAC GTAAACGGTC AAAGTCCCCG CGGCCCTAGA CAAATATTAC
              421 GCGCTATGAG TAACACAAAA TTATTCAGAT TTCACTTCCT CTTATTCAGT TTTCCCGCGA
              481 AAATGGCCAA ATCTTACTCG GTTACGCCCA AATTTACTAC AACATCCGCC TAAAACCGCG
              541 CGAAAATTGT CACTTCCTGT GTACACCGGC GCACACNNAA AACGTCACTT TTGCCACATC
              601 CGTCGCTTAC ATGTGTTCCG CCACNCTNGC AANNTCNCAC TTNCGCCNCA CTACTACGTC
              661 NCCCNNCCCN NTTNCCNCGC CCNNNGCCNN GNCACAAANT NNNNCCCCTC NNNANNNNNN
            721 NNNN
            //
            Primer: pJB8-Forward (Pr0084)
            Region: ITR.1,Ad5 psi,
            Sequence file: RDB19476_B1Jfb.seq check
            >05215_19476_B1Jf_3_pJB8-Forward_D02_11_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNTTC NANTTAATTA ATTCGAACNT CATCAATAAT ATACCTTATT
               61 TTGGATTGAA GCCAATATGA TAATGAGGGG GTGGAGTTTG TGACGTGGCG CGGGGCGTGG
              121 GAACGGGGCG GGTGACGTAG TAGTGTGGCG GAAGTGTGAT GTTGCAAGTG TGGCGGAACA
              181 CATGTAAGCG ACGGATGTGG CAAAAGTGAC GTTTTTGGTG TGCGCCGGTG TACACAGGAA
              241 GTGACAATTT TCGCGCGGTT TTAGGCGGAT GTTGTAGTAA ATTTGGGCGT AACCGAGTAA
              301 GATTTGGCCA TTTTCGCGGG AAAACTGAAT AAGAGGAAGT GAAATCTGAA TAATTTTGTG
              361 TTACTCATAG CGCGTAATAT TTGTCTAGGG CCGCGGGGAC TTTGACCGTT TACGTGGAGA
              421 CTCGCCCAGG TGTTTTTCTC ANGTGTTTTC CGCGTTCCGG GTCAAAGTTG GCGTTTTATT
              481 ATTATAGTCA GCATCGTTTA AACAGCTTGG GCCCTCGAGA TCCAGACATG ATAAGATACA
              541 TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA
              601 TTTGTGATGC TATTGCTTTA TTTGTAACCN TTATAAGCTG CNATAAACAA GTTCNTCGAT
              661 TCTAGANNNN NTNANAANTT AAATCNNTGA NNTNATNCCN CNCNANNCCT GANNNNNNNN
            721 AAAANNNNNN NNNN
            //
            Primer: AxIT_R (PrPr347)
            Region: ITR.2
            Sequence file: RDB19476_B1Jfc.seq check
            >05215_19476_B1Jf_3_AxIT_R_H03_24_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NACTTCCTCN AATCGTCACT TCCGTTTTCC CACGTTACGT
               61 CACTTCCCAT TTTAAGAAAA CTACAATTCC CAACACATAC AAGTTACTCC GCCCTAAAAC
              121 CTACGTCACC CGCCCCGTTC CCACGCCCCG CGCCACGTCA CAAACTCCAC CCCCTCATTA
              181 TCATATTGGC TTCAATCCAA AATAAGGTAT ATTATTGATG ATGTTCGAAT TAATTAAATC
              241 GAAATCGAAC ATGCGGATCC TCTAGAGTCA ACAGCAGAAA CATACAAGCT GTCAGCTTTG
              301 CACAAGGGCC CAACACCCTG CTCATCAAGA AGCACTGTGG TTGCTGTGTT AGTAATGTGC
              361 AAAACAGGAG GCACATTTTC CCCACCTGTG TAGGTTCCAA AATATCTAGT GTTTTCATTT
              421 TTACTTGGAT CAGGAACCCA GCACTCCACT GGATAAGCAT TATCCTTATC CAAAACAGCC
              481 TTGTGGTCAG TGTTCATCTG CTGACTGTCG ACCTGCAGGC ATGCAAGCTT TAATGCGGTA
              541 GTTTATCACA GTTAAATTGC TAACGCAGTC AGGCACCGTG TATNNAAATC TAACAATGCG
              601 CTCATCGTCA TCCTCGGCNN CGTCACCCTG GNNGCTGTAG GCATNNNCNT NNNTNNGCNN
              661 GTNCNGCCNG GNCTNNNGCG GGNNANCGNC CANTNCNANN GCNNCNCCNN NNNCNNNNGG
            721 NNNGNTGNNA NCNNNNNNNG NNTNNNNGCN NTTNNNANNN NNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150. [link to RRC of NBRP]
            original Terashima, M., Exp. Med., 21, 931-936 (2003).
            user_report Araki, Y., Efficient recombinant production in mammalian cells using a novel IR/MAR gene amplification method. PLoS One, 7 (7): e41787 (2012). PMID 22844523. [link to RRC of NBRP]

            2023.06.09

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