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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAxCALNLhSTAT3(forward)

Shuttle vector to generate rAd expressing human STAT3

Catalog number RDB04699
Resource name pAxCALNLhSTAT3(forward)
Alternative name 1425C8F
Clone info. Shuttle vector to generate rAd expressing human STAT3. Cre-recombinase is required to remove stuffer sequence.
Comment Cre-recombinase is required to remove LNL stuffer sequence. Use COS-TPC method for the production of adenovirus.
Vector backbone pAxCALNLw (Cosmid, use packaging extracts for transformation)
Size of vector backbone 46074 bp
Selectable markers Amp^r
Gene/insert name Human STAT3 cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human STAT3
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature (Gene 200: 149-156, 1997) and acknowledgement to the DNA Bank are requested.
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 研究発表に際し、理研DNA Bankヘの謝辞ならびに次の論文の引用を必要とする (Gene 200: 149-156, 1997)。
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB04699 pAxCALNLhSTAT3(forward) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB04699_A4Kb.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Sequence file: RDB04699_A4Kba.seq check
          >04699_04699_A4Kb_15_pAxCA_F1_B04_04.ab1
              1 CTTCCCTGCT ACATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC AACGTGCTGG
             61 TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTGATTAA TTCGAGCGAA CGCGTATAAC
            121 TTCGTATAGC ATACATTATA CGAAGTTATC TCGAGTCGGA TTTGATCTGA TCAAGAGACA
            181 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT
            241 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC
            301 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC
            361 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC
            421 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG
            481 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC
            541 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC
            601 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT
            661 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC
            721 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG
            781 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG
            841 GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC
            901 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC
            961 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG
           1021 ACCAAGCGAC GCCCAACCCT GCCATCACGA GATTTCGATT CCACCGCCGC CCTTTCTATG
           1081 AAAGGTTGGG CTTTCGGATC CGATTTTCCG GACGCTGCTG ATGAATTCCT TCAGCGGCGG
          1141 GGATCTCAAT GGCATGTGGG GAGGTATCTT TCTCGCGCTC CAAC
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB04699_A4Kbb.seq check
          >04699_04699_A4Kb_15_pAxCALNL_F1_A04_01.ab1
              1 ACAGGCTTCT TATCATGTCT GGATCAATCC GAACGCGTAT AACTTCGTAT AGCATACATT
             61 ATACGAAGTT ATCTCGAGTC GCTCGGTACG ATTTTCGAGC ACTGTTGGCC TACTGGGTAA
            121 TTCAGCGGTT TCCGGAGCTG CGGCGGCGCA GACTGGGAGG GGGAGCCGGG GGTTCCGACG
            181 TCGCAGCCGA GGGAACAAGC CCCAACCGGA TCCTGGACAG GCACCCCGGC TTGGCGCTGT
            241 CTCTCCCCCT CGGCTCGGAG AGGCCCTTCG GCCTGAGGGA GCCTCGCCGC CCGTCCCCGG
            301 CACACGCGCA GCCCCGGCCT CTCGGCCTCT GCCGGAGAAA CAGGATGGCC CAATGGAATC
            361 AGCTACAGCA GCTTGACACA CGGTACCTGG AGCAGCTCCA TCAGCTCTAC AGTGACAGCT
            421 TCCCAATGGA GCTGCGGCAG TTTCTGGCCC CTTGGATTGA GAGTCAAGAT TGGGCATATG
            481 CGGCCAGCAA AGAATCACAT GCCACTTTGG TGTTTCATAA TCTCCTGGGA GAGATTGACC
            541 AGCAGTATAG CCGCTTCCTG CAAGAGTCGA ATGTTCTCTA TCAGCACAAT CTACGAAGAA
            601 TCAAGCAGTT TCTTCAGAGC AGGTATCTTG AGAAGCCCAA TGGAGATTGC CCGGATTGTG
            661 GCCCGGTGCC TGTGGGAAGA ATCACGCCTT CTACAGACTG CAGCCACTGC GGGCCCCAGC
            721 AAGGGGGCCA GGCCAACCAC CCCACAGCAG CCGTGGTGAC GGAGAAGCAG CATATGCTGG
            781 AGCACCACCT TCGGGATGTC CGGAACAACA GTGCAGGATC TAGAACAGAA TATGAAAGTG
            841 AGTAGAGAAT CTTCCGGAAT GACCTTTGAT TTACAACTAT AAAAGCCTTC ATCGTGTCTA
          901 ACGAGATATG GCGACATT
          //
          Primer: pAxCA_R1
          Sequence file: RDB04699_A4Kbc check
          1

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl