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CS-CDF-EG-PRE

Lentivirus vector plasmid of GFP driven by human EF-1 alpha promoter.

Catalog number RDB04380
Resource name CS-CDF-EG-PRE
Clone info. Lentivirus vector plasmid of GFP driven by human EF-1 alpha promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone Plasmid
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor|Developer Miyoshi, Hiroyuki |
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External Database

            Reference sequence
              
             
            Sequence RDB04380z.seq
            Remarks, protocol and/or map (pdf) RDB04380.pdf

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
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            Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
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            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
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            RDB04380 CS-CDF-EG-PRE DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CS-CDF-EG-PRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04380).

            Reference section:

            Kim, S., Kim, G.J., Miyoshi, H., Moon, S.H., Ahn, S.E., Lee, J.H., Lee, H.J., Cha, K.Y., Chung, H.M., Efficiency of the elongation factor-1alpha promoter in mammalian embryonic stem cells using lentiviral gene delivery systems. Stem Cells Dev. 16 (4): 537-545 (2007). PMID 17784828. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


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            Featured content

            Featured content Lentiviral plasmid list (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18955_B1Ddp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EF1a-C_F (Pr0168)
            Region: EF-1 alpha pro,EGFP 5'
            Sequence file: RDB18955_B1Dda.seq check
            >04380_18955_B1Dd_2_EF1a-Ca_F_A02_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNTTNNN NNNTTGGNNC TTGGTTNNTT CNNNNNCCTN ANANNNNGGT
               61 TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAACACGC TACCGGTCGC CACCATGGTG
              121 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
              181 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
              241 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
              301 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC
              361 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
              421 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
              481 CGCATCNAGC TGAAGGGCAT CNNCTTCAAG GAGGANGGCA ACATCCTGGG GCACAANCTG
            541 GANNANNACT ACNANNNNNN NNNN
            //
            Primer: PRE_rev (Pr0250)
            Region: PRE,EGFP 3'
            Sequence file: RDB18955_B1Ddb.seq check
            >04380_18955_B1Dd_2_PRE_rev_B02_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCNN CNTNNNTAAG AATACCAGTC AATCTTTCAC
               61 AAATTTTGTA ATCCAGAGGT TGATTATCGA TAAGCTTGAT CCCTCGAGAT CCTACTAGTA
              121 GGATCTGAGT CCGGACTTGT ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC
              181 GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG
              241 GGTGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG
              301 CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT
              361 CACCTTGATG CCGTTCTTCT GCTTGTCGGC CATGATATAG ACGTTGTGGC TGTTGTAGTT
              421 GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC
              481 GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC
              541 GTCGTCCTTG AAGAAGANGG TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA
              601 NAAGTCGTGC TGCTTCATGT GGNCGGGGNA NCGGCTGAAG CACTGCACGC CGNNNNCNNG
            661 NGGNCACNAN GGNGGNNCNN GGGCNNNGGC ANCTNNNNNN NNNN
            //
            Primer: CMV_Forward (Pr0016)
            Region: CMV pro,5' LTR,HIV-1 psi
            Sequence file: RDB18955_B1Ddc.seq check
            >04380_18955_B1Dd_2_CMV_Forward_C02_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
               61 TATAAGCAGC GCGTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG
              121 AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC
              181 TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT
              241 TTTAGTCAGT GTGGAAAATC TCTAGCAGTG GCGCCCGAAC AGGGACTTGA AAGCGAAAGG
              301 GAAACCAGAG GAGCTCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA CGGCAAGAGG
              361 CGAGGGGCGG CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT AGAAGGAGAG
              421 AGATGGGTGC GAGAGCGTCA GTATTAAGCG GGGGAGAATT AGATCGCGAT GGGAAAAAAT
              481 TCGGTTAAGG CCAGGGGGAA AGAAAAAATA TAAATTAAAA CATATAGTAT GGGCAAGCAG
              541 GGAGCTAGAA CGATTCGCAG TTAATCCTGG CCTGTTAGAA ACATCAGAAG GCTGTAGANN
              601 AATACTGGGA CAGCTACNAC CATCCCTTCA GACAGGATCA GAANAACTTA NNTNNTTATA
              661 TAATANAGTA NCNACCCTCT ATTGNGTGCA TCNAANNNNN GANNNAAAAG ANNNCNANGN
            721 ANNCTTTNNA NNNNNNNNNN
            //
            Primer: RRE-M_R (Pr0651)
            Region: RRE
            Sequence file: RDB18955_B1Ddd.seq check
            >04380_18955_B1Dd_2_RRE-M_R_D02_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNGC TGTTGCGCCT CNATAGCCCT CAGCAAATTG TTCTGCTGCT
               61 GCACTATACC AGACAATAAT TGTCTGGCCT GTACCGTCAG CGTCATTGAC GCTGCGCCCA
              121 TAGTGCTTCC TGCTGCTCCC AAGAACCCAA GGAACAAAGC TCCTATTCCC ACTGCTCTTT
              181 TTTCTCTCTG CACCACTCTT CTCTTTGCCT TGGTGGGTGC TACTCCTAAT GGTTCAATTT
              241 TTACTACTTT ATATTTATAT AATTCACTTC TCCAATTGTC CCTCATATCT CCTCCTCCAG
              301 GTCTGAAGAT CAGCGGCCGC TTGCTGTGCG GTGGTCTTAC TTTTGTTTTG CTCTTCCTCT
              361 ATCTTGTCTA AAGCTTCCTT GGTGTCTTTT ATCTCTATCC TTTGATGCAC ACAATAGAGG
              421 GTTGCTACTG TATTATATAA TGATCTAAGT TCTTCTGATC CTGTCTGAAG GGATGGTTGT
              481 AGCTGTCCCA GTATTTGTCT ACAGCCTTCT GATGTTTCTA ACAGGCCAGG ATTAACTGCG
              541 AATCGTTCTA GCTCCCTGCT TGCCCATACT ATATGTTTTA ATTTATATTT TTTCTTTCCC
              601 CCTGNCCTTA ACCGANNTTT TTCCCATCGC GNNCTNATTC TCCCCCGCTT AANACTGACG
              661 CTCTCGCACC CATCTCTCTN CNTCNANNCT CCGCTAGTCN AAATTTTTNG NGNANTCACC
            721 NNTNNNNGCC CNNNNNNNNN N
            //
            Primer: EF1a-N_R (Pr0024)
            Region: cPPT_CTS
            Sequence file: RDB18955_B1Dde.seq check
            >04380_18955_B1Dd_EF1a-N_R_G01_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN GCGNNGTGCG CTCTGCCCAC TGACGGGCAC CGGNNCCTCA
               61 CGAAGCTTGA ATTCAAAGTG AATTTCTGCT GTCCCTGTAA TAAACCCGAA AATTTTGAAT
              121 TTTTGTAATT TGTTTTTGTA ATTCTTTAGT TTGTATGTCT GTTGCTATTA TGTCTACTAT
              181 TCTTTCCCCT GCACTGTACC CCCCAATCCC CCCTTTTCTT TTAAAATTGT GGATGAATAC
              241 TGCCATTTGT AGATCCGTTC ACTAATCGAA TGGATCTGTC TCTGTCTCTC TCTCCACCTT
              301 CTTCTTCTAT TCCTTCGGGC CTGTCGGGTC CCCTCGGGGT TGGGAGGTGG GTCTGAAACG
              361 ATAATGGTGA ATATCCCTGC CTAACTCTAT TCACTATAGA AAGTACAGCA AAAACTATTC
              421 TTAAACCTAC CAAGCCTCCT ACTATCATTA TGAATAATTT TATATACCAC AGCCAATTTG
              481 TTATGTTAAA CCAATTCCAC AAACTTGCCC ATTTATCTAA TTCCAATAAT TCTTGTTCAT
              541 TCTTTTCTTG CTGGTTTTGC GATTCTTCAA TTAAGGAGTG TATTAAGCTT GTGTAANTGT
              601 TAATTTCTCT GTCCCACTCC ATCCAGGTCG TGTGANTCCA AATCTGTTCC AGANATTTAT
              661 TACTCCNACT AGCATTCCNA GGNACANNAN TGGNGCAANN GANTTTTCCN NANNNNNCCC
            721 NNANTCCCNA NGNANCNNNT NNNNN
            //
            Primer: bGH_rev3 (Pr0885)
            Region: bGH pA,3' LTR
            Sequence file: RDB18955_B1Ddf.seq check
            >04380_18955_B1Dd_2_bGH_rev3_F02_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGNN NNNNACAGAT GGCTGGCAAC TAGAAGGCAC
               61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA
              121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA
              181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT
              241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG
              301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT
              361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT
              421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT
              481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT
              541 AGATCCACAG ATCNAAGGAN ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC
              601 CCCTTTTCTT TTAAAANTGG CTAANATCTA CAGCTGCCTT GNAAGTCANT GNNCTTNNNG
            661 NACCTGANGT GNGNNTGGAA ANCNNNNTCC NNCNCCNCTT GNGCTTCNAN NNNNNNNNN
            //
            Primer: SV40pro_ori_F (Pr0731)
            Region: SV40 pro_ori,Zeo
            Sequence file: RDB18955_B1Ddg.seq check
            >04380_18955_B1Dd_2_SV40pro_ori_F_G02_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNGC CTCTGCCTCT GANCTATTCC NGAAGTAGTG AGGAGGCTTT
               61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT
              121 GATCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA
              181 AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG
              241 ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG
              301 AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG
              361 ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT
              421 ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA
              481 CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTNNNCCT GCGCGACCCG GCCGGCAACT
              541 GCGTGCACTT CGTGGCCGAN GAGCANGACT GANNCGTGCT ACGAGATTTC GATTCCNCCG
              601 CCGCCTTCTA TGAAAGGTTG GGCTTCNNAN TCGTTTTCCG GGANGCCNGC TGGATGATNN
              661 TNCNNCGCGG GNNCNCNNGC TNGNNTNCNN CGCCNNNCCC NACTTNTTNN NNNNNNNTNA
            721 NNANNNNNNN NNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kim, S., Efficiency of the elongation factor-1alpha promoter in mammalian embryonic stem cells using lentiviral gene delivery systems. Stem Cells Dev. 16 (4): 537-545 (2007). PMID 17784828. [link to RRC of NBRP]
            user_report Inagaki, S., Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. Sci. Rep. 7: 42398 (2017). PMID 28205521. [link to RRC of NBRP]
            user_report Yaguchi, M., Characterization of the properties of seven promoters in the motor cortex of rats and monkeys after lentiviral vector-mediated gene transfer. Hum. Gene Ther. Methods, 24 (6): 333-344 (2013). PMID 23964981. [link to RRC of NBRP]

            2024.03.26

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