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CSII-EF-MCS

Backbone vector plasmid of lentivirus construct with EF-1 alpha promoter.

Catalog number RDB04378
Resource name CSII-EF-MCS
Alternative name pCSII-EF-MCS
Clone info. Backbone vector plasmid of lentivirus construct with EF-1 alpha promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone Plasmid
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor|Developer Miyoshi, Hiroyuki |
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External Database

            Reference sequence
              
             
            Sequence (full) RDB04378hts01.seqcheck assembled from experimentally sequenced data
            Remarks, protocol and/or map (pdf) RDB04378.pdf
            Test sheet RDB17471_A9Hlp1-3.pdf 

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Ordering
            Order form [Credit Card Payment]  [Bank Transfer Payment]
            MTA, for use for not-for-profit academic purpose)[Word]
            Please visit Ordering instruction.[link] 
            Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB04378 CSII-EF-MCS DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]

            How to cite this biological resource

            Materials & Methods section:

            The CSII-EF-MCS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04378).

            Reference section:

            Yamaguchi, T., Morikawa, A., Miyoshi, H., Comparison of gene-trapping efficiency between retroviral and lentiviral vectors in mouse embryonic stem cells. Biochem. Biophys. Res. Commun. 425 (2): 297-303 (2012). PMID 22842569. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


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            Featured content Lentiviral plasmid list (English text)
            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            References

            Original, user report and related articles

            original Yamaguchi, T., Comparison of gene-trapping efficiency between retroviral and lentiviral vectors in mouse embryonic stem cells. Biochem. Biophys. Res. Commun. 425 (2): 297-303 (2012). PMID 22842569. [link to RRC of NBRP]
            user_report Miyamoto, M., Theilovirus 3C Protease Cleaves the C-Terminal Domain of the Innate Immune RNA Sensor, Melanoma Differentiation-Associated Gene 5, and Impairs Double-Stranded RNA-Mediated IFN Response. J. Immunol. 210 (3): 335-347 (2023). PMID 36525065. [link to RRC of NBRP]
            user_report Watanabe, K., Myeloid-associated differentiation marker is an essential host factor for human parechovirus PeV-A3 entry. Nat. Commun. 14 (1): 1817 (2023). PMID 37002207. [link to RRC of NBRP]
            user_report Sakai, E., miR-27b targets MAIP1 to mediate lipid accumulation in cultured human and mouse hepatic cells. Commun. Biol. 6 (1): 669 (2023). PMID 37355744. [link to RRC of NBRP]
            user_report Iriki, T., Senescent cells form nuclear foci that contain the 26S proteasome. Cell Rep. 42 (8): 112880 (2023). PMID 37541257. [link to RRC of NBRP]
            user_report Hayashi, Y., Nutritional and metabolic control of germ cell fate through O-GlcNAc regulation. EMBO Rep. 24 (11):
            e56845 (2023). PMID 37842859. [link to RRC of NBRP]
            user_report Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 40 (7): 1132-1142 (2022). PMID 35468954. [link to RRC of NBRP]
            user_report Kanatsu-Shinohara, M., Adeno-associated-virus-mediated gene delivery to ovaries restores fertility in congenital infertile mice. Cell Rep. Med. 3 (5): 100606 (2022) PMID 35584625. [link to RRC of NBRP]
            user_report Takizawa, M., Development of a red fluorescent protein-based cGMP indicator applicable for live-cell imaging. Commun. Biol. 5 (1): 833 (2022). PMID 36064581. [link to RRC of NBRP]
            user_report Hino, Y., Mitochondrial stress induces AREG expression and epigenomic remodeling through c-JUN and YAP-mediated enhancer activation. Nucleic Acids Res. 50 (17): 9765-9779 (2022). PMID 36095121. [link to RRC of NBRP]
            user_report Meshitsuka, S., CRISPR/Cas9 and AAV mediated insertion of β2 microglobulin-HLA-G fusion gene protects mesenchymal stromal cells from allogeneic rejection and potentiates the use for off-the-shelf cell therapy. Regen. Ther. 21: 442-452 (2022). PMID 36313397. [link to RRC of NBRP]
            user_report Morimoto, A., SLPI is a critical mediator that controls PTH-induced bone formation. Nat. Commun. 12 (1): 2136 (2021). PMID 33837198. [link to RRC of NBRP]
            user_report Kondo, N., Kindlin-3 disrupts an intersubunit association in the integrin LFA1 to trigger positive feedback activation by Rap1 and talin1. Sci. Signal. 14 (686): eabf2184 (2021). PMID 34103420. [link to RRC of NBRP]
            user_report Sima, F., Mimicking Intravasation–Extravasation with a 3D Glass Nanofluidic Model for the Chemotaxis‐Free Migration of Cancer Cells in Confined Spaces. Adv. mater. Technol. 5: 2000484 (2020). [link to RRC of NBRP]
            user_report Nakamura, T., Tissue-nonspecific alkaline phosphatase promotes the osteogenic differentiation of osteoprogenitor cells. Biochem. Biophys. Res. Commun. 524 (3): 702-709 (2020). PMID 32035618. [link to RRC of NBRP]
            user_report Imajo, M., Analysis of Retinoic Acid Receptor Signaling in Colorectal Cancer. Methods Mol. Biol. 2019: 85-93 (2019). PMID 31359390. [link to RRC of NBRP]
            user_report Nakashima, M., Trogocytosis of ligand-receptor complex and its intracellular transport in CD30 signalling. Biol. Cell 110 (5): 109-124 (2018). PMID 29431186. [link to RRC of NBRP]
            user_report Chiyonobu, N., Fatty Acid Binding Protein 4 (FABP4) Overexpression in Intratumoral Hepatic Stellate Cells within Hepatocellular Carcinoma with Metabolic Risk Factors. Am. J. Pathol. 188 (5): 1213-1224 (2018). PMID 29454748. [link to RRC of NBRP]
            user_report Kasahara, K., EGF receptor kinase suppresses ciliogenesis through activation of USP8 deubiquitinase. Nat. Commun. 9 (1): 758 (2018). PMID 29472535. [link to RRC of NBRP]
            user_report Ueda, N., Generation of TCR-Expressing Innate Lymphoid-like Helper Cells that Induce Cytotoxic T Cell-Mediated Anti-leukemic Cell Response. Stem Cell Reports 10 (6): 1935-1946 (2018). PMID 29805109. [link to RRC of NBRP]
            user_report Miura, H., Cell-to-Cell Heterogeneity in p38-Mediated Cross-Inhibition of JNK Causes Stochastic Cell Death. Cell Rep. 24 (10): 2658-2668 (2018). PMID 30184500. [link to RRC of NBRP]
            user_report Mochizuki, K., Repression of Somatic Genes by Selective Recruitment of HDAC3 by BLIMP1 Is Essential for Mouse Primordial Germ Cell Fate Determination. Cell Rep. 24 (10): 2682-2693.e6 (2018). PMID 30184502. [link to RRC of NBRP]
            user_report Yanagihashi, Y., Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis. Proc. Natl. Acad. Sci. U.S.A. 114 (33): 8800-8805 (2017). PMID 28768810. [link to RRC of NBRP]
            user_report Nakamori, D., Direct conversion of human fibroblasts into hepatocyte-like cells by ATF5, PROX1, FOXA2, FOXA3, and HNF4A transduction. Sci. Rep. 7 (1): 16675 (2017). PMID 29192290. [link to RRC of NBRP]
            user_report Otani, K., Cell-cycle-controlled radiation therapy was effective for treating a murine malignant melanoma cell line in vitro and in vivo. Sci. Rep. 6: 30689 (2016). PMID 27480052. [link to RRC of NBRP]
            user_report Shcherbakova, D.M., Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging. Nat. Commun. 7: 12405 (2016). PMID 27539380. [link to RRC of NBRP]
            user_report Matsu-Ura, T., Intercellular Coupling of the Cell Cycle and Circadian Clock in Adult Stem Cell Culture. Mol. Cell 64 (5): 900-912 (2016). PMID 27867006. [link to RRC of NBRP]
            user_report Naito, A., RFPL4A increases the G1 population and decreases sensitivity to chemotherapy in human colorectal cancer cells. J Biol Chem. 2015 Jan 20. pii: jbc.M114.614859 PMID 25605732. [link to RRC of NBRP]
            user_report Takahashi, H., Significance of Polypyrimidine Tract-Binding Protein 1 Expression in Colorectal Cancer. Mol. Cancer. Ther. 14 (7): 1705-1716 (2015). PMID 25904505. [link to RRC of NBRP]
            user_report Sato, Y., Disease modeling and lentiviral gene transfer in patient-specific induced pluripotent stem cells from late-onset Pompe disease patient. Mol. Ther. Methods Clin. Dev. 2: 15023 (2015). PMID 26199952. [link to RRC of NBRP]
            user_report Kim, Y.C., Androgen Receptor-Mediated Growth Suppression of HPr-1AR and PC3-Lenti-AR Prostate Epithelial Cells. PLoS One 10 (9): e0138286 (2015). PMID 26372468. [link to RRC of NBRP]
            user_report Morishima T., Genetic correction of HAX1 in induced pluripotent stem cells from a patient with severe congenital neutropenia improves defective granulopoiesis. Haematologica 99 (1): 19-27 (2014). PMID 23975175. [link to RRC of NBRP]
            user_report Matsumoto, S., A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures. EMBO J., 33 (7): 702-718 (2014). PMID 24562386. [link to RRC of NBRP]
            user_report Maeda, S., Interferon-alpha Acts on the S/G2/M Phases to Induce Apoptosis in the G1 Phase of an IFNAR2-Expressing Hepatocellular Carcinoma Cell Line. J. Biol. Chem. 289 (34): 23786-23795 (2014). PMID 25012666. [link to RRC of NBRP]
            user_report Kojima, K., ESCRT-0 Protein Hepatocyte Growth Factor-regulated Tyrosine Kinase Substrate (Hrs) Is Targeted to Endosomes Independently of Signal-transducing Adaptor Molecule (STAM) and the Complex Formation with STAM Promotes Its Endosomal Dissociation. J. Biol. Chem. 289 (48): 33296-33310 (2014). PMID 25296754. [link to RRC of NBRP]
            user_report Kondo, S., An HLA-modified ovarian cancer cell line induced CTL responses specific to an epitope derived from claudin-1 presented by HLA-A*24:02 molecules. Hum. Immunol., 74 (9): 1103-1110 (2013). PMID 23806269. [link to RRC of NBRP]
            user_report Inaguma, S., GLI1 Interferes with the DNA Mismatch Repair System in Pancreatic Cancer through BHLHE41-Mediated Suppression of MLH1. Cancer Res., 73 (24): 7313-7323 (2013). PMID 24165159. [link to RRC of NBRP]
            user_report Kagawa, Y., Cell Cycle-Dependent Rho GTPase Activity Dynamically Regulates Cancer Cell Motility and Invasion In Vivo. PLoS One, 8 (12): e83629 (2013). PMID 24386239. [link to RRC of NBRP]
            user_report Toyomitsu, E., CCL2 promotes P2X4 receptor trafficking to the cell surface of microglia. Purinerg. Signal, 8 (2): 301-310 (2012). PMID 22222817. [link to RRC of NBRP]
            user_report Hirota, Y., Roles of planar cell polarity signaling in maturation of neuronal precursor cells in the postnatal mouse olfactory bulb. Stem Cells, 30 (8): 1726-1733 (2012).無 PMID 22628050. [link to RRC of NBRP]
            user_report Masuda, T., IRF8 Is a Critical Transcription Factor for Transforming Microglia into a Reactive Phenotype. Cell Rep., 1 (4): 334-340 (2012). PMID 22832225. [link to RRC of NBRP]
            user_report Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP]
            user_report Amako, Y., Protein kinase D negatively regulates hepatitis C virus secretion through phosphorylation of oxysterol-binding protein and ceramide transfer protein. J. Biol. Chem., 286 (13): 11265-11274 (2011). PMID 21285358. [link to RRC of NBRP]
            user_report Ibi, M., Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein. J. Cell Sci., 124 (Pt 6): 857-864 (2011). PMID 21325031. [link to RRC of NBRP]
            user_report Hirota Y., Planar polarity of multiciliated ependymal cells involves the anterior migration of basal bodies regulated by non-muscle myosin II. Development, 137, 3037-3046 (2010). PMID 20685736. [link to RRC of NBRP]
            user_report Sasai, M., Bifurcation of Toll-like receptor 9 signaling by adaptor protein 3. Science 329 (5998): 1530-1534 (2010). PMID 20847273. [link to RRC of NBRP]
            user_report Kiriyama, M., Interaction of FLASH with arsenite resistance protein 2 is involved in cell cycle progression at S phase. Mol. Cell. Biol., 29, 4729-4741 (2009). PMID 19546234. [link to RRC of NBRP]
            user_report Shinoda Y., Efficient transduction of cytotoxic and anti-HIV-1 genes by a gene-regulatable lentiviral vector. Virus Genes., 39, 165-175 (2009). PMID 19554442. [link to RRC of NBRP]
            user_report Isogawa, M., Rearranged NF-kappa B2 gene in an adult T-cell leukemia cell line. Cancer Sci., 99 (4): 792-798 (2008). PMID 18377428. [link to RRC of NBRP]
            user_report Higuchi, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223. [link to RRC of NBRP]

            2024.03.26

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