Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMVluc+

Expression vector of firefly luciferase

Catalog number RDB03988
Resource name pCMVluc+
Alternative name pCMV-luc+
Clone info. Espression vector for firefly luc+ driven by CMV promoter. Expression was confirmed by depositor with luciferase.
Vector backbone pCMV (Plasmid)
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor|Developer Hamada, Hirofumi |
Other clones in our bank

External Database
firefly (Photinus pyralis) luciferase

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB03988 pCMVluc+ DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pCMVluc+ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03988).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


            References and tips

            Featured content

            Featured content Arranged vector (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12358_A4Amp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV-Forward ()
            Sequence file: RDB12358_A4Ama.seq check
            >03988_12358_A4Am_CMV-Foward_A10_01.ab1
                1 TTGATAAAGC TCCGCCCATG TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
               61 ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT
              121 GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGGA CTCTAGAGGA TCCGGTACTC
              181 GAGGAACTGA AAAACCAGAA AGTTAACTGG TAAGTTTAGT CTTTTTGTCT TTTATTTCAG
              241 GTCCCGGATC CGGTGGTGGT GCAAATCAAA GAACTGCTCC TCAGTGGATG TTGCCTTTAC
              301 TTCTAGGCCT GTACGGAAGT GTTACTTCTG CTCTAAAAGC TGCGGAATTG TACCCGCGGC
              361 CGCGGGAATT CGATTAGCTT GGCATTCCGG TACTGTTGGT AAAGCCACCA TGGAAGACGC
              421 CAAAAACATA AAGAAAGGCC CGGCGCCATT CTATCCGCTG GAAGATGGAA CCGCTGGAGA
              481 GCAACTGCAT AAGGCTATGA AGAGATACGC CCTGGTTCCT GGAACAATTG CTTTTACAGA
              541 TGCACATATC GAGGTGGACA TCACTTACGC TGAGTACTTC GAAATGTCCG TTCGGTTGGC
              601 AGAAGCTATG AAACGATATG GGCTGAATAC AAATCACAGA ATCGTCGTAT GCAGTGAAAA
              661 CTCTCTTCAA TTCTTTATGC CGGTGTTGGG CGCGTTATTT ATCGGAGTTG CAGTTGCGCC
              721 CGCGAACGAC ATTTATAATG AACGTGAATT GCTCAACAGT ATGGGCATTT CGCAGCCTAC
              781 CGTGGTGTTC GTTTCCAAAA AGGGGTTGCA AAAAATTTTG AACGTGCAAA AAAAGCTCCC
              841 AATCATCCAA AAAATTATTA TCATGGATTC TAAAACGGAT TACCAGGGAT TTCAGTCGAT
              901 GTACACGTTC GTCACATCTC ATCTACCTCC CGGTTTTAAT GAATACGATT TTGTGCCAGA
              961 GTCCTTCGAT AGGGACAAGA CAATTGCACT GATCATGAAC TCCTCTGGAT CTACTGGTCT
             1021 GCCTAAAGGT GTCGCTCTGC CCTCATAGAA CTGCCCTGCG TGAGATTCTC GCATGCCAGA
             1081 GATCCTATTT TTGCATCAAA TCATTTCCGG AATACTGCGA TTTTAAGTGA TGTCATTCAT
            1141 TCACGATTTT GGAATGTTTA CCTAACCACC CTCGGGA
            //
            Primer: Reverse2 ()
            Sequence file: RDB12358_A4Amb.seq check
            >03988_12358_A4Am_Reverse2_B02_05.ab1
                1 CTCATGCATT CGCCAGCTTG CATGCCTGCA GGTCGACTCT AGAGGATCCG AAAAAACCTC
               61 CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT
              121 ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA
              181 TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC
              241 TGGATCCCCG CGGCCGCGAA TTCACTAGTG ATTCTAGAAT TACACGGCGA TCTTTCCGCC
              301 CTTCTTGGCC TTTATGAGGA TCTCTCTGAT TTTTCTTGCG TCGAGTTTTC CGGTAAGACC
              361 TTTCGGTACT TCGTCCACAA ACACAACTCC TCCGCGCAAC TTTTTCGCGG TTGTTACTTG
              421 ACTGGCGACG TAATCCACGA TCTCTTTTTC CGTCATCGTC TTTCCGTGCT CCAAAACAAC
              481 AACGGCGGCG GGAAGTTCAC CGGCGTCATC GTCGGGAAGA CCTGCGACAC CTGCGTCGAA
              541 GATGTTGGGG TGTTGGAGCA AGATGGATTC CAATTCAGCG GGAGCCACCT GATAGCCTTT
              601 GTACTTAATC AGAGACTTCA GGCGGTCAAC GATGAAGAAG TGTTCGTCTT CGTCCCAGTA
              661 AGCTATGTCT CCAGAATGTA GCCATCCATC CTTGTCAATC AAGGCGTTGG TCGCTTCCGG
              721 ATTGTTTACA TAACCGGACA TAATCATAGG ACCTCTCACA CACAGTTCGC CTCTTTGATT
              781 AACGCCCAGC GTTTTCCCGG TATCCAGATC CACAACCTTC GCTTCAAAAA ATGGAACAAC
              841 TTTACCGACC GCGCCCGGTT TATCATCCCC CCTCGGGTGT AATCAGAATA GCTGATGTAG
              901 TCTCAGTGAG CCCATATCCT TGCCTGATAC CTGGCAGATG GAAACCTCTT GGCAACCGCT
              961 TCCCCGACTT CCTTTAGAGA GGGGAGCGCC ACCAGGAAGC AATTTCGTGT TAAATTAGAT
             1021 AAATCGTATT TGTCATCAGA GTTGCCTTTT TGCGAAGAGA GATAGATGGC ACAGCAGCGC
            1081 ACTTTGGAT
            //
            Primer: Luc inner_F1 ()
            Sequence file: RDB12358_A4Amc.seq check
            >03988_12358_A9EgF_20_Bp_Luc inner_F1_G07_19_ABI24.ab1
                1 GCTAGATCGA TTTTGTGCCA GAGTCCTTCG ATAGGGACAA GACAATTGCA CTGATCATGA
               61 ACTCCTCTGG ATCTACTGGT CTGCCTAAAG GTGTCGCTCT GCCTCATAGA ACTGCCTGCG
              121 TGAGATTCTC GCATGCCAGA GATCCTATTT TTGGCAATCA AATCATTCCG GATACTGCGA
              181 TTTTAAGTGT TGTTCCATTC CATCACGGTT TTGGAATGTT TACTACACTC GGATATTTGA
              241 TATGTGGATT TCGAGTCGTC TTAATGTATA GATTTGAAGA AGAGCTGTTT CTGAGGAGCC
              301 TTCAGGATTA CAAGATTCAA AGTGCGCTGC TGGTGCCAAC CCTATTCTCC TTCTTCGCCA
              361 AAAGCACTCT GATTGACAAA TACGATTTAT CTAATTTACA CGAAATTGCT TCTGGTGGCG
              421 CTCCCCTCTC TAAGGAAGTC GGGGAAGCGG TTGCCAAGAG GTTCCATCTG CCAGGTATCA
              481 GGCAAGGATA TGGGCTCACT GAGACTACAT CAGCTATTCT GATTACACCC GAGGGGGATG
              541 ATAAACCGGG CGCGGTCGGT AAAGTTGTTC CATTTTTTGA AGCGAAGGTT GTGGATCTGG
              601 ATACCGGGAA AACGCTGGGC GTTAATCAAA GAGGCGAACT GTGTGTGAGA GGTCCTATGA
              661 TTATGTCCGG TTATGTAAAC AATCCGGAAG CGACCAACGC CTTGATTGAC AAGGATGGAT
              721 GGCTACATTC TGGAGACATA GCTTACTGGG ACGAAGACGA ACACTTCTTC ATCGTTGACC
              781 GCCTGAAGTC TCTGATTAAG TACAAAGGCT ATCAGGTGGC TCCCGCTGAA TTGGAATCCA
              841 TCTTGCTCCA ACACCCCAAC ATCTTCGACG CACGTGTCGC ACGTCTTCCC GACGATGACG
              901 CCGGTGAACT TCCCGCCGCC GTTGTTGTTT TGGAGCACGG GAAGACGATG ACGGAAAAAG
              961 AGATCGTGGA TTACGTCGCC CAGTCAAGTA ACACGCGAAA AAGTTGCGCG AAGAAGTGTG
             1021 TTGTGGAACG AAGTACGGAA ACGTCCTAAC GGAAACTCGA CGCAGCAAAA TTCAGAGAGA
            1081 TTCCCTCCTA TTAAAGGTC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Nakagomi, M., A novel aromatic carboxylic acid inactivates luciferase by acylation of an enzymatically active regulatory lysine residue. PLoS One, 8 (9): e75445 (2013). PMID 24066181.

            2023.05.02

            GNP_filter3_RDBDEP_html_230501.pl