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Slac2-a

Expression vector of mouse Slp homologue lacking C2 lomains-a (Slac2a) cDNA

Catalog number RDB03422
Resource name Slac2-a
Alternative name melanophilin
Clone info. cDNA encoding the T7-tagged Slac2-a was subcloned into the NotI site of the modified pEF-BOS expression vector (J. Biol. Chem. (1994) 269, 29206-29211). Recombinant Slac2-a is localized on melanosomes through interaction with Rab27A and regulates melanosome transport in melanocytes. Expression was confirmed by depositor as follows: transfection into COS-7 cells followed by both immunoblotting and immunocytochemistry.
Vector backbone pEF-BOS (Plasmid)
Size of vector backbone 5 kbp
Selectable markers Amp^r
Gene/insert name Mouse Slac2-a/Mlph cDNA
Reference of insert sequence AB057759 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
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External Database
Mouse Slac2-a/Mlph

          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB03422.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (Biochem. Biophys. Res. Commun., 283, 513-519 (2001).; J. Biol. Chem., 277, 9212-9218 (2002).; J. Biol. Chem., 277, 12432-12436 (2002)) and an acknowledgment to DEPOSITOR are requested.
          Additional terms and conditions:
          Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Please visit Information of Request for Distribution.[link] 
          提供案内 (日本国内) [open/close]

          提供条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Biochem. Biophys. Res. Commun., 283, 513-519 (2001).; J. Biol. Chem., 277, 9212-9218 (2002).; J. Biol. Chem., 277, 12432-12436 (2002))。利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とする。
          付加的提供条件:
          pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB03422 Slac2-a Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The Slac2-a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03422).

          Reference section:

          Kuroda, T.S., Fukuda, M., Ariga, H., Mikoshiba, K., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]
          Fukuda, M., Kuroda, T.S., Mikoshiba, K., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727. [link to RRC of NBRP]
          Fukuda, M., Saegusa, C., Mikoshiba, K., Novel splicing isoforms of synaptotagmin-like proteins 2 and 3: identification of the Slp homology domain. Biochem. Biophys. Res. Commun., 283, 513-519 (2001). PMID 11327731. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          References

          Original, user report and related articles

          original Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]
          original Fukuda, M., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727. [link to RRC of NBRP]
          original Fukuda, M., Novel splicing isoforms of synaptotagmin-like proteins 2 and 3: identification of the Slp homology domain. Biochem. Biophys. Res. Commun., 283, 513-519 (2001). PMID 11327731. [link to RRC of NBRP]

          2023.08.17

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