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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Human HLA-C*06:02 cDNA

Human HLA-C*06:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03377
Resource name Human HLA-C*06:02 cDNA
Alternative name HLA-Cw*0602
Clone info. Human HLA-C*06:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*0602 cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human HLA-Cw*0602
          Reference sequence
            
           
          Sequence RDB03377z.seq
          Remarks, protocol and/or map (gif) RDB03377.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB03377 Human HLA-C*06:02 cDNA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The Human HLA-C*06:02 cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03377).

          Reference section:

          Akatsuka, Y., Goldberg, T.A., Kondo, E., Martin, E.G., Obata, Y., Morishima, Y., Takahashi, T., Hansen, J.A., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18740_B1C9p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,insert 5'
          Sequence file: RDB18740_B1C9a.seq check
          >03377_18740_B1C9_2_CMV-Forward_F03_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
             61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
            121 ACGACTCACT ATAGGGAGAC CCAAGCTGGC TAGCGTTTAA ACTTAAGCTT TTCTCCCCAG
            181 ACGCCGAGAT GCGGGTCATG GCGCCCCGAA CCCTCATCCT GCTGCTCTCG GGAGCCCTGG
            241 CCCTGACCGA GACCTGGGCC TGCTCCCACT CCATGAGGTA TTTCGACACC GCCGTGTCCC
            301 GGCCCGGCCG CGGAGAGCCC CGCTTCATCT CAGTGGGCTA CGTGGACGAC ACGCAGTTCG
            361 TGCGGTTCGA CAGCGACGCC GCGAGTCCGA GAGGGGAGCC CCGGGCGCCG TGGGTGGAGC
            421 AGGAGGGGCC GGAGTATTGG GACCGGGAGA CACAGAAGTA CAAGCGCCAG GCACAGGCTG
            481 ACCGAGTGAA CCTGCGGAAA CTGCGCGGCT ACTACAACCA GAGCGAGGAC GGGTCTCACA
            541 CCCTCCAGTG GANGTATGGC TGCGACCTNN NCCCGACGGG CGCCTCCTCC GCGGGTATGA
            601 CCAGTCCGCC TACGACGGCN AGGNNTACAT CGCCCTGAAC GANGANCTGC GCTCCNGGAN
            661 NGNCGCGGAN NNNGCGNCTN NNATNNCCNN NCGNNNGNGG NNNNNNCNNN GNNNNNNNNN
          721 N
          //
          Primer: bGH_rev3 (Pr0885)
          Region: bGHpA,insert 3'
          Sequence file: RDB18740_B1C9b.seq check
          >03377_18740_B1C9_2_bGH_rev3_G03_21_ABI24.ab1
              1 NNNNNNNNNN NNNNNGGNNG NNGNNNNNNN ACAGATGGCT GGCAACTAGA AGGCACAGTC
             61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT CTAGACTCGA GCGGCCGCGT CTCAGGCTTT
            121 ACAAGCGATG AGAGACTCAT CAGAGCCCTG GGCACTGTTG CTGGACGCAG CCTGAGAGCA
            181 GCTCCCTCCT TTTCCACCTG AGCTCTTCCT CCTACACATC ACAACAGCCA TCACAGCTCC
            241 TAGGACAGCT AGGACAGCCA GGACAGCCAG GCCAGCAACG ATGCCCACGA TGGGGATGGT
            301 GGGCTGGGAA GATGGCTCCC ATCTCAGGGT GAGGGGCTCT GGCAGCCCCT CGTGCTGCAC
            361 ATGGCACGTG TATCTCTGCT CTTCTCCAGA AGGCACCACC ACAGCTGCCC ACTTCTGGAA
            421 GGTTCCATCT CCTGCTGGCC TGGTCTCCAC AAGCTCGGTG TCCTGAGTTT GGTCCTCGCC
            481 ATCCCGCTGC CAGGTCAGTG TGATCTCCGC AGGGTAGAAG CCCAGGGCCC AGCACCTCAG
            541 GGTGGCCTCA TGGTCAGAGA CGGGATGGTG GGTCACGTGT GTCTTTGGGT GTTCCGCGCG
            601 CTGCANCGTC TCCTTCCCGT TCTCCNGGTA TCTGCGGANC CACTCCACGC ACGTGCCCTN
            661 CANGTNNNNT CTNCNCNGCT NCNNNNCNNG GNNNGNNTNC NNCTNGCGCT GGGNGNNNNN
          721 NNNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori, NeoR
          Sequence file: RDB18740_B1C9c.seq check
          >03377_18740_B1C9_2_SV40pro_ori_F_H03_24_ABI24.ab1
              1 NNNNNNNNNN NNNNGCCGCC TCTGCCTCTG ANCTATTCCN GAAGTAGTGA GGAGGCTTTT
             61 TTGGAGGCCT ANGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG
            121 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT
            181 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT
            241 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA
            301 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG
            361 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT
            421 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG
            481 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT
            541 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG
            601 GTCTTGTCGA TCANGANGAN CTGGANGAAN ANCATCNNGG GCTCGCGCCN GCCGAANNNN
            661 NNGCCNGGNT CNNNGNNCNN ATGNCCNANN NNANNNNCTC NTCNNGACCN NNNNNNANGN
          721 NNGNNNGNNN ANNNNNNNGN NNNNAANNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl