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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Human HLA-B*59:01 cDNA

Human HLA-B*59:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03375
Resource name Human HLA-B*59:01 cDNA
Alternative name HLA-B*5901
Clone info. Human HLA-B*59:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
Other clones in our bank
External Database
Human MHC class I B
          Reference sequence
            
           
          Sequence RDB03375z.seq
          Remarks, protocol and/or map (gif) RDB03375.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
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          RDB03375 Human HLA-B*59:01 cDNA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The Human HLA-B*59:01 cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03375).

          Reference section:

          Akatsuka, Y., Goldberg, T.A., Kondo, E., Martin, E.G., Obata, Y., Morishima, Y., Takahashi, T., Hansen, J.A., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13980_A6C3p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, insert 5'
          Sequence file: RDB13980_A6C3d.seq check
          >03375_13980_B0GgF_CMV-Forward_A09_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNTNNNN GCNNTGGGCG GTANGCGTGT ACGGNGGGNG GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTCACC CGGACTCAGA
            181 ATCTCCTCAG ACACCGAGAT GCGGGTCACG GCACCCCGAA CCCTCCTCCT GCTGCTCTGG
            241 GGGGCCCTGG CCCTGACCGA GACCTGGGCC GGCTCCCACT CCATGAGGTA TTTCTACACC
            301 GCCATGTCCC GGCCCGGCCG CGGGGAGCCC CGCTTCATCG CAGTGGGCTA CGTGGACGAC
            361 ACGCAGTTCG TGAGGTTCGA CAGCGACGCC GCGAGTCCGA GAGAGGAGCC GCGGGCGCCG
            421 TGGATAGAGC AGGAGGGGCC GGAGTATTGG GACCGGAACA CACAGATCTT CAAGACCAAC
            481 ACACAGACTT ACCGAGAGAA CCTGCGGATC GCGCTCCGCT ACTACAACCA GAGCGAGGCC
            541 GGGTCTCACA CTTGGCAGAC GATGTATGGC TGCGACCTGG GGCCGGACGG GCGCCTCCTC
            601 CGCGGGCATA ACCAGTTAGC CTACGACGGC AAGGNNTACA TCGCCCTGAA CGANGACCTG
            661 ANCTCCTGGA NCGCGGCGGA CACCGCGGCT CANATCACCC AGCGCAAGTG GGANGCGGCN
            721 CGNGTGGCGG ANCAGCTNAN AGCCTACCTG GNNGGCACGN GCNNGGNNTG GCTCCNCANA
            781 NACCNNGNNA ACGGGAANGA NNNGCTNCAN CGCGNNGNNC CCCCAAANNN ACNCNNNNNC
            841 NNCCNCCCCN TCTNNNANCN TNNNNNNNCC NNNANGNGCT GGNNCNNNGN NNTNNNACCN
          901 NNNNNNNNN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGH pA, insert 3'
          Sequence file: RDB13980_A6C3e.seq check
          >03375_13980_B0GgF_BGH_rev2_B09_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGNG GNNGGGCNNN CAACANNTGG CTGGCAACTA GAAGGCACAG
             61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGACTC GAGCGGCCGC ATCTCAGTCC
            121 CTCACAAGAC AGCTGTCTCA GGCTTTTCAA GCTGTGAGAG ACACATCAGA GCCCTGGGCA
            181 CTGTCGCTGG ACGCAGCCTG AGAGTAGCTC CCTCCTTTTC CACCTGAGCT CTTCCTCCTA
            241 CACATCACAG TAGCGACCAC AGCTCCGATG ACCACAACTG CTAGGACAGC CAGGCCAGCA
            301 ACAATGCCCA CGATGGGGAT GGTGGACTGG GAAGATGGCT CCCATCTCAG GGTGAGGGGC
            361 TTCGGCAGCC CCTCATGCTG TACATGGCAT GTGTATCTCT GCTCTTCTCC AGAAGGCACC
            421 ACCACAGCTG CCCACTTCTG GAAGGTTCTA TCTCCTGCTG GTCTGGTCTC CACAAGCTCA
            481 GTGTCCTGAG TTTGGTCCTC GCCATCCCGC TGCCAGGTCA GTGTGATCTC CGCAGGGTAG
            541 AAGCCCAGGG CCCAGCACCT CAGGGTGGCC TCATGGTCAG AGATGGGGTG GTGGGTCACG
            601 TGTGTCTTTG GGGGGTCCGC GCGCTGCAGC GTCTCCTTCC CGTTCTCCAG GTATCTGCGG
            661 ANCCACTCCA CGCACGTGCC CTCCAGGTAN GCTCTCAGCT GCTCCGCCAC ACGGGCCGCC
            721 TCCCACTTGC GCTGGGTGNN CTGANCCGCG GNGTCCGCCG CGGNCCANGA NCTCNNGTCC
            781 TCGTTCANGG CGATGTAATC CTTGCCGNCN NNNNNAACNN GTTATGCCCG CGGNNGANGC
          841 GCCNGNNNGG NCCCANGNCG CANCCNTACN TCNNNNGCNN NNNNNNNN
          //
          Primer: SV40pro_ori_f (Pr0731)
          Region: SV40 pro_ori, NeoR
          Sequence file: RDB13980_A6C3f.seq check
          >03375_13980_B0GgF_SV40pro_ori_F_C09_09_ABI24.ab1
              1 NNNNNNNNNN NNNGCCGCCT CTGCCTCTNN NCTATTCNNG AAGTAGTGAG GANGCTTTTT
             61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA
            121 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC
            181 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG
            241 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC
            301 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC
            361 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
            421 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA
            481 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG
            541 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG
            601 TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT
            661 CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGANGNTCTC GTCGTGACCC ATGGCGATGC
            721 CTGCTTGCCG AATATCATGG TGGAAAAATG GCCGCTTTTC TGGATTCATC GACNGTGGCC
            781 GGCNGGGNGT GGCGGACCGC TATCANGNCA TANCGTTGGC TANCCGNNAT ATNGCTGAAG
            841 ANCTNGGCNG CNAANGGNCN NANCGCNNNC TCNNGCTTTA CNNNATCGNC NCNNCNNNNN
          901 CNCANCNNNN CNNCNNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

          2023.08.18

          GNP_filter3_RDBDEP_html_230702.pl