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Human HLA-B*48:01 cDNA

Human HLA-B*48:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03372
Resource name Human HLA-B*48:01 cDNA
Alternative name HLA-B*4801
Clone info. Human HLA-B*48:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
Other clones in our bank
External Database
Human MHC class I B
          Reference sequence
            
           
          Sequence RDB03372z.seq

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
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          RDB03372 Human HLA-B*48:01 cDNA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The Human HLA-B*48:01 cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03372).

          Reference section:

          Akatsuka, Y., Goldberg, T.A., Kondo, E., Martin, E.G., Obata, Y., Morishima, Y., Takahashi, T., Hansen, J.A., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13719_A5Llp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, insert 5'
          Sequence file: RDB13719_A5Lld.seq check
          >03372_13719_B0GgF_CMV-Forward_C08_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN GNCGGTANGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG
             61 CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT ACGACTCACT
            121 ATAGGGAGAC CCAAGCTGGC TAGCGTTTAA ACTTAAGCTT CACCCGGACT CAGAATCTCC
            181 TCAGACGCCG AGATGCTGGT CATGGCGCCC CGAACCGTCC TCCTGCTGCT CTCGGCGGCC
            241 CTGGCCCTGA CCGAGACCTG GGCCGGCTCC CACTCCATGA GGTATTTCTA CACCTCCGTG
            301 TCCCGGCCCG GCCGCGGGGA GCCCCGCTTC ATCTCAGTGG GCTACGTGGA CGACACCCAG
            361 TTCGTGAGGT TCGACAGCGA CGCCGCGAGT CCGAGAGAGG AGCCGCGGGC GCCGTGGATA
            421 GAGCAGGAGG GGCCGGAGTA TTGGGACCGG GAGACACAGA TCTCCAAGAC CAACACACAG
            481 ACTTACCGAG AGAGCCTGCG GAACCTGCGC GGCTACTACA ACCAGAGCGA GGCCGGGTCT
            541 CACACCCTCC AGAGCATGTA CGGCTGCGAC GTGGGGCCGG ACGGGCGCCT CCTCCGCGGG
            601 CATAACCAGT ACGCCTACGA CGGCAAGGAT TACATCGCCC TGAACGAGGN NCTGCGCTCC
            661 TGGACCGCCG CGGACACGGC GGCTCAGATC TCCCAGCGCA NGTTGGANGC GGCCCGTGTG
            721 GCGGANCAGC TGANAGCCTA CCTGGNNGGC GANTGCGTGG ANTGGCTCCG CAGATACNTG
            781 GNNAACGGGA ANGANAAGCT GGNNCGCGCT NANCCCCCCA AAGACACACN NNNNCCANNA
            841 CCCCCATCTC NNANCNTNNN NNCNNCNNNA GGNNCNGGGN CCNNNGGNTT NNNNCCNNNC
          901 NGNNNNTNAC NCNNNNNNNN NN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGH pA, insert3'
          Sequence file: RDB13719_A5Lle.seq check
          >03372_13719_B0GgF_BGH_rev2_D08_11_ABI24.ab1
              1 NNNNNNNNNN NGNNNNNGGN NNNNNNACNG NNGNCTGGCA ACTAGAANGC ACAGTCGAGG
             61 CTGATCAGCG GGTTTAAACG GGCCCTCTAG ACTCGAGCGG CCGCATCTCA GTCCCTCACA
            121 AGACAGCTGT CTCAGGCTTT TCAAGCTGTG AGAGACACAT CAGAGCCCTG GGCACTGTCG
            181 CTGCACGCAG CCTGAGAGTA GCTCCCTCCT TTTCCACCTG AACTCTTCCT CCTACACATC
            241 ACAGCAGCGA CCACAGCTCC GATGACCACA ACTGCTAGGA CAGCCAGGCC AGCAACAATG
            301 CCCACGATGG GGACGGTGGA CTGGGAAGAC GGCTCCCATC TCAGGGTGAG GGGCTTCGGC
            361 AGCCCCTCAT GCTGTACATG GCATGTGTAT CTCTGCTCTT CTCCAGAAGG CACCACCACA
            421 GCTGTCCACT TCTGGAAGGT TCTATCTCCT GCTGGTCTGG TCTCCACAAG CTCAGTGTCC
            481 TGAGTTTGGT CCTCGCCATC CCGCTGCCAG GTCAGTGTGA TCTCCGCAGG GTAGAAACCC
            541 AGGGCCCAGC ACCTCAGGGT GGCCTCATGG TCAGAGATGG GGTGGTGGGT CACGTGTGTC
            601 TTTGGGGGGT CAGCGCGCTC CAGCTTGTCC TTCCCGTTCT CCAGGTATCT GCGGAGCCAC
            661 TCNACGCACT CGCCCTCCAG GTANGCTCTC AGCTGCTCCG CCACACGGGC CGCCTCCNAC
            721 TTGCGCTGNN ANATCTGANC CGCCGNGTCC GCGGCGGTCC AGGANCGCAN GTNNTCGTTC
            781 ANGGNNANGT AATCCTTGNC GTCNTNNNNT ACNGGNTATG CCCGCGGANG ANGNGCCCNN
            841 NNNGNCNNAC NNNNCANCCN NANNTGNNCN GGNNGGNNNG ANNNCNNNNN NNCNNNNNNG
          901 NNTNNNANNA NNNNNNNNNN
          //
          Primer: SV40pro_ori_f (Pr0731)
          Region: SV40 pro_ori, NeoR
          Sequence file: RDB13719_A5Llf.seq check
          >03372_13719_B0GgF_SV40pro_ori_F_G08_20_ABI24.ab1
              1 NNNNNNNNCC NCTGNNCTNN TCNNGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT
             61 TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG
            121 AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT
            181 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT
            241 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC
            301 CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC
            361 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA
            421 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT
            481 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA
            541 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA
            601 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC
            661 GCGCATGCCC GACGGCGANG NTCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT
            721 CATGGTGGAA NATGGCCGCT TTTCTGGATT CATCGACTGN GGCCGGCTGG GTGTGGCGGA
            781 NCGCTATNNN GNCATANCGN NGGCTANCCN GNNATATTGC TGAAGANCTN GGCGGNNAAN
            841 GGNCNNACCN CTNCCNCNNN CTTTACNNNA TCGNCNNNNC NNNTTCNCAN NNNNNCNNNN
          901 NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

          2023.08.18

          GNP_filter3_RDBDEP_html_230702.pl