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Human HLA-A*24:20 cDNA

Human HLA-A*24:20 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03365
Resource name Human HLA-A*24:20 cDNA
Alternative name HLA-A*2420
Clone info. Human HLA-A*24:20 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human major histocompatibility complex, class I, A
          Reference sequence
            
           
          Sequence (full) RDB03365zzt02.seq compiled by us

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB03365 Human HLA-A*24:20 cDNA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The Human HLA-A*24:20 cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03365).

          Reference section:

          Akatsuka, Y., Goldberg, T.A., Kondo, E., Martin, E.G., Obata, Y., Morishima, Y., Takahashi, T., Hansen, J.A., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14004_A6D4p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,insert 5'
          Sequence file: RDB14004_A6D4d.seq check
          >03365_14004_A6D4_CMV-Forward_D10_10.ab1
              1 NNNNNNNNNN NNTNGNNGCN NTGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA
             61 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC
            121 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGATTCTC CCCAGACGCC
            181 GAGGATGGCC GTCATGGCGC CCCGAACCCT CGTCCTGCTA CTCTCGGGGG CCCTGGCCCT
            241 GACCCAGACC TGGGCAGGCT CCCAATCCAT GAGGTATTTC TCCACATCCG TGTCCCGGCC
            301 CGGCCGCGGG GAGCCCCGCT TCATCGCCGT GGGCTACGTG GACGACACGC AGTTCGTGCG
            361 GTTCGACAGC GACGCCGCGA GCCAGAGGAT GGAGCCGCGG GCGCCGTGGA TAGAGCAGGA
            421 GGGGCCGGAG TATTGGGACG AGGAGACAGG GAAAGTGAAG GCCCACTCAC AGACTGACCG
            481 AGAGAACCTG CGGATCGCGC TCCGCTACTA CAACCAGAGC GAGGCCGGTT CTCACACCCT
            541 CCAGATGATG TTTGGCTGCG ACGTGGGGTC GGACGGGCGC TTCCTCCGCG GGTACCACCA
            601 GTACGCCTAC GACGGCAAGG ATTACATCGC CCTGAAAGAG GACCTGCGCT CTTGGACCGC
            661 GGCGGACATG GCGGCTCAGA TCACCAAGCG CAAGTGGGAN GCGGCCCATG TGGCGGANCA
            721 GCAGAGAGCC TACCTGGAGG GCACGTGCGT GGACGGGCTC CGCAGATACC TGGAGAACGG
            781 GAANGAGNNG CTGCAGCGCA CGGACCCCCC CNAGACACAT ATGACCCACC ACCNCNTCTC
            841 TGACCNTGAN GCCACTCTGA GATGCTGGGC CCTGGGCTTC NANCCTGCGG NNNNNACACN
            901 NNNCTGGCAG CGGGNTGGGG GANGNNCAGA NCCNGGANAC NGNNCNNGTG GNNANCANGC
            961 CNNNNNNGNN NNGNNNNCNT TNCNGNNNNN GNNNNCNNNN GNNNGNNNNC NNTNNNNNNN
          1021 NNN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGH pA,insert 3'
          Sequence file: RDB14004_A6D4e.seq check
          >03365_14004_A6D4_BGH_rev2_E10_13.ab1
              1 NNNNNNNNNN NNNGGGGGNN GGNNNNNNAC AGATGGCTGG CAACTAGAAG GCACAGTCGA
             61 GGCTGATCAG CGGGTTTAAA CGGGCCCTCT AGACTCGAGC GGCCGCACAA GGCAGCTGTC
            121 TCACACTTTA CAAGCTGTGA GAGACACATC AGAGCCCTGG GCACTGTCAC TGCTTGCAGC
            181 CTGAGAGTAG CTCCCTCCTT TTCTATCTGA GCTGTTCCTC CTCCACATCA CAGCAGCGAC
            241 CACAGCTCCA GTGATCACAG CTCCAAGGAG AACCAGGCCA GCAATGATGC CCACGATGGG
            301 GACGGTGGGC TGGGAAGATG GCTCCCATCT CAGGGTGAGG GGCTTGGGCA GACCCTCATG
            361 CTGCACATGG CAGGTGTATC TCTGCTCCTC TCCAGAAGGT ACCACCACAG CTGCCCACTT
            421 CTGGAAGGTT CCATCCCCTG CAGGCCTGGT CTCCACAAGC TCCGTGTCCT GGGTCTGGTC
            481 CTCCCCATCC CGCTGCCAGG TCAGTGTGAT CTCCGCAGGG TAGAAGCCCA GGGCCCAGCA
            541 TCTCAGAGTG GCCTCATGGT CAGAGATGGG GTGGTGGGTC ATATGTGTCT TGGGGGGGTC
            601 CGTGCGCTGC AGCGTCTCCT TCCCGTTCTC CAGGTATCTG CGGAGCCCGT CCACGCACGT
            661 GCCCTCCAGG TAGGCTCTCT GCTGCTCCGC CACATGGGCC GCCTCCCACT TGCGCTTGGT
            721 GATCTGAGCC GCCATGTCCG CCGCGGTCCN AGAGCGCANG TCCTCTTTCA NGGCGATGTA
            781 ATCCTTGCCG TCGTANGCGT ACTGGTGGTA CNCGCGGANG AANCGCCCGT CCGACCNCAC
            841 GTCGCANCCA AACATCATCT GGANNGTGTG ANAANCGGCC NCGCNCNNGT TGTAGTANCG
            901 GANCNCGATN CNCNNNNTNN NNNGGNCANN CTGNNANNNG GCNNNNNNNT TNCCNGNNNN
          961 NNNNNN
          //
          Primer: SV40pro_F_V2 (Pr0154)
          Region: NeoR
          Sequence file: RDB14004_A6D4f.seq check
          >03365_14004_A6D4_SV40pro_F_V2_F10_16.ab1
              1 NNNNNNNNNN NGNNNNNNNG CTTTTGCAAA AGCTCCCGGG NNCTTGTATA TCCNTTTTCG
             61 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
            121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
            181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
            241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT
            301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
            361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
            421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
            481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
            541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG
            601 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG
            661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA NTCATCGACT
            721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC ANGACATAGC GTTGGCTACC CGTGATATTG
            781 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC
            841 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCNTGANGA NTTCTTCTGA NCGGGANTCT
            901 NGGGNTCGAA ATGANCGANN NANCGACGCC CAACNNGNNN TCNCGANANT TCGANTNNNN
          961 CGNNGNNNNC TATNNANNNN NNNGNNNNNN GNNNNNNNTT NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl