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crb2+

Plasmid clone of fission yeast Crb2, SPBC342.05

Catalog number RDB02950
Resource name crb2+
Alternative name rhp9+
Clone info. Plasmid clone of fission yeast (S. pombe) Crb2, a checkpoint protein.
Vector backbone pBluescript KS(+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name S. pombe crb2 Genomic DNA
Reference of insert sequence D86478 (DDBJ accession number)
Depositor|Developer Yanagida, Mitsuhiro |
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External Database
S. pombe crb2
          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB02950.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Genes & Dev., 11, 3387-3400 (1997)).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Genes & Dev., 11, 3387-3400 (1997))。
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          手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

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          RDB02950 crb2+ DNA solution Please contact us.

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The crb2+ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02950).

          Reference section:

          Saka, Y., Esashi, F., Matsusaka, T., Mochida, S., Yanagida, M., Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2, a protein with BRCT motif, with Cut5 and Chk1. Genes & Dev., 11, 3387-3400 (1997). PMID 9407031. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13008_A4Ka.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB13008_A4Kaa.seq check
          >02950_13008_A4Ka_2_M13_-40_B10_04.ab1
              1 AAGCCCGCGA TTGTATACGA CTCACTATAG GGCGAATTGG AGCTCCACCG CGGTGGCGGC
             61 CGCTCTAGAA CTAGTGGATC CCCCGGGCTG CAGTGATCGG ACGGGAACTG GTGTTTTCGC
            121 CTAGGTATGG CCGTAGACAG CTGTTAAGTT TTAATAAAGT ATATAAACGA TAGTTATCAC
            181 TCATTAGGAA TTCGAATGTA TGTTGGCTCC ACTACGAATA AGATATGAAT GTTAGCAATA
            241 TAACCTGAAA CTATATTTGA CATTCTTTTT TTAGAAATAT CATTAAACAA GCAAAATCTA
            301 TTTTCTATGA TTGTTTCAAA AAGTCAAGAT GAAAAGTTCA AAACTCAAAT TTAAAATTAC
            361 TTCTTCAACA TTACTGATTC TAACAACATA AATCTCCTAT CGAACGTATT AATAAAAGTG
            421 GAAACATGCT GTAGTTTGTA CATCAGGTCA AACAATTATT AATAAAATTA GAAAATAAGT
            481 TTTTCTACCT TGAGCCCTTC ATTTCCATAT TAATATATAT ATATATATGA TTAGCCAAAT
            541 AGCTGGTGTT GTAACTAAAC GTTCTTAACT GCTTTCATAT AAAGTAACGG TGCAACGTGT
            601 TACTTATGCA GAGCTTACAA CAGATAAATC GAAATTTGGG TTAAGCCCTG GTTAAATTTG
            661 TAGTTCTGAC AATTGTGAGG TATTTTAGAT GTTTTCAATA TTTTGTTTGA AAGTTTAACA
            721 ATATTATGGA GGTGAATGAT ACCTCTTTGC ATAAAGGATT TGGCCTTGAC ATAAATTCTC
            781 AACGTGTATT TGGAGCACAA GCAGCAATTT CTCGTAACAA TTATTCAAAA GTGAATGCTT
            841 CAATTAATCC TTCCCCTCCT AGGAGTAATG ATAATTCTAA TAAGGAGTTT AGTTATTCTA
            901 AGGATGTGAA TAACAATGGT GCTGTTGAGG AGCTAAGTCT TACGCAGTTG TTTGAAGTAC
            961 CTAGTCAAGC TGCTTTTGCA AAACAGAGTT CCCAGGACAT TTCGGATGAT GAACTTATTC
           1021 AACATGATTC TCGTAAGTGA TATCGTCGCC CTTATTCACC CAGCAGACAC ATACAGTGTT
           1081 TAAAACGCTT ATACGATCGG TCATCAGTAT TATCGACCAT GAAAGCTGTG GACTTCTTCA
          1141 GATGTATCTA AATCCTTTCC AAATTCCCTT TTCCCCCAAT
          //
          Primer: Reverse2
          Sequence file: RDB13008_A4Kab.seq check
          >02950_13008_A4Ka_2_Reverse2_E10_13.ab1
              1 AAGGTAAAAT GATTACGCCA AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT
             61 ACCGGGCCCC CCCTCGCTAG CATACCTTGC AAACGATTCA ATATCACAAG TTTGAATCGA
            121 GACAAGTACA CCGTATTCAA ATACATTTCC TATAAGGAAG ATTAGAGGTC TGTTCTTAGA
            181 TAACTATACA AATAACATAC TGGCAAAACT AGCTTTCTCT TTATCGCTAG TAGGGACGAA
            241 GAGGTTCTGC TTAGCAAGTT CGACTTTCAG TTTCAGAAGT TCCTTTTTAC AAGCATTCCA
            301 ATCCTTTCTG TCATAAAGAT CTGCTAAATG GTTCAAGTCT AATGTACTCA TATTGATGTG
            361 TATTGAGAGT GTAATAAGTT GGAACTTTTA AGAGAGCTGT AGAAACAACA CCTCTTGCCA
            421 TGCATCTCAG AATAGTCATC AATAACGAAC ACACTATAAA GGTTTACCAG CAAAAATTAA
            481 AGCTCCTTAC GGATTTTGAC GTTGTTTTAT TAGATTCATT ATATCACTTA AGCAATTGTA
            541 ACATAGTATT CACTCACAAT AATTGCTGTA GTTGCGTTAA CTCTCTTTAT CCTTTAAACT
            601 AAAGAATATG CCGGATCTTT GGTCAGAAAG TATATTAGAA GGCAGAAAAT TGAGTATTTA
            661 TCATCTTAAA AGTACTTTAG AGAAATGTCC ATTTCTATTT GGTCAAAGTA AGAAGTCTAA
            721 GGATTTTCAA TTTGGGAGTC ATCTTTTTTT GGTAGAGGAA CAAAATGTAT TTATTTTTGG
            781 CATGGAATGC ATAGTTTACG AAAAAAACAA AGAGTTTATA GTATTTGTAT CTAAAGCAGA
            841 TTCTACCGGA TTCGGCAGCA AAGGGGTATC TTGTAACTCT CTTGCTTTTT GCTGCTTAGT
            901 GACACTTATT GACGGTCTTA GAAAACAAGG AGCCGAAAAT GTAACTTTGA CTCTGTTTGC
            961 GATTGCTCAA GGTCAATATT TATTTCCTGA GTCGGTGGAC AATGGACAAA AGCATGTGCT
           1021 GAATGATTCT GGGTTTGTTA CGCTGGGTGG GGTAAATTGT TGCAAAAACC TCAGAGTATT
           1081 TATACTGACA GTGAGCACCA ACGATTCCCG GAAAGCCAAA GATCCCACAT AACTCCTAGC
          1141 CTTAATTTTA ATTTGTTTCC CTGTGACCTG A
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Saka, Y., Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2, a protein with BRCT motif, with Cut5 and Chk1. Genes & Dev., 11, 3387-3400 (1997). PMID 9407031. [link to RRC of NBRP]

          2023.08.18

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