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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Human HLA-B*40:01:02 cDNA

Human HLA-B*40:01:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02877
Resource name Human HLA-B*40:01:02 cDNA
Alternative name HLA-B*40012
Clone info. Human HLA-B*40:01:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
Other clones in our bank
External Database
Human MHC class I B
          Reference sequence
            
           
          Sequence RDB02877z.seq
          Remarks, protocol and/or map (gif) RDB02877.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
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          RDB02877 Human HLA-B*40:01:02 cDNA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The Human HLA-B*40:01:02 cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02877).

          Reference section:

          Akatsuka, Y., Goldberg, T.A., Kondo, E., Martin, E.G., Obata, Y., Morishima, Y., Takahashi, T., Hansen, J.A., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13916_A6Aqp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, insert 5'
          Sequence file: RDB13916_A6Aqc.seq check
          >02877_13916_B0GgF_CMV-Forward_C07_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNTGGGCG GTANGCGTGT ACGGTGGGAN GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTCACC CGGACTCAGA
            181 ATCTCCTCAG ACGCCGAGAT GCGGGTCACG GCACCCCGAA CCGTCCTCCT GCTGCTCTCG
            241 GCGGCCCTGG CCCTGACCGA GACCTGGGCC GGCTCCCACT CCATGAGGTA TTTCCACACC
            301 GCCATGTCCC GGCCCGGCCG CGGGGAGCCC CGCTTCATCA CCGTGGGCTA CGTGGACGAC
            361 ACGCTGTTCG TGAGGTTCGA CAGCGACGCC ACGAGTCCGA GGAAGGAGCC GCGGGCGCCA
            421 TGGATAGAGC AGGAGGGGCC GGAGTATTGG GACCGGGAGA CACAGATCTC CAAGACCAAC
            481 ACACAGACTT ACCGAGNNAG CCTGCGGAAC CTGCGCGGCT ACTACAACCA GAGCGAGGCC
            541 GGGTCTCACA CCCTCCAGAG GATGTACGGC TGCGACGTGG GGCCGGACGG GCGCCTCCTC
            601 CGCGGGCATA ACCAGTACGC CTACGACGGC NAGGNNTACA TCGCCCTGAA CGANGNNCTG
            661 CGCTCCTGGA CCGCCGCGGA CACGGCGGCT CANATCTCCC AGCGCANGTT GGANGCGGCC
            721 CGTGTGGCGG ANCAGCTGAN NNCCTACCTG GNNGGCGANT GCGNGGNNNG GCTCCNCAGA
            781 TACCTGNANA ACGGGAANGN NAAGCTNGNN CGCGCNNACC CCCCCNAAGN NNCNCNNNNC
            841 CNNCCNCCCC NNTCNNNNAN NNNNNNNCCN CCNNNNNNNN CNGGNNCNNN GNNTNNNNNN
          901 NNNN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGH pA, insert 3'
          Sequence file: RDB13916_A6Aqd.seq check
          >02877_13916_B0GgF_BGH_rev2_D07_10_ABI24.ab1
              1 NNNNNNNNNN NNNGGNGGNN GGGCNNNCAA CAGNTGGCTG GCAACTAGAA GGCACAGTCG
             61 AGGCTGATCA GCGGGTTTAA ACGGGCCCTC TAGACTCGAG CGGCCGCATC TCAGTCCCTC
            121 ACAAGACAGC TGTCTCAGGC TTTTCAAGCT GTGAGAGACA CATCAGAGCC CTGGGCACTG
            181 TCGCTGCACG CAGCCTGAGA GTAGCTCCCT CCTTTTCCAC CTGAACTCTT CCTCCTACAC
            241 ATCACAGCAG CGACCACAGC TCCGATGACC ACAACTGCTA GGACAGCCAG GCCAGCAACA
            301 ATGCCCACGA TGGGGACGGT GGACTGGGAA GACGGCTCCC ATCTCAGGGT GAGGGGCTTC
            361 GGCAGCCCCT CATGCTGTAC ATGGCATGTG TATCTCTGCT CTTCTCCAGA AGGCACCACC
            421 ACAGCTGCCC ACTTCTGGAA GGTTCTATCT CCTGCTGGTC TGGTCTCCAC AAGCTCAGTG
            481 TCCTGAGTTT GGTCCTCGCC ATCCCGCTGC CAGGTCAGTG TGATCTCCGC AGGGTAGAAA
            541 CCCAGGGCCC AGCACCTCAN GGTGGCCTCA TGGTCAGAGA TGGGGTGGTG GGTCACGTGT
            601 GTCTTTGGGG GGTCAGCGCG CTCCAGCTTG TCCTTCCCGT TCTCCAGGTA TCTGCGGANC
            661 CACTCCACGC ACTCGCCCTC CAGGTANGCT CTCANCTGCT CCGCCACACG GGCCGCCTCC
            721 NACTTGCGCT GGGNNANCTG ANCCGCCGTG TNCGCGGCGG TCCANGANCG CNNNCCTCGT
            781 TCNNGGCGAT GTAATCCTTG CCGTCNNNNN GTACNGGTNN TGCCCGCGGA NGANGNGCCN
            841 NNNNNGNCCC NCNNCNCANN NNNACNTCCN NNNNNNGGNN NNANANCCNG NCNTCNNNNN
          901 NGNNNNNNNN NN
          //
          Primer: SV40pro_F (Pr0110)
          Region: SV40 peo_ori, NeoR
          Sequence file: RDB13916_A6Aqe.seq check
          >02877_13916_B0GgF_SV40pro_ori_F_E07_13_ABI24.ab1
              1 NNNNNNNNNN NNNCCGCCNC TGCCTCTGAN CTATTCNNGA AGTAGTGAGG AGGCTTTTTT
             61 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT
            121 CAAGAGACAG GATGAGGNTC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT
            181 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC
            241 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC
            301 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC
            361 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG
            421 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG
            481 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC
            541 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT
            601 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC
            661 GCCAGGCTCN AGGCGCGCAT GCCCGACGGC GANGNTCTCG TCGTGACCCA TGGCGATGCC
            721 TGCTTGCCGA ATATCATGGN GGAAANTGGN CGCTTTNCTG GANTCATCNN CNGNGGCCGG
            781 CNGGGNGTGG CNGNNCGCTN TCNNNANNTA NCGTTNGCTA NNNNNNANNT TGCNGNNNNN
          841 CTNGNNGGNN NANNGGGNNN NNNGNNTNCN NCNNNNNTNN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

          2023.08.18

          GNP_filter3_RDBDEP_html_230702.pl