RDB02876 - Human HLA-B*35:01:01 cDNA

Resource data sheet
Prev.
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

Human HLA-B*35:01:01 cDNA

Human HLA-B*35:01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number	RDB02876
Resource name	Human HLA-B*35:01:01 cDNA
Alternative name	HLA-B*35011
Clone info.	Human HLA-B*35:01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone	pcDNA3.1(+) (Plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name	Human MHC class I B cDNA
Depositor\|Developer	Akatsuka, Yoshiki \|

Other clones in our bank

human MHC class I B

External Database
Human MHC class I B

Reference sequence

Sequence (full)	RDB02876zzt02.seq compiled by us
Remarks, protocol and/or map (gif)	RDB02876.gif

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).

Ordering
Order form [Credit Card Payment] [Bank Transfer Payment]
MTA, for use for not-for-profit academic purpose [Word]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

提供案内 (日本国内) [open/close]

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB02876	Human HLA-B*35:01:01 cDNA	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Human HLA-B*35:01:01 cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02876).

Reference section:

Akatsuka, Y., Goldberg, T.A., Kondo, E., Martin, E.G., Obata, Y., Morishima, Y., Takahashi, T., Hansen, J.A., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

Tips

Featured content	HLA Clones (en) (English text)
Featured content	HLA Clones (ja) (Japanese text)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB19821_B2L6p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5' Sequence file: RDB19821_B2L6a.seq
>02876_19821_B2L6_1_CMV_Forward_A01_01_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGNNNNNNN NNNNNNNNNN 61 NNNNGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT 121 ACGACTCACT ATAGGGAGAC CCAAGCTGGC TAGCGTTTAA ACTTAAGCTT CACCCGGACT 181 CAGAATCTCC TCAGACGCCG AGATGCGGGT CACGGCGCCC CGAACCGTCC TCCTGCTGCT 241 CTGGGGGGCA GTGGCCCTGA CCGAGACCTG GGCCGGCTCC CACTCCATGA GGTATTTCTA 301 CACCGCCATG TCCCGGCCCG GCCGCGGGGA GCCCCGCTTC ATCGCAGTGG GCTACGTGGA 361 CGACACCCAG TTCGTGAGGT TCGACAGCGA CGCCGCGAGT CCGAGGACGG AGCCCCGGGC 421 GCCATGGATA GAGCAGGAGG GGCCGGAGTA TTGGGACCGG AACACACAGA TCTTCAAGAC 481 CAACACACAG ACTTACCGAG AGAGCCTGCG GAACCTGCGC GGCTACTACA ACCAGAGCGA 541 GGCCGGGTCT CACATCATCC AGAGGATGTA TGGCTGCGAC CTGGGGCCCG ACGGGCGCCT 601 CCTCCGCGGG CATGACCAGT CCGCCTACGA CGGCAAGGAT TACATCGCCC TGAACGAGGA 661 CCTGAGCTCC TGGACCGCGG CGGACACCGC GGCTCAGATC ACCCAGCGCA AGTGGGANGC 721 GGCCCGTGTG GCGGANCAGC TGANAGCCTA CCTGGAGGGN CTGTGCGTGG AGTGGCTCCG 781 CAGATACCTG GAGAACGGGA AGGAGANGCT GCAGCGCGCG GANCCCCCCA AAGACACACG 841 TGACNCACCA CCCCNTCTCT NNNCATGANG NCACCNNNNN GNGCTNGGCC CNNGGCTTCT 901 ANCCNGCNGA NNTCACACNG NNCNNNNNNN NGGNTGGNNN GGNNNNAANN CNNNANNNTN 961 NNCNNGNNNN NNNNNN //
Primer: BGH_rev3 (Pr0885) Region: bGH pA,insert 3' Sequence file: RDB19821_B2L6b.seq
>02876_19821_B2L6_1_bGH_rev3_B01_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NGNNGNNGGN NNNNNACAGA TGGCTGGCAA CTAGAAGGCA 61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTCTAGA CTCGAGCGGC CGCATCTCAG 121 TCCCTCACAA GACAGCTGTC TCAGGCTTTT CAAGCTGTGA GAGACACATC AGAGCCCTGG 181 GCACTGTCGC TGGACGCAGC CTGAGAGTAG CTCCCTCCTT TTCCACCTGA GCTCTTCCTC 241 CTACACATCA CAGTAGCGAC CACAGCTCCG ATGACCACAA CTGCTAGGAC AGCCAGGCCA 301 GCAACAATGC CCACGATGGG GATGGTGGAC TGGGAAGATG GCTCCCATCT CAGGGTGAGG 361 GGCTTCGGCA GCCCCTCATG CTGTACATGG CATGTGTATC TCTGCTCTTC TCCAGAAGGC 421 ACCACCACAG CTGCCCACTT CTGGAAGGTT CTATCTCCTG CTGGTCTGGT CTCCACAAGC 481 TCAGTGTCCT GAGTTTGGTC CTCGCCATCC CGCTGCCAGG TCAGTGTGAT CTCCGCAGGG 541 TAGAAGCCCA GGGCCCAGCA CCTCAGGGTG GCCTCATGGT CAGAGACGGG GTGGTGGGTC 601 ACGTGTGTCT TTGGGGGGTC CGCGCGCTGC AGCGTCTCCT TCCCGTTCTC CAGGTATCTG 661 CGGAGCCACT CCACGCACAG GCCCTCCAGG TAGGCTCTCA GCTGCTCCGC CACACGGGCC 721 GCCTCCCACT TGCGCTGGGT GATCTGAGCC GCGGTGTCCG CCGCGGTCCA NGANCTCANG 781 TCCTCGTTCA NGGCGATGTA ATCCTTGCCG TCNTANGCGG ACTGGTCATG CCCGCGGANG 841 ANGCGCCCGT CNGGCCCCAG GTCGCAGCCA TACATCCTCN NGNNNGATGT GAGANCCGGC 901 CTCGCTCTGG TTGTANTANC CGCGNNNNNT TNCGNNNNNN TCTCTCNGNT AANTCTGTGT 961 GTTNGGNCNT GAAGATCNGN GTGNTNNCNN NCCNNNNANN CNNGNNCCNN NCNNNNNNNN 1021 NN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB19821_B2L6c.seq
>02876_19821_B2L6_1_SV40pro_ori_F_C01_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNC GCCTCTGCCT CTGANCTATT CNNGAAGTAG TGAGGAGGCT 61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT 121 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG 181 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG 241 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA 301 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC 361 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG 421 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG 481 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA 541 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG 601 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC 661 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG 721 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG 781 GCCGGCTGGG TGTGGCGGAN CGCTATCNNG ACATAGCGTT GGCTACNCGN GATATTGCTG 841 AAGANCTNGG CGGCGAATGG GCTGACCGCT NCCTCNNGCT TTACGGNATC GCCGCTCCCN 901 ATNCNCAGCG CATCGCCTTC NATCGNNNNC TTGANNANTN CNNCNNNNCG GGNNNNNNGG 961 NNNTNNNNAN NNNNNNNNCN NNNCNNNNNC NNANNCNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

2024.03.26

HTML_filter_RDB_240326.pl