RDB02874 - Human HLA-B*07:02:01 cDNA

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Human HLA-B*07:02:01 cDNA

Human HLA-B*07:02:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number	RDB02874
Resource name	Human HLA-B*07:02:01 cDNA
Alternative name	HLA-B*07021
Clone info.	Human HLA-B*07:02:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone	pcDNA3.1(+) (Plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name	Human MHC class I B cDNA
Depositor\|Developer	Akatsuka, Yoshiki \|

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human MHC class I B

External Database
Human MHC class I B

Reference sequence

Sequence	RDB02874z.seq
Remarks, protocol and/or map (gif)	RDB02874.gif

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB02874	Human HLA-B*07:02:01 cDNA	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Human HLA-B*07:02:01 cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02874).

Reference section:

Akatsuka, Y., Goldberg, T.A., Kondo, E., Martin, E.G., Obata, Y., Morishima, Y., Takahashi, T., Hansen, J.A., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

Tips

Featured content	HLA Clones (en) (English text)
Featured content	HLA Clones (ja) (Japanese text)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13690_A5Jgp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, insert 5' Sequence file: RDB13690_A5Jga.seq
>02874_13690_A5Jg_CMV-Forward_G04_19.ab1 1 NNNNNNNNNN NNNNNTNNNG CNNNGGGCGG TANGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC 121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTCACCC GGACTCAGAA 181 TCTCCTCAGA CGCCGAGATG CTGGTCATGG CGCCCCGAAC CGTCCTCCTG CTGCTCTCGG 241 CGGCCCTGGC CCTGACCGAG ACCTGGGCCG GCTCCCACTC CATGAGGTAT TTCTACACCT 301 CCGTGTCCCG GCCCGGCCGC GGGGAGCCCC GCTTCATCTC AGTGGGCTAC GTGGACGACA 361 CCCAGTTCGT GAGGTTCGAC AGCGACGCCG CGAGTCCGAG AGAGGAGCCG CGGGCGCCGT 421 GGATAGAGCA GGAGGGGCCG GAGTATTGGG ACCGGAACAC ACAGATCTAC AAGGCCCAGG 481 CACAGACTGA CCGAGAGAGC CTGCGGAACC TGCGCGGCTA CTACAACCAG AGCGAGGCCG 541 GGTCTCACAC CCTCCAGAGC ATGTACGGCT GCGACGTGGG GCCGGACGGG CGCCTCCTCC 601 GCGGGCATGA CCAGTACGCC TACGACGGCA AGGATTACAT CGCCCTGAAC GAGGACCTGC 661 GCTCCTGGAC CGCCGCGGAC ACGGCGGCTC AGATCACCCA GCGCAAGTGG GANGCGGCCC 721 GTGAGGCGGA NCAGCGGANA GCCTACCTGG AGGGCGAGTG CGTGGAGTGG CTCCGCAGAT 781 ACCTGGAGAA CGGGAAGGAN AAGCTGGANC GCGCTGANCC CCCNAAGANA CACGTGANCC 841 ACCACCCCNT CTCTGANNNT GANGNCACCC NNNNGTGCTG GGCCCNNGGT TTCTACCNTG 901 CGGANATNNC NNNNNNCNNN NNNCGGNNTG NNGANGANNN AANTNNNNAN NNTNNNNNTN 961 NNNNANANNA GANNANCNNN NANNNNNNNN TNNNNNNNNN N //
Primer: BGH_rev2 (Pr0606) Region: bGH pA, insert 3' Sequence file: RDB13690_A5Jgd.seq
>02874_13690_B0GgF_BGH_rev2_C08_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNGGGG GNNGGNNNNN NACAGATGGC TGGCAACTAG AANGCACAGT 61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGACTCG AGCGGCCGCA TCTCAGTCCC 121 TCACAAGACA GCTGTCTCAG GCTTTTCAAG CTGTGAGAGA CACATCAGAG CCCTGGGCAC 181 TGTCGCTGCA CGCAGCCTGA GAGTAGCTCC CTCCTTTTCC ACCTGAACTC TTCCTCCTAC 241 ACATCACAGC AGCGACCACA GCTCCGATGA CCACAACTGC TAGGACAGCC AGGCCAGCAA 301 CAATGCCCAC GATGGGGACG GTGGACTGGG AAGACGGCTC CCATCTCAGG GTGAGGGGCT 361 TCGGCAGCCC CTCATGCTGT ACATGGCATG TGTATCTCTG CTCTTCTCCA GAAGGCACCA 421 CCACAGCTGC CCACTTCTGG AAGGTTCTAT CTCCTGCTGG TCTGGTCTCC ACAAGCTCAG 481 TGTCCTGAGT TTGGTCCTCG CCATCCCGCT GCCAGGTCAG TGTGATCTCC GCAGGGTAGA 541 AACCCAGGGC CCAGCACCTC AGGGTGGCCT CATGGTCAGA GATGGGGTGG TGGGTCACGT 601 GTGTCTTTGG GGGGTCAGCG CGCTCCAGCT TGTCCTTCCC GTTCTCCAGG TATCTGCGGA 661 NCCACTCCAC GCACTCGCCC TCCAGGTANG CTCTCCGCTG CTCCGCCTCA CGGGCCGCCT 721 CCCACTTGCG CTGGGTGNTC TGANCCGCCG TGTCCGCGGC GGTCCAGGAN CGCANGTCCT 781 CGTTCANGGC GATGTNATCC TTGCCGTCGN NNNGTACNNG NCATGCCCGC GGNNNNNGCG 841 CCCGNCCNGC CCCACNTCGC ANCNNNACAT NCTNNNGNNG GNGNNANANC NNGCCNNNNN 901 NNNNN //
Primer: SV40pro_ori_f (Pr0731) Region: SV40 pro_ori, NeoR Sequence file: RDB13690_A5Jge.seq
>02874_13690_B0GgF_SV40pro_ori_F_D08_11_ABI24.ab1 1 NNNNNNNNNN NNGNCGCCNC TGCCTCTGNN CTATTCNNGA AGTAGTGAGG ANGCTTTTTT 61 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 121 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 181 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 241 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 301 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 361 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 421 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 481 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 541 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 601 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 661 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GANGNTCTCG TCGTGACCCA TGGCGATGCC 721 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CNGNGGCCGG 781 CNGGGTGTGG CGGNNCGCTA TCANGNNATA NCGTTGGCNA NCCNNNANAT NGCTGAAGAN 841 CTNNGCGGCN AANGGNNNNA NNGCNNCNNC NNNNNTTACN GNATCNNNNN NNCNNNNTNN 901 NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

2024.03.26

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