icon below as well as clone information before placing your order.
Mammalian vector expressing wild type p53 gene
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Other clones in our bank
External Database Human tumor protein p53 Reference sequence |
| Sequence | RDB02429y.seq |
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| Sequence | RDB02429z.seq |
| Remarks, protocol and/or map (gif) | RDB02429.gif
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested. |
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| Ordering | Order form [Credit Card Payment  ] [Bank Transfer Payment]MTA, for use for not-for-profit academic purpose [Word ]Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
[open/close]| 提供条件 | 使用にあたっては常に密な連絡をとり、発表等に関しても事前に合意を要する。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。 |
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| 提供依頼 | 提供依頼書 [Word ]提供同意書 (MTA, 非営利学術目的用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB02429 | pCAGhp53WT (Forward) | DNA solution |
icon below as well as clone information before placing your order.Materials & Methods section:
| The pCAGhp53WT (Forward) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02429). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB13743_A5Lop1-2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: pAxCA_F1 (Pr0164) Region: insert 5' Sequence file: RDB13743_A5Loa.seq
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>02429_13743_A5Lo_pAxCA_F1_A04_01.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NCCTTCTTCT TTTTNNNACN GCTCCTGGGN 61 AACGNGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTGATTTA TCGATTTAAT 121 TCTAGAGCCA CCGTCCAGGG NGCAGGTAGC TGCTGGGCTC CGGGGNCACT TTGCGTTCGG 181 GCTGGGAGCG TGCTTTCCAC GACGGTGACA CGCTTCCCTG GATTGGCAGC CAGACTGCCT 241 TCCGGGTCAC TGCCATGGAG GAGCCGCAGT CAGATCCTAG CGTCGAGCCC CCTCTGAGTC 301 AGGAAACATT TTCAGACCTA TGGAAACTAC TTCCTGAAAA CAACGTTCTG TCCCCCTTGC 361 CGTCCCAAGC AATGGATGAT TTGATGCTGT CCCCGGACGA TATTGAACAA TGGTTCACTG 421 AAGACCCAGG TCCAGATGAA GCTCCCAGAA TGCCAGAGGC TGCTCCCCCC GTGGCCCCTG 481 CACCAGCAGC TCCTACACCG GCGGCCCCTG CACCAGCCCC CTCCTGGCCC CTGTCATCTT 541 CTGTCCCTTC CCAGAAAACC TACCAGGGCA GCTACGGTTT CCGTCTGGGC TTCTTGCATT 601 CTGGGACAGC CAAGTCTGTG ACTTGCACGT ACTCCCCTGC CCTCAACAAG ATGTTTTGCC 661 AACTGGCCAA GACCTGCCCT GTGCAGCTGT GGGTTGANTC CACACCCCCG CCCGGCACCC 721 GCGTCCGCGC CATGGCCATC TACAAGCAGT CACAGCACAT GACGGANNNT GTGANGCGCT 781 GCCCCCACCA TGANCGCTGC TCANATAGCG ATGGTCTGGC CCCTCCTCAN CATCTTATCC 841 GANNGNNANN NAAATTNNCG NNNNNNNNAT TTGGNNGANA GNAACNCTTT TCNANATANN 901 GNNGNNNNNN NCCNANNANC CNNNNNNNNN N // |
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Primer: pCA-bgter (Pr0066) Region: insert 3' Sequence file: RDB13743_A5Lob.seq
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>02429_13743_A5Lo_pCA-bgter_H02_23.ab1 1 NNNNNNNNNN NNNNNNNNNT TTTGNNNNAN GGNNNNNNAN CTCNGTGGTA TTTGTGAGCC 61 AGGGCATTGG CCACACCAGC CACCACCTTC TGATAGGCAG CCTGCACCTG AGGAGTGAAT 121 TGATTATCGA TTCTAGACTA GTTTAATTAA TTAATTCCGG GACAAAGCAA ATGGAAGTCC 181 TGGGTGCTTC TGACGCACAC CTATTGCAAG CAAGGGTTCA AAGACCCAAA ACCCAAAATG 241 GCAGGGGAGG GAGAGATGGG GGTGGGAGGC TGTCAGTGGG GAACAAGAAG TGGAGAATGT 301 CAGTCTGAGT CAGGCCCTTC TGTCTTGAAC ATGAGTTTTT TATGGCGGGA GGTAGACTGA 361 CCCTTTTTGG ACTTCAGGTG GCTGGAGTGA GCCCTGCTCC CCCCTGGCTC CTTCCCAGCC 421 TGGGCATCCT TGAGTTCCAA GGCCTCATTC AGCTCTCGGA ACATCTCGAA GCGCTCACGC 481 CCACGGATCT GAAGGGTGAA ATATTCTCCA TCCAGTGGTT TCTTCTTTGG CTGGGGAGAG 541 GANCTGGTGT TGTTGGGCAN TGCTCGCTTA NTGCTCCCTG GGGGCAGCTC GTGGTGAGGC 601 TCCCCTTTCT TGCGGANATT CTCTTCCTCT GTGCGCCGGT CTCTCCCANG GANAGGCACN 661 NANANNCACC TCAAAGCTGT TCNNNNNNNN NN // |
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Primer: TP53-M_F (Pr1029) Region: insert mid(273aa,R) Sequence file: RDB13743_A5Loc.seq
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>02429_13743_A5Lo_TP53-M_F_F01_16_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNCN NTCNNCTACA ACTACNTGTG TAACAGTTCC TGCATGGGCG 61 GCATGAACCG GAGGCCCATC CTCACCATCA TCACACTGGA AGACTCCAGT GGTAATCTAC 121 TGGGACGGAA CAGCTTTGAG GTGCGTGTTT GTGCCTGTCC TGGGAGAGAC CGGCGCACAG 181 AGGAAGAGAA TCTCCGCAAG AAAGGGGAGC CTCACCACGA GCTGCCCCCA GGGAGCACTA 241 AGCGAGCACT GCCCAACAAC ACCAGCTCCT CTCCCCAGCC AAAGAAGAAA CCACTGGATG 301 GAGAATATTT CACCCTTCAG ATCCGTGGGC GTGAGCGCTT CGAGATGTTC CGAGAGCTGA 361 ATGAGGCCTT GGAACTCAAG GATGCCCAGG CTGGGAAGGA GCCAGGGGGG AGCAGGGCTC 421 ACTCCAGCCA CCTGAAGTCC AAAAAGGGTC AGTCTACCTC CCGCCATAAA AAACTCATGT 481 TCAAGACAGA AGGGCCTGAC TCAGACTGAC ATTCTCCACT TCTTGTTCCC CACTGACAGC 541 CTCCCACCCC CATCTCTCCC TCCCCTGCCA TTTTGGGTTT TGGGTCTTTG AACCCTTGCT 601 TGCNATANGT GTGCGTCAGA AGCACCCAGG ACTTCCATTT GCTTTGTCCN GGAATTAATT 661 AATTAAACTA GTCNANNNTC NANNATCNAN TCACTNNNNN NNGCNNNNTN NNNATCNNNN 721 NNNNNN // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
2024.03.26
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