Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAxCAhIFN-beta (forward)

Shuttle vector to produce rAd expressing human IFN-beta

Remarks, protocol and/or map (gif)	RDB02285.gif

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for not-for-profit research in the not-for-profit organization. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。 本件リソースは非営利機関にのみ提供し、非営利研究にのみ利用することができる。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14299_A6Htp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB14299_A6Hta.seq
>02285_14299_A6Ht_pAxCA_F1_F01_16_ABI24.ab1     1 TAAACGATCT TCTACATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC    61 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGAT TTATCGATTT GATCCGATTG   121 TCAACATGAC CAACAAGTGT CTCCTCCAAA TTGCTCTCCT GTTGTGCTTC TCCACTACAG   181 CTCTTTCCAT GAGCTACAAC TTGCTTGGAT TCCTACAAAG AAGCAGCAAT TTTCAGTGTC   241 AGAAGCTCCT GTGGCAATTG AATGGGAGGC TTGAATATTG CCTCAAGGAC AGGATGAACT   301 TTGACATCCC TGAGGAGATT AAGCAGCTGC AGCAGTTCCA GAAGGAGGAC GCCGCATTGA   361 CCATCTATGA GATGCTCCAG AACATCTTTG CTATTTTCAG ACAAGATTCA TCTAGCACTG   421 GCTGGAATGA GACTATTGTT GAGAACCTCC TGGCTAATGT CTATCATCAG ATAAACCATC   481 TGAAGACAGT CCTGGAAGAA AAACTGGAGA AAGAAGATTT CACCAGGGGA AAACTCATGA   541 GCAGTCTGCA CCTGAAAAGA TATTATGGGA GGATTCTGCA TTACCTGAAG GCCAAGGAGT   601 ACAGTCACTG TGCCTGGACC ATAGTCAGAG TGGAAATCCT AAGGAACTTT TACTTCATTA   661 ACAGACTTAC AGGTTACCTC CGAAACTGAA GATCTCCTAG CCTGAATCCA TATGACTAGT   721 AGATCCTCTA GAAATTAATT AAACTAGTCT AGAATCGATA ATCAATTCAC TCCTCAGGTG   781 CAGGCTGCCT ATCAGAAGGT GGTGGCTGGT GTGGCCAATG CCCTGGCTCA AAAATACCAC   841 TGAGATCTTT TTTCCCTCTG CCAAAAATTA TGGGGAACAT CATGAAGCCC CTTGAGCATC   901 TGACTTTCTG CTAATAAAAG AAAATTTATT ATTCATTGCT ATGGCAATAG TGGTGGTTGG   961 GAATTTTTTG GTGGTCCTCC TCACCTCGGG AAAGGACAAT ATGGGAAGGG GCCAAATCAA  1021 TTTAAAACAT CCAGAAATGG AGTATTTGGG TTTAGAGTTG GGCCAACAAT ATGCCCCAAT  1081 ATGCTGGGCG CGCCAAGGAA CAGGGGTGTC TATATAAAGA GAAGGTCATT CGAGTATATA  1141 TGGAAACAGC CCCCTTGCTT GTCCCATCCT TACTCCATAG AAAAGAGGCC CTTGGCACAC 1201 TGTGGAGAGT ATCCA //
Primer: Amp_R Sequence file: RDB14299_A6Htb.seq
>02285_14299_A6Ht_Amp_R_G01_19_ABI24.ab1     1 CTCGATTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG    61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC   121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA   181 GGCCCTTTCG TCTTCAAGAA TTCCGGATCC GGGCCAATAT GATAATGAGG GGGTGGAGTT   241 TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG   301 ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG   361 TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT   421 AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA   481 GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG   541 ACTTTGACCG TTTACGTGGA GACTCGCCCA GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC   601 GGGTCAAAGT TGGCGTTTTA TTATTATAGT CAGCATCGTT TAAACAGCTT GGGCCCTCGA   661 GGGGTCGAGG GATCTCCATA AGAGAAGAGG GACAGCTATG ACTGGGAGTA GTCAGGAGAG   721 GAGGAAAAAT CTGGCTAGTA AAACATGTAA GGAAAATTTT AGGGATGTTA AAGAAAAAAA   781 TAACACAAAA CAAAATATAA AAAAAATCTA ACCTCAAGTC AAGGCTTTTC CTATGGAATA   841 GGAATGGACA GCAGGGGGCT GTTTCATATA CTGATGACCT CTTTAATAGC CAACCTTTGT   901 TCATGGCAGC CAGCATATGG GCAATATGTT TGCCCAAACT CTAAACAAAT ACTCATCTGA   961 TGTTTAATGA ATTTGGCCCT CCAATATGTG TCTCGAGTGA AGACACAAAA TCACAACTAT  1021 TGTCATGAAG ATAAATCTTA TAGCTGAGTC GAGTCTACGG CCTCTGATGT GCCTAATTGC 1081 AAGGAAGATC GAGTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.10.12

GNP_filter3_RDBDEP_html_221010.pl