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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAxCAhFL

Shuttle vector to generate rAd expressing human FAS Ligand

Catalog number RDB02019
Resource name pAxCAhFL
Clone info. Shuttle vector to generate recombinant adenovirus Human Fas Ligand expression. cDNA fragment 195 to 1126nt of NM_000639.2 (CDS, 195 to 1040nt) is cloned.
Comment Use the COS-TPC method for production of adenovirus. Known difference: C to T substitution at 756nt of NM_000639.2 (Leu, synonym).
Vector backbone pAxcw (Cosmid, use packaging extracts for transformation)
Size of vector backbone 42.5 kb
Selectable markers Amp^r
Gene/insert name Human Fas ligand (FasL) cDNA
Depositor|Developer Hamada, Hirofumi |
Other clones in our bank

External Database
Human Fas ligand (FasL)

          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB02019.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Human Gene Therapy, 9, 2683-2689 (1998).) and an acknowledgment to the DEPOSITOR are requested.
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献 (Human Gene Therapy, 9, 2683-2689 (1998).) を引用し、謝辞の表明を必要とする。
          付加的提供条件:
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB02019 pAxCAhFL DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Shinoura, N., Construction, propagation, and titer estimation of recombinant adenoviruses carrying proapoptotic genes. Human Gene Therapy, 9, 2683-2689 (1998). PMID 9874266.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15036_A7Egp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Sequence file: RDB15036_A7Ega.seq check
          >02019_15036_A7Eg_pAxCA_F1_F05_17_ABI24.ab1
              1 TCAAACCATG GTTTTCCTTG GCCTTTCTTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT
             61 GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCGAT AAGCTTGCAT GCAGCAGCCC
            121 TTCAATTACC CATATCCCCA GATCTACTGG GTGGACAGCA GTGCCAGCTC TCCCTGGGCC
            181 CCTCCAGGCA CAGTTCTTCC CTGTCCAACC TCTGTGCCCA GAAGGCCTGG TCAAAGGAGG
            241 CCACCACCAC CACCGCCACC GCCACCACTA CCACCTCCGC CGCCGCCGCC ACCACTGCCT
            301 CCACTACCGC TGCCACCCCT GAAGAAGAGA GGGAACCACA GCACAGGCCT GTGTCTCCTT
            361 GTGATGTTTT TCATGGTTCT GGTTGCCTTG GTAGGATTGG GCCTGGGGAT GTTTCAGCTC
            421 TTCCACCTAC AGAAGGAGCT GGCAGAACTC CGAGAGTCTA CCAGCCAGAT GCACACAGCA
            481 TCATCTTTGG AGAAGCAAAT AGGCCACCCC AGTCCACCCC CTGAAAAAAA GGAGCTGAGG
            541 AAAGTGGCCC ATTTAACAGG CAAGTCCAAC TCAAGGTCCA TGCCTCTGGA ATGGGAAGAC
            601 ACCTATGGAA TTGTCCTGCT TTCTGGAGTG AAGTATAAGA AGGGTGGCCT TGTGATCAAT
            661 GAAACTGGGT TGTACTTTGT ATATTCCAAA GTATACTTCC GGGGTCAATC TTGCAACAAC
            721 CTGCCCCTGA GCCACAAGGT CTACATGAGG AACTCTAAGT ATCCCCAGGA TCTGGTGATG
            781 ATGGAGGGGA AGATGATGAG CTACTGCACT ACTGGGCAGA TGTGGGCCCG CAGCAGCTAC
            841 CTGGGGGGCA GTGTTCAATC TTACCAGTGC TGATCATTTA TATGTCAACG TATCTGAGCT
            901 CTCTCTGGTC AATTTTGAGG AATCTCAGAC GTTTTTCGGC TTATATAAGC TCTAAGAGAA
            961 GCACTTTGGA TTCTTTCCAT TATGATTCTT TGTTACAGGC ACCGAGAATG TTGTATTCGG
           1021 TGAGGTCTTC TTACATGCAG CCCGGGGGAT CCACTAGTTC TAGAATTCAC TCTCAAGGTG
           1081 CAGCCTGCCT ATTCAGAAGT GATGCTGGTG GTGCCCAAAT GCCTGGCTTC ACATACACTT
          1141 GAGATTCTTT TTCCCCTCTT GCCAAAAATT ATGGGGAACA TCTATGTGA
          //
          Primer: pAxCA_R1
          Sequence file: RDB15036_A7Egb.seq check
          >02019_15036_A7Eg_1_pAxCA_R1_F07_16_ABI24.ab1
              1 CAAGTCAACA AACGAACCCT TTCTAGATAA AGCAGCCTGC ACCTGAGGAG TGAATTCTAG
             61 AACTAGTGGA TCCCCCGGGC TGCATGTAAG AAGACCCTCA CCGAATACAA CATTCTCGGT
            121 GCCTGTAACA AAGAATCATA ATGGAAAGAA TCCCAAAGTG CTTCTCTTAG AGCTTATATA
            181 AGCCGAAAAA CGTCTGAGAT TCCTCAAAAT TGACCAGAGA GAGCTCAGAT ACGTTGACAT
            241 ATAAATGATC AGCACTGGTA AGATTGAACA CTGCCCCCAG GTAGCTGCTG CGGGCCCACA
            301 TCTGCCCAGT AGTGCAGTAG CTCATCATCT TCCCCTCCAT CATCACCAGA TCCTGGGGAT
            361 ACTTAGAGTT CCTCATGTAG ACCTTGTGGC TCAGGGGCAG GTTGTTGCAA GATTGACCCC
            421 GGAAGTATAC TTTGGAATAT ACAAAGTACA ACCCAGTTTC ATTGATCACA AGGCCACCCT
            481 TCTTATACTT CACTCCAGAA AGCAGGACAA TTCCATAGGT GTCTTCCCAT TCCAGAGGCA
            541 TGGACCTTGA GTTGGACTTG CCTGTTAAAT GGGCCACTTT CCTCAGCTCC TTTTTTTCAG
            601 GGGGTGGACT GGGGTGGCCT ATTTGCTTCT CCAAAGATGA TGCTGTGTGC ATCTGGCTGG
            661 TAGACTCTCG GAGTTCTGCC AGCTCCTTCT GTAGGTGGAA GAGCTGAAAC ATCCCCAGGC
            721 CCAATCCTAC CAAGGCAACC AGAACCATGA AAAACATCAC AAGGAGACAC AGGCCTGTGC
            781 TGTGGTTCCC TCTCTTCTTC AGGGGTGGCA GCGGTAGTGG AGGCAGTGGT GGCGGCGGCG
          841 GCGGAGGTGG TAGTGGTGGC GGTGGCGGTG GTGGTGGTGG CC
          //
          Primer: pAxcwit2-Fw
          Sequence file: RDB15036_A7Egc.seq check
          >02019_15036_A7Eg_1_pAxcwit2_Fw_D07_10_ABI24.ab1
              1 GCCCGCAGTG CCCTCCCCAC CACGTGGTCG GGGCCTCAGC TCCTCGGTCA CATCCAGCAT
             61 CACAGGCTGG TTCCTAATAT GTTTACCGCC ACACTCGCAG GGTCTGCACC TGGTGCGGGT
            121 CTCATCGTAC CTCAGCACCT TCCAGATCCA TCGTCGCGAG TCGACAATCG ATGTCGACAT
            181 TGATTATTGA CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT
            241 ATGGAGTTCC GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC
            301 CCCCGCCCAT TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC
            361 CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG
            421 TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT
            481 TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC
            541 ATCGCTATTA CCATGGTCGA GGTGAGCCCC ACGTTCTGCT TCACTCTCCC CATCTCCCCC
            601 CCCTCCCCAC CCCCAATTTT GTATTTATTT ATTTTTTAAT TATTTTGTGC AGCGATGGGG
          661 GCGGGGGGGG GGGGGGGGGG
          //
          Primer: Amp_R
          Sequence file: RDB15036_A7Egd.seq check
          >02019_15036_A7Eg_1_Amp_R_E07_13_ABI24.ab1
              1 TAGGGGGAAC CAGGCGTGAG GAGGCTTGTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
             61 ATTTGAATGT ATCCTATTAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
            121 GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT
            181 ATCACGAGGC CCTTTCGTCT TCAAGAATTC CGGATCCGGG CCAATATGAT AATGAGGGGG
            241 TGGAGTTTGT GACGTGGCGC GGGGCGTGGG AACGGGGCGG GTGACGTAGT AGTGTGGCGG
            301 AAGTGTGATG TTGCAAGTGT GGCGGAACAC ATGTAAGCGA CGGATGTGGC AAAAGTGACG
            361 TTTTTGGTGT GCGCCGGTGT ACACAGGAAG TGACAATTTT CGCGCGGTTT TAGGCGGATG
            421 TTGTAGTAAA TTTGGGCGTA ACCGAGTAAG ATTTGGCCAT TTTCGCGGGA AAACTGAATA
            481 AGAGGAAGTG AAATCTGAAT AATTTTGTGT TACTCATAGC GCGTAATATT TGTCTAGGGC
            541 CGCGGGGACT TTGACCGTTT ACGTGGAGAC TCGCCCAGGT GTTTTTCTCA GGTGTTTTCC
            601 GCGTTCCGGG TCAAAGTTGG CGTTTTATTA TTATAGTCAG CATCGATGAG CTTGATAGCT
            661 TGGGCTGCAG GTCGAGGGAT CTCCATAAGA GAAGAGGGAC AGCTATGACT GGGAGTAGTC
            721 AGGAGAGGAG GAAAAATCTG GCTAGTAAAA CATGTAAGGA AAATTTTAGG GATGTTAAAG
            781 AAAAAAATAA CACAAAACAA AATATAAAAA AAATCTAACC TCAAGTCAAG GCTTTTCTAT
            841 GGAATAAGGA ATGGACAGCA GGGGGCTGTT TCATATACTG ATGACCTCTT TATAGCCAAC
            901 CCTTTGTTCA TGGCAGCCAG CATATGGGCA TATGTTGCCC AAACTCTAAA CCAAATACTC
            961 ATTCTGATGT TTTAATGATT TGCCCTCCCA TATGTCCTTC CGAGTGAGAG ACAACAAAAA
           1021 ATTCCCAACA CACTAATTGC ATGAAAATAA AATTTCCCTT TTATTAGGCC AGAAGTCAGA
           1081 TGGCTTCAAG GGGGCTTCAT GATGTCCCCA TATTTTTTGG GCCAGAAGGG AAAGGATCCT
          1141 CAATTGTATT TGGTGGAGCA AGGGACAATT GGCCCACACA CGCAATCCAA CCATTGC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Shinoura, N., Construction, propagation, and titer estimation of recombinant adenoviruses carrying proapoptotic genes. Human Gene Therapy, 9, 2683-2689 (1998). PMID 9874266.

          2022.10.12

          GNP_filter3_RDBDEP_html_221010.pl