Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGMU20

E. coli - S. cerevisiae shuttle vector

Sequence	RDB01962z.seq
Remarks, protocol and/or map (gif)	RDB01962.gif
Remarks, protocol and/or map (gif)	RDB01962z.gif

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12316_A5Bg.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH_rev Sequence file: RDB12316_A5Bga.seq
>01962_12316_A5Bg_ADHrev_D08_11.ab1     1 TAAGATCTCA GAATAAGATT TTCGTTTTAA ACCTAAGAGT CACCCTCTAA AATTTGTATA    61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCCC   121 GGAATTAGCT TGGCTGCAGG TCGACTCACA GATCCTCTTC GGAAATCAGT TTCTGTTCAG   181 AATTCGAGCT CGGTACCCGG GGATCCTCTA GAGTCAATTC GATCCGGGGT TTTTTCTCCT   241 TGACGTTAAA GTATAGAGGT ATATTAACAA TTTTTTGTTG ATACTTTTAT TACATTTGAA   301 TAAGAAGTAA TACAAACCGA AAATGTTGAA AGTATTAGTT AAAGTGGTTA TGCAGTTTTT   361 GCATTTATAT ATCTGTTAAT AGATCAAAAA TCATCGCTTC GCTGATTAAT TACCCCAGAA   421 ATAAGGCTAA AAAACTAATC GCATTATCAT CCTATGGTTG TTAATTTGAT TCGTTCATTT   481 GAAGGTTTGT GGGGCCAGGT TACTGCCAAT TTTTCCTCTT CATAACCATA AAAGCTAGTA   541 TTGTAGAATC TTTATTGTTC GGAGCAGTGC GGCGCGAGGC ACATCTGCGT TTCAGGAACG   601 CGACCGGTGA AGACGAGGAC GCACGGAGGA GAGTCTTCCT TCGGAGGGCT GTCACCCGCT   661 CGGCGGCTTC TAATCCGTAC TTCAATATAG CAATGAGCAG TTAAGCGTAT TACTGAAAGT   721 TCCAAAGAGA AGGTTTTTTT AGGCTAAGAT AATGGGGCTC TTTACATTTC CACAACATAT   781 AAGTAAGATT AGATATGGAT ATGTATATGG ATATGTATAT GGTGGTAATG CCATGTAATA   841 TGATTATTAA ACTTCTTTGC GTCCATCCAA AAAAAAAGTA AGAATTTTTT GAAAATTCAA   901 TATAAATGAC AGCTCAGTTA CAAAGTGAAA GTGACCTGCA GGCATGCAAG CAACGAAGCA   961 TCTGTGCTTC ATTTTGTAGA ACAAAAATGC AACGCGAGAG CGCTAAATTT TTCAAACAAA  1021 GAATCTGAGC TGCATTTTTT TACAGACAGA AATGCACGCG GAAGCGCTAT TTTACAACGA 1081 AGAATCTGTG CTCAATTTTT GGTAAAACCA AAAAAGTTGC AACCGCCG //
Primer: Amp_R Sequence file: RDB12316_A5Bgb.seq
>01962_12316_A5Bg_Amp_R_E08_14.ab1     1 TCTATTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT    61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA   121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG   181 AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC   241 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC   301 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT   361 GTACTGAGAG TGCACCATAA CGCATTTAAG CATAAACACG CACTATGCCG TTCTTCTCAT   421 GTATATATAT ATACAGGCAA CACGCAGATA TAGGTGCGAC GTGAACAGTG AGCTGTATGT   481 GCGCAGCTCG CGTTGCATTT TCGGAAGCGC TCGTTTTCGG AAACGCTTTG AAGTTCCTAT   541 TCCGAAGTTC CTATTCTCTA GCTAGAAAGT ATAGGAACTT CAGAGCGCTT TTGAAAACCA   601 AAAGCGCTCT GAAGACGCAC TTTCAAAAAA CCAAAAACGC ACCGGACTGT AACGAGCTAC   661 TAAAATATTG CGAATACCGC TTCCACAAAC ATTGCTCAAA AGTATCTCTT TGCTATATAT   721 CTCTGTGCTA TATCCCTATA TAACCTACCC ATCCACCTTT CGCTCCTTGA ACTTGCATCT   781 AAACTCGACC TCTACATTTT TTATGTTTAT CTCTAGTATT ACTCTTTAGA CAAAAAAAAT   841 TGTAGTAAGA ACTATTCATA GAGTGAATCG AAAACAATAC GAAAAATGTA AACATTTCCT   901 ATACGTAGTA TATAGAGACA AAATAGAAGA AACCGTTCAT AATTTTCTGA CCAATGAAGA   961 ATCATCAACG CTATCACTTT CTGTTCACAA AGTATGCGCA ATCCACATCG GTATAGAAAT  1021 ATAAATCGGG GATGCCCTTT ATCTGAAAAA ATGCACCCCG CAGCCTTCGC CTAGTAATCA  1081 GTAAACCGCG GGCAGTTGCA AGTTCAAGCC TTTTTTTATG GCCAGCAGCA AAAAATTAGA 1141 CCACCCAAAA AAAG //
Primer: URA3pro_F Sequence file: RDB12316_A5Bgc.seq
>01962_12316_A5Bg_URA3pro_F_F08_17.ab1     1 CAATGCTGTG CTGAGACATG AATTGCCCAG TATTCTTAAC CCAACTGCAC AGAACAAAAA    61 CCTGCAGGAA ACGAAGATAA ATCATGTCGA AAGCTACATA TAAGGAACGT GCTGCTACTC   121 ATCCTAGTCC TGTTGCTGCC AAGCTATTTA ATATCATGCA CGAAAAGCAA ACAAACTTGT   181 GTGCTTCATT GGATGTTCGT ACCACCAAGG AATTACTGGA GTTAGTTGAA GCATTAGGTC   241 CCAAAATTTG TTTACTAAAA ACACATGTGG ATATCTTGAC TGATTTTTCC ATGGAGGGCA   301 CAGTTAAGCC GCTAAAGGCA TTATCCGCCA AGTACAATTT TTTACTCTTC GAAGACAGAA   361 AATTTGCTGA CATTGGTAAT ACAGTCAAAT TGCAGTACTC TGCGGGTGTA TACAGAATAG   421 CAGAATGGGC AGACATTACG AATGCACACG GTGTGGTGGG CCCAGGTATT GTTAGCGGTT   481 TGAAGCAGGC GGCAGAAGAA GTAACAAAGG AACCTAGAGG CCTTTTGATG TTAGCAGAAT   541 TGTCATGCAA GGGCTCCCTA TCTACTGGAG AATATACTAA GGGTACTGTT GACATTGCGA   601 AGAGCGACAA AGATTTTGTT ATCGGCTTTA TTGCTCAAAG AGACATGGGT GGAAGAGATG   661 AAGGTTACGA TTGGTTGATT ATGACACCCG GTGTGGGTTT AGATGACAAG GGAGACGCAT   721 TGGGTCAACA GTATAGAACC GTGGATGATG TGGTCTCTAC AGGATCTGAC ATTATTATTG   781 TTGGAAGAGG ACTATTTGCA AAGGGAAGGG ATGCTAAGGT AGAGGGTGAA CGTTACAGAA   841 AAGCAGGCTG GGAAGCATAT TTGAGAAGAT GCGGCCAGCA AAACTAAAAA AACTGTATTA   901 TAAGTAAAAG CTTACATTTT ATGTTAGCTG GTGGACTGAC GCCAGAAAAT GTTGGTGATG   961 CGCTTAGATT AAATGGGCGT TTATTGGTGT TTGATGTAAG CGGGAGGTGT GGAGACAAAT  1021 GGTGTAAAAG ACTCTACAAA ATAGCAATTT CGTCAAAAAT GCTAGAAATA GTATTACTGA 1081 GTAGTATTAT TAGGTATTGA TTGTTGCACT TGTCCGGCTA GAAGGGTG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.18

GNP_filter3_RDBDEP_html_230702.pl