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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAxCAmIL12p35

Shuttle vector to generate rAd expressing mouse IL-12 p35 cDNA

Catalog number RDB01413
Resource name pAxCAmIL12p35
Alternative name pAdex1CAmIL12p35
Clone info. Shuttle vector to generate recombinant adenovirus vector expressing mouse IL-12p35 gene.
Comment Produce adenovirus by COS-TPC method.
Vector backbone pAxCAwt (Cosmid, use packaging extracts for transformation)
Size of vector backbone 44,784 bp
Selectable markers Amp^r
Gene/insert name Mouse IL-12 p35 cDNA
Depositor|Developer Hamada, Hirofumi |
Other clones in our bank
External Database
Mouse IL-12 p35
          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB01413.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
          付加的提供条件：
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB01413 pAxCAmIL12p35 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pAxCAmIL12p35 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01413).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB01413_B2L1p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1 (Pr0164)
          Region: insert 5'
          Sequence file: RDB01413_B2L1a.seq check
          >01413_01413_B2L1_3_pAxCA_F1_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNTG CCTTCTTCTT TTTCCTACNG CTCCTGGGCA ACGTGCTGGT
             61 TATTGTGCTG TCTCATCATT TTGGCAAAGC TCACCCTTAC CGAGTCGGCG ACACAGTGTG
            121 GGTCCGCCGA CACCAGACTA AGAACCTAGA ACCTCGCTGG AAAGGACCTT ACACAGTCCT
            181 GCTGACCACC CCCACCGCCC TCAAAGTAGA CGGCATCGCA GCTTGGATAC ACGCCGCCCA
            241 CGTGAAGGCT GCCGACCCCG GGGGTGGACC ATCCTCTAGA CTGCCATGGG ATGTCAATCA
            301 CGCTACCTCC TCTTTTTGGC CACCCTTGCC CTCCTAAACC ACCTCAGTTT GGCCAGGGTC
            361 ATTCCAGTCT CTGGACCTGC CAGGTGTCTT AGCCAGTCCC GAAACCTGCT GAAGACCACA
            421 GATGACATGG TGAAGACGGC CAGAGAAAAA CTGAAACATT ATTCCTGCAC TGCTGAAGAC
            481 ATCGATCATG AAGACATCAC ACGGGACCAA ACCAGCACAT TGAAGACCTG TTTACCACTG
            541 GAACTACACA AGAACGAGAG TTGCCTGGCT ACTAGAGAGA CTTCTTCCAC AACAAGAGGG
            601 AGCTGCCTGC CCCCACAGAA GACGTCTTTG ATGATGACCC TGTGCCTTGG TAGCATCTAT
            661 GAGGACTTGA AGANGTACCA GACAGAGTTC CAGGCCATCA ACGCAGCACT TCAGAATCAC
            721 AACCATCAGC AGATCATTCT AGACAAGGGC ATGCTGGTGG CCATCGATGA NCTGATGCAG
            781 TCTCTGAATC ATAATGGCGA GACTCTGCGC CAGAAACCTC CTGTGGGANA AGCANANCCT
            841 TACAGANTGA AAATGAAGCT CTGCATCCTG CTTCACGCCT NCANCACCCG CNNCNNGNNC
            901 NTCNNCAGGG NGATGGNNTA TCTGANCTCC GCNNNNNNAT CNGGANTANN CCNANTTNGN
          961 NNAANANNNN NTCNNNTCAC NNCNNNNNNN NNN
          //
          Primer: pAxCA_R1 (Pr0165)
          Region: insert 3'
          Sequence file: RDB01413_B2L1b.seq check
          >01413_01413_B2L1_3_pAxCA_R1_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NCNAGCNNCN NCCTTCTGNT AGGCAGCCTG CACCTGANGN
             61 GTGAATTGAT CCTGTCTTTA ACAAATTGGA CTAATCCGGA TCCTCAGGCG GAGCTCAGAT
            121 AGCCCATCAC CCTGTTGATG GTCACGACGC GGGTGCTGAA GGCGTGAAGC AGGATGCAGA
            181 GCTTCATTTT CACTCTGTAA GGGTCTGCTT CTCCCACAGG AGGTTTCTGG CGCAGAGTCT
            241 CGCCATTATG ATTCAGAGAC TGCATCAGCT CATCGATGGC CACCAGCATG CCCTTGTCTA
            301 GAATGATCTG CTGATGGTTG TGATTCTGAA GTGCTGCGTT GATGGCCTGG AACTCTGTCT
            361 GGTACATCTT CAAGTCCTCA TAGATGCTAC CAAGGCACAG GGTCATCATC AAAGACGTCT
            421 TCTGTGGGGG CAGGCAGCTC CCTCTTGTTG TGGAAGAAGT CTCTCTAGTA GCCAGGCAAC
            481 TCTCGTTCTT GTGTAGTTCC AGTGGTAAAC AGGTCTTCAA TGTGCTGGTT TGGTCCCGTG
            541 TGATGTCTTC ATGATCGATG TCTTCAGCAG TGCAGGAATA ATGTTTCAGT TTTTCTCTGG
            601 CCGTCTTCAC CATGTCATCT GTGGTCTTCA GCAGGTTTCG GGACTGGCTA AGACACCTGG
            661 CAGGTCCAGA GACTGGAATG ACCCTGGCCA AACTGAGGTG GTTTAGGAGG GCAAGGGTGG
            721 CCAAAAAGAG GANGTAGCGT GATTGACATC CCATGGCAGT CTAGAGGATG GTCCACCCCN
            781 GGGGTCGGCA GCCTTCACGT GNNCGGCGTG TATCCNAGCT GCNATGCCGT CTACTTTGAN
            841 GGCGGNGGGG GTGGNCAGCA NGANTGTGTA ANGNCCTTNN CAGCGAGGTT NTANGTTCTT
            901 ANNCTGGNGT CGGNNGANCC NCACTGNGTC GNNNANTCNG NNNNGNNNNN CTTTGNNNAA
          961 ANGANGANNN NNNNNNN
          //
          Primer: Amp_R (Pr0008)
          Region: AmpR pro,ITR
          Sequence file: RDB01413_B2L1c.seq check
          >01413_01413_B2L1_3_Amp_R_C01_07_ABI24.ab1
              1 NNNNNNNNNN ANNANTNANC NNTTNNNNGG GTTNNTNNCT CNTGAGCGGA NACATATTTG
             61 AATGNATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
            121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
            181 GGCCCTTTCG TCTTCAAGAA TTCCGGATCC GGGCCNATAT GATAATGANG GGGTGGANTT
            241 TGTGACGNGG CGCGGGGNGN GGGAACGGGG NGGNNGNNGN ANNANNGNGG NNNAANNNNG
          301 ANGNTNCANN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl