RDB01273 - pSV2 erb B2

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pSV2 erb B2

Human erb B2 cDNA expression vector

Catalog number	RDB01273
Resource name	pSV2 erb B2
Alternative name	Human erb B2 cDNA expression vector
Clone info.	Human erb B2 cDNA driven by SV40 promoter
Vector backbone	pSV2 (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human c-erbB2/erb-B cDNA
Depositor\|Developer	Yamamoto, Tadashi \|

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human c-erbB2/erb-B

External Database
Human c-erbB2/erb-B

Reference sequence

Remarks, protocol and/or map (gif)	RDB01273.gif

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01273	pSV2 erb B2	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pSV2 erb B2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01273).

Reference section:

Masuko, T., Sugahara, K., Kozono, M., Otsuki, S., Akiyama, T., Yamamoto, T., Toyoshima, K., Hashimoto, Y., A murine monoclonal antibody that recognizes an extracellular domain of the human c-erbB-2 protooncogene product. Jpn. J. Cancer Res. 80 (1): 10-14 (1989).original PMID 2565325.

Further references such as user reports and related articles (go to bottom)

References and tips

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13019_A4Khp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pro_F () Region: SV40pro_ori,insert5' Sequence file: RDB13019_A4Kha.seq
>01273_13019_A4Kh_SV40pro_F_A05_02.ab1 1 GGCTTGGCCT TTACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA 61 AAAGCTTGGG GCAGCCGCGC GCCCCTTCCC ACGGGGCCCT TTACTGCGCC GCGCGCCCGG 121 CCCCCACCCC TCGCAGCACC CCGCGCCCCG CGCCCTCCCA GCCGGGTCCA GCCGGAGCCA 181 TGGGGCCGGA GCCGCAGTGA GCACCATGGA GCTGGCGGCC TTGTGCCGCT GGGGGCTCCT 241 CCTCGCCCTC TTGCCCCCCG GAGCCGCGAG CACCCAAGTG TGCACCGGCA CAGACATGAA 301 GCTGCGGCTC CCTGCCAGTC CCGAGACCCA CCTGGACATG CTCCGCCACC TCTACCAGGG 361 CTGCCAGGTG GTGCAGGGAA ACCTGGAACT CACCTACCTG CCCACCAATG CCAGCCTGTC 421 CTTCCTGCAG GATATCCAGG AGGTGCAGGG CTACGTGCTC ATCGCTCACA ACCAAGTGAG 481 GCAGGTCCCA CTGCAGAGGC TGCGGATTGT GCGAGGCACC CAGCTCTTTG AGGACAACTA 541 TGCCCTGGCC GTGCTAGACA ATGGAGACCC GCTGAACAAT ACCACCCCTG TCACAGGGGC 601 CTCCCCAGGA GGCCTGCGGG AGCTGCAGCT TCGAAGCCTC ACAGAGATCT TGAAAGGAGG 661 GGTCTTGATC CAGCGGAACC CCCAGCTCTG CTACCAGGAC ACGATTTTGT GGAAGGACAT 721 CTTCCACAAG AACAACCAGC TGGCTCTCAC ACTGATAGAC ACCAACCGCT CTCGGGCCTG 781 CCACCCCTGT TCTCCGATGT GTAAGGGCTC CCGGTGCTGG GGAGAGAGTT CTGAGGATTG 841 TCAGAGCCTG ACGCGCACTG TCTGTGCCGG TGGCTGTGCC CGCTGCAAGG GGCCACTGCC 901 CACTGACTGC TGCCATGAGC AGTGTGCTGC CGGCTGCACG GGCCCCAAGC ACTCTGACTG 961 CCTGGCCTGC CTCCACTTCA ACCACAGTGG CATCTGTGAG CTGCACTGCC CAGCCCTGGG 1021 TCACCTACAA CACAGACACG TTTTGAGTCC ATGCCCAAAT CCCGAGGGCC CGGGTAATAC 1081 ATTTCGGCGC CAGCTGTGTG AACTGCCCTG GTCCTTACAC TAACCTTTCC TACCGGAACG 1141 TGGGAATCCT GCACCCCTTC CGGTTCCTTG CCCCCCCCCT TGTC //
Primer: SV40-3'UTR () Region: insert3' Sequence file: RDB13019_A4Khb.seq
>01273_13019_A4Kh_SV40-3'UTR_A05_02.ab1 1 TCAACAGCAA GGTAGGTTCC TTCACAAAGA TCCAAGCTTG CAAGCTTGAA AAAAACAAAA 61 CAAAAAAAGT AAAAACTAAA CAGAAAAGCA CTCTGTACAA AGCCTGGATA CTGACACCAT 121 TGCTGTTCCT TCCTCATGGG GGGCAGTACT AGGTTTCAGG GACAGTCTCT GAATGGGTCG 181 CTTTTGTTCT TAGACACTCC CTTAGGGCCG CTTCCCCTCT CAGGCCAGCT TCCCTAAGGC 241 TTTCAGTACC CAGGATCTGG AAGGAAAGGG CCAAGCTGGA CTGTGGCATC CACTGGACCC 301 TAGAGTCTCA TTGGGCAGGG CCTCAGAATC CACAAAGACT CCCCAGTGCT GTTCCTCTTC 361 CAACGAGGCT GGACCCCTTC CAGCCATCTG GGAACTCAAG CAGGAAGGAA GGTTCCTTAG 421 GACAGGTTCC TGGCATGGCA GGTTCCCCTG GAAGTGGTCG GAGGGCCCTC CCACCTCTTG 481 ATGCCAGCAG AAGTCAGGCC TTCCCTGCTC CCTGAGGACA CATCAGGGCT TCTGCGGACT 541 TGGCCTTCTG GTTCACACTG GCACGTCCAG ACCCAGGTAC TCTGGGTTCT CTGCCGTAGG 601 TGTCCCTTTG AAGGTGCTGG GTGGAGCCCC CCGCTCTGGT GGGTCCTGGT CCCAGTAATA 661 GAGGTTGTCG AAGGCTGGGC TGAAGGCAGG AGGAGGGTGG GGCTGAGGGG CAGCTCCTCC 721 CTGGGGTGTC AAGTACTCGG GGTTCTCCAC GGCACCCCCA AAGGCAAAAA CGTCTTTGAC 781 GACCCCATTC TTCCCTGGGG AGAGAGTCTT GGGCCTTTCC AGAGTGGCAC CAGCAGGTCG 841 GGCAGCAGGC AGAGGGCCCT CTCGGGGCGA AGGGGGCTGG GGCCGAACAT CTGGCTGGTT 901 CACATATTCA GGCTGGGGGC TGCAGGTCAG GGGGGCAACG TAGCCATCAG TCTCAGAGGG 961 CAGGGGTACT GTGGGTCCTC ACTGTACCGC TGTAGAGGCT GGGGTCATGT GTGGGGAGGC 1021 TTTGCAGCCC CTTGGCTGCC CCATTCCCAG GTCACCATCA ATACATCGGA GCCAGCCCCT 1081 TTCGGAGGTG CCAGTGAGAC CTGGGGCCTC CTCACAGAGG GCTCAGCCTA GTGTCAGGTT 1141 CCCCACGACC ACTCCTTGGT TAGAAATGAA GCCTTGTCCG //
Primer: pAxCALNLF1 () Region: SV40p(A) Sequence file: RDB13019_A4Khc.seq
>01273_13019_A4Kh_pAxCALNL_F1_A12_03.ab1 1 ACAATTTTTT CCTTAATTCA TGTTCTGGGA TCCCCAGGAA GCTCCTCTGT GTCCTCATAA 61 ACCCTAACCT CCTCTACTTG AGAGGACATT CCAATCATAG GCTGCCCATC CACCCTCTGT 121 GTCCTCCTGT TAATTAGGTC ACTTAACAAA AAGGAAATTG GGTAGGGGTT TTTCACAGAC 181 CGCTTTCTAA GGGTAATTTT AAAATATCTG GGAAGTCCCT TCCACTGCTG TGTTCCAGAA 241 GTGTTGGTAA ACAGCCCACA AATGTCAACA GCAGAAACAT ACAAGCTGTC AGCTTTGCAC 301 AAGGGCCCAA CACCCTGCTC ATCAAGAAGC ACTGTGGTTG CTGTGTTAGT AATGTGCAAA 361 ACAGGAGGCA CATTTTCCCC ACCTGTGTAG GTTCCAAAAT ATCTAGTGTT TTCATTTTTA 421 CTTGGATCAG GAACCCAGCA CTCCACTGGA TAAGCATTAT CCTTATCCAA AACAGCCTTG 481 TGGTCAGTGT TCATCTGCTG ACTGTCAACT GTAGCATTTT TTGGGGTTAC AGTTTGAGCA 541 GGATATTTGG TCCTGTAGTT TGCTAACACA CCCTGCAGCT CCAAAGGTTC CCCACCAACA 601 GCAAAAAAAT GAAAATTTGA CCCTTGAATG GGTTTTCCAG CACCATTTTC ATGAGTTTTT 661 TGTGTCCCTG AATGCAAGTT TAACATAGCA GTTACCCCAA TAACCTCAGT TTTAACAGTA 721 AACAGCTTCC CACATCAAAA TATTTCCACA GGTTAAAGTC CTCATTTAAA TTAGGCAAAG 781 GAATTCTTGA AAGACGAAAG GGCCCTCGTG ATACGCCTAT TTTTATAGGT TTATGTCATG 841 ATAATAATGG TTTCTTAAAC GTCAGGTGGC ACCTTTTCGG GGGAAAATGT GCGCGGAACC 901 CCTATCTTGT ATATTTTTTC TAAAAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Masuko, T., A murine monoclonal antibody that recognizes an extracellular domain of the human c-erbB-2 protooncogene product. Jpn. J. Cancer Res. 80 (1): 10-14 (1989).original PMID 2565325.
reference	Matsuda, S., 17 beta-estradiol mimics ligand activity of the c-erbB2 protooncogene product. Proc. Natl. Acad. Sci. U.S.A. 90 (22): 10803-10807 (1993). PMID 7902571.
reference	Ullrich, A., Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells. Nature 319, 230-234 (1986). PMID 6328312.
user_report	Miura, T., Ligand-inducible dimeric antibody for selecting antibodies against a membrane protein based on mammalian cell proliferation. Biotechnol. Bioeng. 113 (5): 1113-1123 (2016). PMID 26479395.

2023.05.02

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