Lectin

Galectin

A note on expression and purification of recombinant galectins.

Nishi, N. Glycoforum 23 (5), A15 (2020). DOI: https://doi.org/10.32285/glycoforum.23A15

Galectin-1

Catalog no. Name of resource Vector Running title
RDB04217 pGEX-G1-1a pGEX-4T-2 Bacteria expression vector of human galectin-1, GST fusion protein
RDB08392 pET-G1-1a pET-11a Bacteria expression vector of human galectin-1, tag-free protein
RDB08448 pGEX-CSG1-2a pGEX-4T-2 Bacteria expression vector of human galectin-1 in which all Cys residues are substituted with Ser residues (G1C3,17,43,61,89,131S: CSG1), GST fusion protein
RDB08449 pET-CSG1-1a pET-11a Bacteria expression vector of human galectin-1 in which all Cys residues are substituted with Ser residues (G1C3,17,43,61,89,131S: CSG1), tag-free protein
RDB08433 pGEX-mG1-1a pGEX-4T-2 Bacteria expression vector of mouse galectin-1, GST fusion protein
RDB08434 pET-mG1-1a pET-11a Bacteria expression vector of mouse galectin-1, tag-free protein

Galectin-2

Catalog no. Name of resource Vector Running title
RDB04218 pGEX-G2-1a pGEX-4T-2 Bacteria expression vector of human galectin-2, GST fusion protein
RDB08435 pGEX-mG2-1a pGEX-4T-2 Bacteria expression vector of mouse galectin-2, GST fusion protein

Galectin-3

Catalog no. Name of resource Vector Running title
RDB04219 pGEX-G3-1a pGEX-4T-2 Bacteria expression vector of human galectin-3, GST fusion protein
RDB08393 pET-G3-1a pET-11a Bacteria expression vector of human galectin-3, tag-free protein
RDB08394 pGEX-G3CRD-1a pGEX-4T-2 Bacteria expression vector of human galectin-3 (C-terminal lectin domain/carbohydrate recognition domain, G3CRD), GST fusion protein
RDB08395 pET-G3CRD-1a pET-11a Bacteria expression vector of human galectin-3 (C-terminal lectin domain/carbohydrate recognition domain, G3CRD), tag-free protein
RDB08436 pGEX-mG3-3a pGEX-4T-2 Bacteria expression vector of mouse galectin-3, GST fusion protein

Galectin-4

Catalog no. Name of resource Vector Running title
RDB08396 pGEX-G4-1a pGEX-4T-2 Bacteria expression vector of human galectin-4, GST fusion protein
RDB04220 pTrc-G4-1a pTrc-HisB Bacteria expression vector of human galectin-4, (His) 6 -containing-tag protein
RDB18238 pGEX-G4Null pGEX-4T-2 Bacteria expression vector of human galectin-4 with a truncated linker peptide (G4Null), GST fusion protein
RDB18237 pGEX-G4NT pGEX-4T-2 Bacteria expression vector of human galectin-4 (N-terminal CRD with a part of the linker peptide, G4NT), GST fusion protein
RDB08398 pGEX-G4CT-1a pGEX-4T-2 Bacteria expression vector of human galectin-4 (C-terminal CRD with a part of the linker peptide, G4CT), GST fusion protein
RDB08437 pGEX-mG4-1a pGEX-4T-2 Bacteria expression vector of mouse galectin-4, GST fusion protein

Galectin-7

Catalog no. Name of resource Vector Running title
RDB04221 pGEX-G7-5a pGEX-4T-2 Bacteria expression vector of human galectin-7, GST fusion protein
RDB08399 pET-G7-1a pET-11a Bacteria expression vector of human galectin-7, tag-free protein
RDB08400 pGEX-G7R54H-12a pGEX-4T-2 Bacteria expression vector of human galectin-7 with an R54H mutation, GST fusion protein
RDB08438 pGEX-mG7-7a pGEX-4T-2 Bacteria expression vector of mouse galectin-7, GST fusion protein
RDB08439 pET-mG7-1a pET-11a Bacteria expression vector of mouse galectin-7, tag-free protein

Galectin-8

Catalog no. Name of resource Vector Running title
RDB04222 pGEX-G8(M)-2a pGEX-4T-2 Bacteria expression vector of human galectin-8 (the predominant isoform of G8 in most cell and tissue types, G8(M)/G8M), GST fusion protein
RDB08404 pET-G8(M)-1a pET-11a Bacteria expression vector of human galectin-8 (the predominant isoform of G8 in most cell and tissue types, G8(M)/G8M), tag-free protein
RDB08405 pGEX-G8(L)-1a pGEX-4T-2 Bacteria expression vector of human galectin-8 with a long linker peptide (G8(L)/G8L), GST fusion protein
RDB08410 pET-G8(L)-12a pET-11a Bacteria expression vector of human galectin-8 with a long linker peptide (G8(L)/G8L), tag-free protein
RDB08401 pGEX-G8(M)R69H-1a pGEX-4T-2 Bacteria expression vector of human galectin-8 (the predominant isoform of G8 in most cell and tissue types, G8(M)/G8M) with an R69H mutation, GST fusion protein
RDB08402 pGEX-G8(M)R233H-2a pGEX-4T-2 Bacteria expression vector of human galectin-8 (the predominant isoform of G8 in most cell and tissue types, G8(M)/G8M) with an R233H mutation, GST fusion protein
RDB08403 pGEX-G8(M)R69,233H-1a pGEX-4T-2 Bacteria expression vector of human galectin-8 (the predominant isoform of G8 in most cell and tissue types, G8(M)/G8M) with R69,233H mutations, GST fusion protein
RDB08454 pGEX-G8Null-1a pGEX-4T-2 Bacteria expression vector of human galectin-8 with a truncated linker peptide (G8Null), GST fusion protein
RDB08455 pET-G8Null-5a pET-11a Bacteria expression vector of human galectin-8 with a truncated linker peptide (G8Null), tag-free protein
RDB08411 pGEX-G8NT-1a pGEX-4T-2 Bacteria expression vector of human galectin-8 (N-terminal CRD with a part of the linker peptide, G8NT), GST fusion protein
RDB08412 pGEX-G8CT-1a pGEX-4T-2 Bacteria expression vector of human galectin-8 (C-terminal CRD with a part of the linker peptide, G8CT), GST fusion protein
RDB08406 pGEX-G8(L)R69H-1a pGEX-4T-2 Bacteria expression vector of human galectin-8 with a long linker peptide (G8(L)/G8L) with an R69H mutation, GST fusion protein
RDB08407 pGEX-G8(L)R275H-3a pGEX-4T-2 Bacteria expression vector of human galectin-8 with a long linker peptide (G8(L)/G8L) with an R275H mutation, GST fusion protein
RDB08408 pGEX-G8(L)R69,275H-1a pGEX-4T-2 Bacteria expression vector of human galectin-8 with a long linker peptide (G8(L)/G8L) with R69,275H mutations, GST fusion protein
RDB08409 pGEX-G8(L)R197A-1a pGEX-4T-2 Bacteria expression vector of human galectin-8 with a long linker peptide (G8(L)/G8L) with an R197A mutation, GST fusion protein
RDB08413 pGEX-G8NCRD-1a pGEX-4T-2 Bacteria expression vector of human galectin-8 (N-terminal CRD, G8NCRD), GST fusion protein
RDB08414 pGEX-G8CCRD-1a pGEX-4T-2 Bacteria expression vector of human galectin-8 (C-terminal CRD, G8CCRD), GST fusion protein
RDB08440 pGEX-mG8(M)-2a pGEX-4T-2 Bacteria expression vector of mouse galectin-8 (the predominant isoform of mG8 in most cell and tissue types, mG8(M)/mG8M), GST fusion protein
RDB08441 pET-mG8(M)-1a pET-11a Bacteria expression vector of mouse galectin-8 (the predominant isoform of mG8 in most cell and tissue types, mG8(M)/mG8M), tag-free protein
RDB08456 pET-mG8Null-2a pET-11a Bacteria expression vector of mouse galectin-8 with a truncated linker peptide (mG8Null), tag-free protein
RDB08445 pGEX-RaG8(M)-1a pGEX-4T-2 Bacteria expression vector of rat galectin-8 (the predominant isoform of rat G8 in most cell and tissue types, RaG8(M)/RaG8M), GST fusion protein
RDB08446 pET-RaG8(M)-1a pET-11a Bacteria expression vector of rat galectin-8 (the predominant isoform of rat G8 in most cell and tissue types, RaG8(M)/RaG8M), tag-free protein

Galectin-9

Catalog no. Name of resource Vector Running title
RDB08415 pGEX-G9(S)-1a pGEX-4T-2 Bacteria expression vector of human galectin-9 with a short linker peptide (G9(S)/G9S), GST fusion protein
RDB08416 pET-G9(S)-3a pET-11a Bacteria expression vector of human galectin-9 with a short linker peptide (G9(S)/G9S), tag-free protein
RDB04223 pGEX-G9(M)-1a pGEX-4T-2 Bacteria expression vector of human galectin-9 (the predominant isoform of G9 in most cell and tissue types, G9(M)/G9M), GST fusion protein
RDB08420 pET-G9(M)-4a pET-11a Bacteria expression vector of human galectin-9 (the predominant isoform of G9 in most cell and tissue types, G9(M)/G9M), tag-free protein
RDB08421 pGEX-G9(L)-2a pGEX-4T-2 Bacteria expression vector of human galectin-9 with a long linker peptide (G9(L)/G9L), GST fusion protein
RDB08422 pET-G9(L)-5a pET-11a Bacteria expression vector of human galectin-9 with a long linker peptide (G9(L)/G9L), tag-free protein
RDB08417 pGEX-G9(M)R65H-2a pGEX-4T-2 Bacteria expression vector of human galectin-9 (the predominant isoform of G9 in most cell and tissue types, G9(M)/G9M) with an R65H mutation, GST fusion protein
RDB08418 pGEX-G9(M)R239H-1a pGEX-4T-2 Bacteria expression vector of human galectin-9 (the predominant isoform of G9 in most cell and tissue types, G9(M)/G9M) with an R239H mutation, GST fusion protein
RDB08419 pGEX-G9(M)R65,239H-1a pGEX-4T-2 Bacteria expression vector of human galectin-9 (the predominant isoform of G9 in most cell and tissue types, G9(M)/G9M) with R65,239H mutations, GST fusion protein
RDB08451 pET-G9Null-11a pET-11a Bacteria expression vector of human galectin-9 with a truncated linker peptide (G9Null), tag-free protein
RDB08452 pET-G9NullR65D-2a pET-11a Bacteria expression vector of human galectin-9 with a truncated linker peptide (G9Null) with an R65D mutation, tag-free protein
RDB08453 pET-G9NullR212D-3a pET-11a Bacteria expression vector of human galectin-9 with a truncated linker peptide (G9Null) with an R212D mutation, tag-free protein
RDB15282 pET-ssG9 pET-11a Bacteria expression vector of human galectin-9 with a truncated linker peptide (G9Null) with a 10-amino acid deletion and a single amino acid mutation at the remaining linker peptide (ssG9), tag-free protein
RDB08423 pGEX-G9NT-1a pGEX-4T-2 Bacteria expression vector of human galectin-9 (N-terminal CRD with a part of the linker peptide, G9NT), GST fusion protein
RDB08424 pGEX-G9CT-1a pGEX-4T-2 Bacteria expression vector of human galectin-9 (C-terminal CRD with a part of the linker peptide, G9CT), GST fusion protein
RDB08425 pGEX-G9NCRD-1a pGEX-4T-2 Bacteria expression vector of human galectin-9 (N-terminal CRD, G9NCRD), GST fusion protein
RDB08426 pGEX-G9CCRD-1a pGEX-4T-2 Bacteria expression vector of human galectin-9 (C-terminal CRD, G9CCRD), GST fusion protein
RDB08442 pGEX-mG9(M)-2a pGEX-4T-2 Bacteria expression vector of mouse galectin-9 (the predominant isoform of mG9 in most cell and tissue types (mG9(M)/mG9M), GST fusion protein
RDB08443 pET-mG9(M)-1a pET-11a Bacteria expression vector of mouse galectin-9 (the predominant isoform of mG9 in most cell and tissue types (mG9(M)/mG9M), tag-free protein
RDB08444 pET-mG9(L)-3a pET-11a Bacteria expression vector of mouse galectin-9 with a long linker peptide (mG9(L)/mG9L), tag-free protein
RDB08457 pET-mG9Null-5a pET-11a Bacteria expression vector of mouse galectin-9 with a truncated linker peptide (mG9Null), tag-free protein
RDB08458 pET-mG9NullR64H-1a pET-11a Bacteria expression vector of mouse galectin-9 with a truncated linker peptide (mG9Null) with an R64H mutation, tag-free protein
RDB08459 pET-mG9NullR211H-2a pET-11a Bacteria expression vector of mouse galectin-9 with a truncated linker peptide (mG9Null) with an R211H mutation, tag-free protein
RDB08447 pTrc-RaG9(M)-2a pTrc-HisB Bacteria expression vector of rat galectin-9 (the predominant isoform of RaG9 in most cell and tissue types (RaG9(M)/RaG9M), (His) 6 -containing-tag protein

Galectin-10

Catalog no. Name of resource Vector Running title
RDB08427 pGEX-G10-8a pGEX-4T-2 Bacteria expression vector of human galectin-10, GST fusion protein
RDB04224 pTrc-G10-4a pTrc-HisB Bacteria expression vector of human galectin-10, (His) 6 -containing-tag protein

Galectin-12

Catalog no. Name of resource Vector Running title
RDB08428 pGEX-G12-1a pGEX-4T-2 Bacteria expression vector of human galectin-12, GST fusion protein
RDB04225 pGEX-LNG12-3 pGEX-4T-2 Bacteria expression vector of human galectin-12 with an N-terminally extended NCRD (LNG12), GST fusion protein
RDB08429 pGEX-G12NT-1b pGEX-4T-2 Bacteria expression vector of human galectin-12 (N-terminal CRD with a part of the linker peptide, G12NT), GST fusion protein
RDB08430 pGEX-G12CT-1b pGEX-4T-2 Bacteria expression vector of human galectin-12 (C-terminal CRD with a part of the linker peptide, G12CT), GST fusion protein

Galectin-13

Catalog no. Name of resource Vector Running title
RDB08431 pGEX-G13-1a pGEX-4T-2 Bacteria expression vector of human galectin-13, GST fusion protein

Galectin-14/PPL13

Catalog no. Name of resource Vector Running title
RDB08432 pGEX-PPL13-1a pGEX-4T-2 Bacteria expression vector of human galectin-14/PPL13, GST fusion protein

Lectin

Link to KEGG BRITE – Lectins
List of gene resources

Reference


(GSB0048e 2020.10.03 T.M.)

2021.03.21



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