Nakano’s yeast clone collection

NAKANO AKIHIKO clone collection

Study in membrane trafficking of budding yeast, S. cereviciae.

Publication history

PMID TITLE Link
18343875 Takeda Y, Nakano A. (2008) In vitro formation of a novel type of membrane vesicles containing Dpm1p: putative transport vesicles for lipid droplets in budding yeast. J Biochem. 143, pp803-811. CLICK to check clones!
17973654 Higashio H1, Sato K, Nakano A. (2008) Smy2p participates in COPII vesicle formation through the interaction with Sec23p/Sec24p subcomplex. Traffic. 9, pp79-93. CLICK to check clones!
16735437 Yahara N, Sato K, Nakano A. (2006) The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus. J Cell Sci. 15, pp2604-2612. CLICK to check clones!
16699523 Matsuura-Tokita K, Takeuchi M, Ichihara A, Mikuriya K, Nakano A. (2006) Live imaging of yeast Golgi cisternal maturation. Nature 441, pp1007-1010 CLICK to check clones!
15665868 Sato K, Nakano A. (2005) Dissection of COPII subunit-cargo assembly and disassembly kinetics during Sar1p-GTP hydrolysis. Nat Struct Mol Biol. 12, pp167-174. CLICK to check clones!
14699055 Sato M, Sato K, Nakano A. (2004) Endoplasmic reticulum quality control of unassembled iron transporter depends on Rer1p-mediated retrieval from the golgi. Mol Biol Cell. 15, pp1417-1424. CLICK to check clones!
14627716 Sato K, Nakano A.(2004) Reconstitution of coat protein complex II (COPII) vesicle formation from cargo-reconstituted proteoliposomes reveals the potential role of GTP hydrolysis by Sar1p in protein sorting. J Biol Chem. 279, pp1330-1335. CLICK to check clones!
12972550 Sato K, Sato M, Nakano A. (2003) Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol Biol Cell. 14, pp3605-3616. CLICK to check clones!
12857885 Sato K, Nakano A. (2003) Oligomerization of a cargo receptor directs protein sorting into COPII-coated transport vesicles. Mol Biol Cell. 14, pp3055-3063. CLICK to check clones!
12810702 Umebayashi K, Nakano A. (2003) Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J Cell Biol. 161, pp1117-1131 CLICK to check clones!
12134087 Sato K, Nakano A. (2002) Emp47p and its close homolog Emp46p have a tyrosine-containing endoplasmic reticulum exit signal and function in glycoprotein secretion in Saccharomyces cerevisiae. Mol Biol Cell. 13, pp2518-2532. CLICK to check clones!
11442630 Sato M, Fujisaki S, Sato K, Nishimura Y, Nakano A. (2001) Yeast Saccharomyces cerevisiae has two cis-prenyltransferases with different properties and localizations. Implication for their distinct physiological roles in dolichol synthesis. Genes Cells. 6, pp495-506. CLICK to check clones!
11238450 Sato K, Sato M, Nakano A. (2001) Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamically localized to the Golgi apparatus by coatomer. J Cell Biol. 152, pp935-944 CLICK to check clones!
11168590 Saito-Nakano Y, Nakano A. (2000) Sed4p functions as a positive regulator of Sar1p probably through inhibition of the GTPase activation by Sec23p. Genes Cells. 5, pp1039-1048. CLICK to check clones!
11160834 Yahara N, Ueda T, Sato K, Nakano A. (2001) Multiple roles of Arf1 GTPase in the yeast exocytic and endocytic pathways. Mol Biol Cell. 12, pp221-238. CLICK to check clones!
11044978 Hopkins BD, Sato K, Nakano A, Graham TR. (2000) Introduction of Kex2 cleavage sites in fusion proteins for monitoring localization and transport in yeast secretory pathway. Methods Enzymol. 327, pp107-118. CLICK to check clones!
10791890 Matsuda N, Ueda T, Sasaki Y, Nakano A. (2000) Overexpression of PRA2, a Rab/Ypt-family small GTPase from Pea Pisum sativum, aggravates the growth defect of yeast ypt mutants. Cell Struct Funct. 25, pp11-20. CLICK to check clones!
10788782 Homma K, Yoshida Y, Nakano A. (2000) Evidence for recycling of cytochrome P450 sterol 14-demethylase from the cis-Golgi compartment to the endoplasmic reticulum (ER) upon saturation of the ER-retention mechanism. J Biochem. 127, pp747-754. CLICK to check clones!
9920934 Murakami A, Kimura K, Nakano A. (1999) The inactive form of a yeast casein kinase I suppresses the secretory defect of the sec12 mutant. Implication of negative regulation by the Hrr25 kinase in the vesicle budding from the endoplasmic reticulum. J Biol Chem. 274, pp3804-3810. CLICK to check clones!
9880808 Saito Y, Yamanushi T, Oka T, Nakano A. (1999) Identification of SEC12, SED4, truncated SEC16, and EKS1/HRD3 as multicopy suppressors of ts mutants of Sar1 GTPase. J Biochem. 125, pp130-137. CLICK to check clones!
9858571 Sato M, Sato K, Nishikawa S, Hirata A, Kato J, Nakano A. (1999) The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in dolichol synthesis. Mol Cell Biol. 19, pp471-483. CLICK to check clones!
9756629 Saito Y, Kimura K, Oka T, Nakano A. (1998) Activities of mutant Sar1 proteins in guanine nucleotide binding, GTP hydrolysis, and cell-free transport from the endoplasmic reticulum to the Golgi apparatus. J Biochem. 124, pp816-823. CLICK to check clones!
8889833 Yamanushi T, Hirata A, Oka T, Nakano A. (1996) Characterization of yeast sar1 temperature-sensitive mutants, which are defective in protein transport from the endoplasmic reticulum. J Biochem. 120, pp452-458. CLICK to check clones!
8707815 Sato M, Sato K, Nakano A. (1996) Endoplasmic reticulum localization of Sec12p is achieved by two mechanisms: Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain. J Cell Biol. 134,pp279-93 CLICK to check clones!
8589449 Sato K, Nishikawa S, Nakano A. (1995) Membrane protein retrieval from the Golgi apparatus to the endoplasmic reticulum (ER): characterization of the RER1 gene product as a component involved in ER localization of Sec12p. Mol Biol Cell. 6, pp1459-1477. CLICK to check clones!
8367481 Nishikawa S, Nakano A. (1993) Identification of a gene required for membrane protein retention in the early secretory pathway. Proc Natl Acad Sci U S A. 90, pp8179-8183. CLICK to check clones!
7865879 Nishikawa S, Hirata A, Nakano A. (1994) Inhibition of endoplasmic reticulum (ER)-to-Golgi transport induces relocalization of binding protein (BiP) within the ER to form the BiP bodies. Mol Biol Cell. 5, pp1129-1143. CLICK to check clones!
7822237 Nakano A, Otsuka H, Yamagishi M, Yamamoto E, Kimura K, Nishikawa S, Oka T. (1994) Mutational analysis of the Sar1 protein, a small GTPase which is essential for vesicular transport from the endoplasmic reticulum. J Biochem. 116, pp243-247. CLICK to check clones!
3047151 Nakano A, Brada D, Schekman R. (1988) A membrane glycoprotein, Sec12p, required for protein transport from the endoplasmic reticulum to the Golgi apparatus in yeast. J Cell Biol. 107, pp851-863. CLICK to check clones!
2512296 Nakano A, Muramatsu M. (1989) A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus. J Cell Biol. 109, pp2677-2691. CLICK to check clones!
1907491 Nishikawa S, Nakano A. (1991) The GTP-binding Sar1 protein is localized to the early compartment of the yeast secretory pathway. Biochim. Biophys. Acta. 1093, pp135-143 CLICK to check clones!

Clone Table

In vitro formation of a novel type of membrane vesicles containing Dpm1p: putative transport vesicles for lipid droplets in budding yeast. (2008) J Biochem. 143, pp803-811.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08715 pYTY102 CEN URA3 Ap PTDH3-DPM1-GFP-TCMK1

Smy2p participates in COPII vesicle formation through the interaction with Sec23p/Sec24p subcomplex. (2008) Traffic 9, pp79-93.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08718 pYPL1 URA3 Ap YPL105c SYH1: paralog of SMY2
RDB08719 pSMY1 URA3 Ap SMY2
RDB08720 pSMY3 CEN URA3 Ap SMY2
RDB08721 pSMY2 URA3 Ap SMY2-3HA
RDB08722 pSMY4 CEN URA3 Ap SMY2-3HA
RDB08723 pSMY5 CEN URA3 Ap smy2(mutant A)-3HA Smy2(W279A Y284A)
RDB08724 pSMY6 CEN URA3 Ap smy2(mutant B)-3HA Smy2Δ(269-285)
RDB08725 pSMY7 CEN URA3 Ap smy2(mutant C)-3HA Smy2Δ(420-490)
RDB08726 pSMY8 CEN URA3 Ap smy2(mutant D)-3HA Smy2Δ(337-790)
RDB08727 pSMY9 CEN URA3 Ap smy2(mutant E)-3HA Smy2Δ(337-676)
RDB08728 pSMY10 CEN URA3 Ap smy2(mutant F)-3HA Smy2Δ(337-500)
RDB08729 pSMY11 CEN URA3 Ap smy2(mutant G)-3HA Smy2Δ(501-676)
RDB08730 pSMY12 CEN URA3 Ap smy2(mutant H)-3HA Smy2Δ(52-336)
RDB08731 pSMY13 CEN URA3 Ap smy2(mutant I)-3HA Smy2Δ(52-500)
RDB08732 pJG-SEC23 HIS3 Ap PGAL1-B42-HA-SEC23 Y2H system (AD)
RDB08733 pJG-SEC24 HIS3 Ap PGAL1-B42-HA-SEC24 Y2H system (AD)
RDB08734 pEG-SEC24 TRP1 Ap PADH1-LexA-SEC24 Y2H system (BD)
RDB08735 pEG-SMY2 TRP1 Ap PADH1-LexA-SMY2 Y2H system (BD)

The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus. (2006) J Cell Sci. 15, pp2604-2612.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08549 pNY24-GLO3 TRP1 Ap GLO3
RDB08550 pNY24-3HA-GLO3 TRP1 Ap 3HA-GLO3
RDB08551 pNY24-GCS1 TRP1 Ap GCS1
RDB08552 pNY24-GCS1-3HA TRP1 Ap GCS1-3HA
RDB08553 pNY24-GLO3-A-GCS1 TRP1 Ap PGLO3-glo3-gcs1 Glo3(N-64)-Gcs1(57-C)
RDB08554 pNY24-GLO3-N-GCS1 TRP1 Ap PGLO3-glo3-gcs1 Glo3(N-122)-Gcs1(117-C)
RDB08555 pNY24-GCS1-A-GLO3 TRP1 Ap PGCS1-gcs1-glo3 Gcs1(N-60)-Glo3 (62-C)
RDB08556 pNY24-GCS1-N-GLO3 TRP1 Ap PGCS1-gcs1-glo3 Gcs1(N-111)-Glo3(119-C)
RDB08557 pNY24-AGE1 TRP1 Ap AGE1
RDB08558 pNY24-AGE2 TRP1 Ap AGE2

Live imaging of yeast Golgi cisternal maturation. (2006) Nature 441, pp1007-1010.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08658 pRS316-GFP-SED5-SB CEN URA3 Ap PTDH3-EGFP-SED5-TCMK1
RDB08660 pRS316-GFP-GOS1 CEN URA3 Ap PTDH3-EGFP-GOS1-TCMK1
RDB08662 pRS316-SEC7-GFP CEN URA3 Ap PADH1-SEC7-EGFP-TCMK1

Dissection of COPII subunit-cargo assembly and disassembly kinetics during Sar1p-GTP hydrolysis. (2005) Nat Struct Mol Biol. 12, pp167-174.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08689 pKSY193 URA3 Ap His6-YFP-SEC24
RDB08690 pKSY197 CEN URA3 Ap 2xHA-CFP-BET1
RDB08691 pKSY198 CEN URA3 Ap PTDH3-2xHA-CFP-Strep-SEC22-TCMK1
RDB08692 pET21a-SED5 bacterial Ap Sed5p (1-316)-His6 expression vector (T7 promoter, DE3 required)
RDB08708 pKSE165 bacterial Ap Strep2-CFP-Bet1 expression vector (tet promoter)
RDB08709 pKSE168 bacterial Ap Strep-CFP-Strep-Sec22 expression vector (tet promoter)
RDB08710 pKSE174 bacterial Ap Strep2-CFP-bet1(L54A E55A) expression vector (tet promoter)
RDB08711 pKSE176 bacterial Ap GST-Sec12(1-373) expression vector (tac promoer), Sec12Δluminal-domain
RDB08712 pKSE188 bacterial Cm His6-Bos1(1-222) expression vector (lac promoter)
RDB08713 pKSE203 bacterial Ap MBP-CFP-Ufe1 expression vector (tac promoter)

Endoplasmic reticulum quality control of unassembled iron transporter depends on Rer1p-mediated retrieval from the golgi. (2004) Mol Biol Cell. 15, pp1417-1424.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08805 pFET3-42 Int. Ap FET3-3xHA::URA3
RDB08806 pFET3-43 Int. Ap FET3-GFP::URA3
RDB08807 pFET3-52 Int. Ap FET3-3xHA::URA3::fet3(Δ5′ region) Cut BamHI, then trafo
RDB08808 pFET3-53 Int. Ap FET3-GFP::URA3::fet3(Δ5′ region) Cut BamHI, then trafo
RDB08809 pFET3-802NA Int. Ap FET3(NheI-TMD-AflII)-3HA::URA3 As RDB8805 except NheI & AflII at 560th & 584th codons, respectively
RDB08810 Int. Ap fet3[(TMD)560-584(24xL)]-3HA::URA3 Cut NspV, then trafo
RDB08811 pFET3-803NA Int. Ap FET3(NheI-TMD-AflII)-GFP::URA3 As RDB8806 except NheI & AflII at 560th & 584th codons, respectively.
RDB08812 pFET3-803L24 Int. Ap fet3[(TMD)560-584(24xL)]-GFP::URA3 Cut NspV, then trafo
RDB08813 pFET3-42B-T565L Int. Ap fet3(T565L)-3xHA ::URA3 Cut NspV, then trafo
RDB08814 pFET3-42B-S567L Int. Ap fet3(S567L)-3xHA ::URA3 Cut NspV, then trafo
RDB08815 pFET3-42B-T577L Int. Ap fet3(T577L)-3xHA ::URA3 Cut NspV, then trafo
RDB08816 pFET3-42B-Y581L Int. Ap fet3(Y581L)-3xHA ::URA3 Cut NspV, then trafo
RDB08817 pFET3-43B-T565L Int. Ap fet3(T565L)-3xHA ::URA3 Cut NspV, then trafo
RDB08818 pFET3-43B-S567L Int. Ap fet3(S567L)-3xHA ::URA3 Cut NspV, then trafo
RDB08819 pFET3-43B-T577L Int. Ap fet3(T577L)-GFP::URA3 Cut NspV, then trafo
RDB08820 pFET3-43B-Y581L Int. Ap fet3(Y581L)-GFP::URA3 Cut NspV, then trafo
RDB08821 pTU1-FET3-3HA CEN URA3 Ap PTDH3-FET3-3xHA
RDB08822 pTU1-FET3(L24)-3HA CEN URA3 Ap PTDH3-fet3[(TMD)560-584(24xL)]-3xHA::URA3
RDB08823 pTU1-FET3(S567L)-3HA CEN URA3 Ap PTDH3-fet3(S567L)-3xHA

Reconstitution of coat protein complex II (COPII) vesicle formation from cargo-reconstituted proteoliposomes reveals the potential role of GTP hydrolysis by Sar1p in protein sorting. (2004) J Biol Chem. 279, pp1330-1335.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08704 pKSE131 bacterial Ap Strep2-2xmyc-Emp47 expression vector (tet promoter)
RDB08705 pKSE132 bacterial Ap Strep2-3xHA-Emp46 expression vector (tet promoter)
RDB08706 pKSE133 bacterial Ap Strep2-myc-Emp47Δ(282-333) expression vector (tet promoter)
RDB08707 pKSE136 bacterial Ap MBP-Ufe1 expression vector (tac promoter)

Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. (2003) Mol Biol Cell. 14, pp3605-3616.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08580 p[SEC12-MFα1] (S12M) TRP1 Ap SEC12-MFα1
RDB08581 pRS316_MS71H CEN URA3 Ap PSEC71-MFα1-SEC71-HA
RDB08582 pYO326_MS71H URA3 Ap PSEC71-MFα1-SEC71-HA
RDB08583 pRS316_MS63F CEN URA3 Ap PSEC63-MFα1-SEC63-myc
RDB08584 pYO326_MS63F URA3 Ap PSEC63-MFα1-SEC63-myc
RDB08585 pSKY5wRER1-0 CEN TRP1 Ap PTDH3-EGFP-RER1-TCMK1
RDB08586 pSKY5wRER1Y68L CEN TRP1 Ap PTDH3-EGFP-rer1(Y68L)-TCMK1 Rer1(Y68L)
RDB08587 pSKY5wRER1N76L CEN TRP1 Ap PTDH3-EGFP-rer1(N76L)-TCMK1 Rer1(N76L)
RDB08588 pSKY5wRER1Q77L CEN TRP1 Ap PTDH3-EGFP-rer1(Q77L)-TCMK1 Rer1(Q77L)
RDB08589 pSKY5wRER1T83L CEN TRP1 Ap PTDH3-EGFP-rer1(T83L)-TCMK1 Rer1(T83L)
RDB08590 pSKY5wRER1T128L CEN TRP1 Ap PTDH3-EGFP-rer1(T128L)-TCMK1 Rer1(T128L)
RDB08591 pSKY5wRER1S131L CEN TRP1 Ap PTDH3-EGFP-rer1(S131L)-TCMK1 Rer1(S131L)
RDB08592 pSKY5wRER1S135L CEN TRP1 Ap PTDH3-EGFP-rer1(S135L)-TCMK1 Rer1(S135L)
RDB08593 pSKY5wRER1S138L CEN TRP1 Ap PTDH3-EGFP-rer1(S138L)-TCMK1 Rer1(S138L)
RDB08594 pSKY5wRER1Y152L CEN TRP1 Ap PTDH3-EGFP-rer1(Y152L)-TCMK1 Rer1(Y152L)
RDB08595 pSKY5wRER1T160L CEN TRP1 Ap PTDH3-EGFP-rer1(T160L)-TCMK1 Rer1(T160L)
RDB08598 pQR316-SEC71-GFP CEN URA3 Ap SEC71-GFP
RDB08599 pQR326-SEC71-GFP URA3 Ap SEC71-GFP
RDB08600 pRS306-SEC71TMD-GFP/LS Int. Ap sec71T(LS)-GFP::URA3 Sec71 (trans-membrane domain, L40S)
RDB08601 pRS306-SEC71TMD-GFP/SL Int. Ap sec71T(SL)-GFP::URA3 Sec71 (trans-membrane domain, S43L)
RDB08602 pRS306-SEC71TMD-GFP/YL Int. Ap sec71T(YL)-GFP::URA3 Sec71 (trans-membrane domain, Y36L)
RDB08603 pRS306-SEC71TMD-GFP/1L Int. Ap sec71T(1L)-GFP::URA3 Sec71 (trans-membrane domain, 1xL insert at 41)
RDB08604 pRS306-SEC71TMD-GFP/2L Int. Ap sec71T(2L)-GFP::URA3 Sec71 (trans-membrane domain, 2xL insert at 41)
RDB08605 pRS306-SEC71TMD-GFP/3L Int. Ap sec71T(3L)-GFP::URA3 Sec71 (trans-membrane domain, 3xL insert at 41)
RDB08606 pRS306-SEC71TMD-GFP/4L Int. Ap sec71T(4L)-GFP::URA3 Sec71 (trans-membrane domain, 4xL insert at 41)
RDB08607 pRS306-SEC71TMD-GFP/2LΔLV Int. Ap sec71T(2LΔLV)-GFP::URA3 Sec71 (trans-membrane domain, L44Δ V45Δ 2xL insert at 41)
RDB08608 pQR316-SEC71TMD-GFP/LS CEN URA3 Ap sec71T(LS)-GFP Sec71 (trans-membrane domain, L40S)
RDB08609 pQR316-SEC71TMD-GFP/SL CEN URA3 Ap sec71T(SL)-GFP Sec71 (trans-membrane domain, S43L)
RDB08610 pQR316-SEC71TMD-GFP/1L CEN URA3 Ap sec71T(1L)-GFP Sec71 (trans-membrane domain, 1xL insert at 41)
RDB08611 pQR316-SEC71TMD-GFP/2L CEN URA3 Ap sec71T(2L)-GFP Sec71 (trans-membrane domain, 2xL insert at 41)
RDB08612 pQR316-SEC71TMD-GFP/3L CEN URA3 Ap sec71T(3L)-GFP Sec71 (trans-membrane domain, 3xL insert at 41)
RDB08613 pQR316-SEC71TMD-GFP/4L CEN URA3 Ap sec71T(4L)-GFP Sec71 (trans-membrane domain, 4xL insert at 41)
RDB08614 pQR316-SEC71TMD-GFP/2LΔLV CEN URA3 Ap sec71T(2LΔLV)-GFP Sec71 (trans-membrane domain, L44Δ V45Δ 2xL insert at 41)
RDB08628 pRS316-MWWWK CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1 Wbp1 (C terminal 154 aa)
RDB08629 pRS316-MWWWS CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1(SSTN) Wbp1 [C terminal 154aa, KKTN(C terminus)SSTN]
RDB08630 pRS316-M71WWS CEN URA3 Ap PSEC63-MFα1-3xHA-sec71L-wbp1T-wbp1C(SSTN) Sec71(luminal)-Wbp1(TM)-Wbp1[cytosolic, KKTN(C terminus)SSTN]
RDB08631 pRS316-MW71WS CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1L-sec71T-wbp1C(SSTN) Wbp1(luminal)-Sec71(TM)-Wbp1[cytosolic, KKTN(C terminus)SSTN]
RDB08632 pRS316-MWW71 CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1L-wbp1T-sec71C Wbp1(luminal)-Wbp1(TM)-Sec71(cytosolic)
RDB08633 pRS316-M71W71 CEN URA3 Ap PSEC63-MFα1-3xHA-sec71L-wbp1T-sec71C Sec71(luminal)-Wbp1(TM)-Sec71(cytosolic)
RDB08634 pRS316-MW71WS/LS CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1L-sec71T(LS)-wbp1C(SSTN) Sec71 (trans-membrane domain, L40S)
RDB08635 pRS316-MW71WS/YL CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1L-sec71T(YL)-wbp1C(SSTN) Sec71 (trans-membrane domain, Y36L)
RDB08636 pRS316-MW71WS/SL CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1L-sec71T(SL)-wbp1C(SSTN) Sec71 (trans-membrane domain, S43L)
RDB08637 pRS316-MW71WS/ΔL CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1L-sec71T(ΔL)-wbp1C(SSTN) Sec71 (trans-membrane domain, L40Δ)
RDB08638 pRS316-MW71WS/1L CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1L-sec71T(1L)-wbp1C(SSTN) Sec71 (trans-membrane domain, 1xL insert at 41)
RDB08639 pRS316-MW71WS/2L CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1L-sec71T(2L)-wbp1C(SSTN) Sec71 (trans-membrane domain, 2xL insert at 41)
RDB08640 pRS316-MW71WS/3L CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1L-sec71T(3L)-wbp1C(SSTN) Sec71 (trans-membrane domain, 3xL insert at 41)
RDB08641 pRS316-MW71WS/4L CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1L-sec71T(4L)-wbp1C(SSTN) Sec71 (trans-membrane domain, 4xL insert at 41)
RDB08642 pRS316-MW71WS/2LΔLV CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1L-sec71T(2LΔLV)-wbp1C(SSTN) Sec71 (trans-membrane domain, L44Δ V45Δ 2xL insert at 41)
RDB08643 pRS316-MW71WS/YS CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1L-sec71T(YS)-wbp1C(SSTN) Sec71 (trans-membrane domain, Y36S)
RDB08644 pRS316-MW71WS/YQ CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1L-sec71T(YQ)-wbp1C(SSTN) Sec71 (trans-membrane domain, Y36Q)
RDB08645 pRS316-MW71WS/YA CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1L-sec71T(YA)-wbp1C(SSTN) Sec71 (trans-membrane domain, Y36A)
RDB08646 pRS316-MW71WS/SY CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1L-sec71T(SY)-wbp1C(SSTN) Sec71 (trans-membrane domain, S43Y)
RDB08647 pRS316-MW71WS/SQ CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1L-sec71T(SQ)-wbp1C(SSTN) Sec71 (trans-membrane domain, S43Q)
RDB08648 pRS316-MW71WS/SA CEN URA3 Ap PSEC63-MFα1-3xHA-wbp1L-sec71T(SA)-wbp1C(SSTN) Sec71 (trans-membrane domain, S43A)
RDB08651 pRS316-GFP-SEC12 CEN URA3 Ap GFP-SEC12
RDB08652 pRS306-SEC71TMD-GFP Int. Ap URA3::sec71T-GFP::URA3 Sec71(trans-membrane domain)
RDB08653 pQR316-SEC71TMD-GFP CEN URA3 Ap sec71T-GFP Sec71(trans-membrane domain)
RDB08657 pSKY54-SEC12 CEN URA3 Ap GFP-SEC12

Oligomerization of a cargo receptor directs protein sorting into COPII-coated transport vesicles. (2003) Mol Biol Cell. 14, pp3055-3063.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08683 pKSY155 CEN TRP1 Ap GFP-myc-emp47(Δ383) Emp47Δ(383-C terminus)
RDB08684 pKSY160 CEN TRP1 Ap GFP-myc-emp47(Δ333) Emp47Δ(333-C terminus)
RDB08685 pKSY166 CEN TRP1 Ap GFP-myc-emp47(Δ281) Emp47Δ(281-C terminus)
RDB08686 pKSY169 TRP1 Ap myc-EMP47
RDB08687 pKSY177 CEN TRP1 Ap myc-emp47(Δ281-333) Emp47Δ(281-333)
RDB08688 pKSY187 CEN URA3 Ap HA-emp46(Δ279-321) Emp46Δ(279-321)

Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. (2003) J Cell Biol. 161, pp1117-1131.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08559 pKU1 bacterial Ap TAT2 cloning vector
RDB08560 pKU41 Int. Ap tat2Δ::ADE2 Cut EcoRI & SphI, then trafo
RDB08561 pKU46 CEN URA3 Ap TAT2-3xHA
RDB08562 pKU51 Int. Ap TAT2-3xHA::URA3 Cut PmaCI, then trafo
RDB08563 pKU60 Int. Ap pep4Δ::HIS3 Cut EcoRI & SalI, then trafo
RDB08564 pKU62 Int. Ap vps1Δ::HIS3 Cut SphI, then trafo
RDB08565 pKU65 Int. Ap vps27Δ::HIS3 Cut EcoRI & SalI, then trafo
RDB08566 pKU68 URA3 Ap TAT2
RDB08567 pKU76 CEN URA3 Ap TAT2-GFP
RDB08568 pKU82 Int. Ap tat2Δ::hisG-URA3-hisG Cut EcoRI & SphI, then trafo
RDB08569 pKU84 CEN URA3 Ap GFP-PEP12
RDB08570 pKU105 CEN URA3 Ap PCUP1-ub-TCYC1 ub: synthetic yeast ubiquitin
RDB08571 pKU106 CEN URA3 Ap PCUP1-myc-ub-TCYC1 ub: synthetic yeast ubiquitin
RDB08572 pKU108 Int. Ap doa4Δ::HIS3 Cut SacI & SphI, then trafo
RDB08573 pKU121 Int. Ap bul1Δ::HIS3 Cut EcoRI & SalI, then trafo
RDB08574 pKU131 Int. Ap erg13Δ::HIS3 Cut EcoRI & SphI, then trafo
RDB08575 pKU158 CEN URA3 Ap tat23KR Tat2(K10R K17R K20R)

Emp47p and its close homolog Emp46p have a tyrosine-containing endoplasmic reticulum exit signal and function in glycoprotein secretion in Saccharomyces cerevisiae. (2002) Mol Biol Cell. 13, pp2518-2532.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08665 pKSY103 CEN URA3 Ap EMP46
RDB08666 pKSY104 URA3 Ap EMP46
RDB08667 pKSY105 CEN TRP1 Ap 2xmyc-EMP47
RDB08668 pKSY113 CEN URA3 Ap 3xHA-EMP46
RDB08669 pKSY114 URA3 Ap 3xHA-EMP46
RDB08670 pKSY115 CEN URA3 Ap 3xHA-emp46(ΔKVKLL) Emp46(ΔC-terminal KVKLL)
RDB08671 pKSY116 CEN URA3 Ap 3xHA-emp46(RVRLL) Emp46[KVKLL(C-terminus)RVRLL]
RDB08672 pKSY117 CEN URA3 Ap 3xHA-emp46(AYMF) Emp46[VKLL(C-terminus)AYMF]
RDB08673 pKSY118 CEN URA3 Ap 3xHA-emp46(YYMA) Emp46[VKLL(C-terminus)YYMA]
RDB08674 pKSY121 CEN URA3 Ap 3xHA-emp46(AYMA) Emp46[VKLL(C-terminus)AYMA]
RDB08675 pKSY122 CEN URA3 Ap 3xHA-emp46(KVKAA) Emp46[KVKLL(C-terminus)KVKAA]
RDB08676 pKSY123 CEN URA3 Ap 3xHA-emp46(SVSLL) Emp46[KVKLL(C-terminus)SVSLL]
RDB08677 pKSY126 CEN URA3 Ap GFP-3xHA-EMP46
RDB08678 pKSY131 CEN URA3 Ap GFP-3xHA-emp46(SVSLL) Emp46[KVKLL(C-terminus)SVSLL]
RDB08679 pKSY132 CEN URA3 Ap GFP-3xHA-emp46(AYFM) Emp46[VKLL(C-terminus)AYFM]
RDB08680 pKSY133 CEN URA3 Ap GFP-3xHA-emp46(YYMA) Emp46[VKLL(C-terminus)YYMA]
RDB08681 pKSY134 CEN URA3 Ap GFP-3xHA-emp46(AYMA) Emp46[VKLL(C-terminus)AYMA]
RDB08682 pKSY135 CEN URA3 Ap GFP-3xHA-emp46(KVKAA) Emp46[KVKLL(C-terminus)KVKAA]
RDB08693 pKSE106 bacterial Ap Emp46p (1-412) expression vector (T7 promoter, DE3 required)
RDB08694 pKSE107 Int. Ap emp46Δ::HIS3
RDB08695 pKSE108 Int. Ap emp47Δ::LEU2
RDB08696 pKSE112 bacterial Ap GST-Emp46Ct expression vector (T7 promoter, DE3 required), Emp46(C-terminal 17AA)
RDB08697 pKSE113 bacterial Ap GST-Emp46Ct(AYMF) expression vector (T7 promoter, DE3 required), Emp46[C-terminal 17AA, VKLL(C-terminus)AYMF]
RDB08698 pKSE114 bacterial Ap GST-Emp46Ct(YYMA) expression vector (T7 promoter, DE3 required), Emp46[C-terminal 17AA, VKLL(C-terminus)YYMA]
RDB08699 pKSE115 bacterial Ap GST-Emp46Ct(AYMA) expression vector (T7 promoter, DE3 required), Emp46[C-terminal 17AA, VKLL(C-terminus)AYMA]
RDB08700 pKSE116 bacterial Ap GST-Emp46Ct(ΔKVKLL) expression vector (T7 promoter, DE3 required), Emp46(C-terminal 17AA, ΔC-terminal KVKLL)
RDB08701 pKSE117 bacterial Ap GST-Emp46Ct(KVKAA) expression vector (T7 promoter, DE3 required), Emp46[C-terminal 17AA, KVKLL(C-terminus)KVKAA]
RDB08702 pKSE121 bacterial Ap GST-Emp7Ct expression vector (T7 promoter, DE3 required), Emp46(C-terminal 17AA)
RDB08703 pKSE122 bacterial Ap GST-Emp47Ct(STSLL) expression vector (T7 promoter, DE3 required), Emp46[C-terminal 17AA, KTKLL(C-terminus)STSLL]

Yeast Saccharomyces cerevisiae has two cis-prenyltransferases with different properties and localizations. Implication for their distinct physiological roles in dolichol synthesis. (2001) Genes Cells. 6, pp495-506.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08824 pR9-0 bacterial Ap RER2 cloning vector
RDB08825 pΔrer2::HIS3 Int. Ap rer2Δ::HIS3 Cut ScaI & EcoRV, then trafo
RDB08826 pΔsrt1::ADE2 Int. Ap srt1Δ::ADE2 Cut XhoI & EcoRI, then trafo
RDB08827 pΔsrt1::LEU2 Int. Ap srt1Δ::LEU2 Cut XhoI & PvuII, then trafo
RDB08828 pSRT-2 CEN URA3 Ap SRT1
RDB08829 pSRT-3 URA3 Ap SRT1
RDB08830 pSRTHA-2 CEN URA3 Ap SRT1-3xHA
RDB08831 pSRTHA-3 URA3 Ap SRT1-3xHA
RDB08832 pSKY5-RER2 CEN URA3 Ap PTDH3-EGFP-RER2-TCMK1
RDB08833 pSKY5-SRT1 CEN URA3 Ap PTDH3-EGFP-SRT1-TCMK1
RDB08834 pEG6-4 CEN TRP1 Ap ERG6-3xmyc

Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamically localized to the Golgi apparatus by coatomer. (2001) J Cell Biol. 152, pp935-944.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08615 pSKY5-RER1-0 CEN URA3 Ap PTDH3-EGFP-RER1-TCMK1
RDB08616 pSKY5-RER1-5 CEN URA3 Ap PTDH3-EGFP-rer1(-5)-TCMK1 Rer1(ΔC-terminal 5aa)
RDB08617 pSKY5-RER1-10 CEN URA3 Ap PTDH3-EGFP-rer1(-10)-TCMK1 Rer1(ΔC-terminal 10aa)
RDB08618 pSKY5-RER1-15 CEN URA3 Ap PTDH3-EGFP-rer1(-15)-TCMK1 Rer1(ΔC-terminal 15aa)
RDB08619 pSKY5-RER1-20 CEN URA3 Ap PTDH3-EGFP-rer1(-20)-TCMK1 Rer1(ΔC-terminal 20aa)
RDB08620 pSKY5-RER1-25 CEN URA3 Ap PTDH3-EGFP-rer1(-25)-TCMK1 Rer1(ΔC-terminal 25aa)
RDB08621 pSKY5-RER1 G179A CEN URA3 Ap PTDH3-EGFP-rer1(G179A)-TCMK1 Rer1(G179A)
RDB08622 pSKY5-RER1-K180S CEN URA3 Ap PTDH3-EGFP-rer1(K180S)-TCMK1 Rer1(K180S)
RDB08623 pSKY5-RER1-K181S CEN URA3 Ap PTDH3-EGFP-rer1(K181S)-TCMK1 Rer1(K181S)
RDB08624 pSKY5-RER1-K182S CEN URA3 Ap PTDH3-EGFP-rer1(K182S)-TCMK1 Rer1(K182S)
RDB08625 pSKY5-RER1-Y183A CEN URA3 Ap PTDH3-EGFP-rer1(Y183A)-TCMK1 Rer1(Y183A)
RDB08626 pSKY5-RER1-Y173A CEN URA3 Ap PTDH3-EGFP-rer1(Y173A)-TCMK1 Rer1(Y173A)
RDB08627 pSKY5-RER1-KKSS CEN URA3 Ap PTDH3-EGFP-rer1(K180S K181S)-TCMK1 Rer1(K180S K181S)
RDB08655 pSKY5 CEN URA3 Ap PTDH3-EGFP-TCMK1
RDB08791 p316-HA-STE2 CEN URA3 Ap A-STE2-TCMK1
RDB08792 p316-HA-ste2-rer1 CEN URA3 Ap HA-ste2-rer1-TCMK1 Ste2(N-297)-Rer1(161-C)
RDB08793 pGST-Wbp1 bacterial Ap GST-Wbp1Ct (K421-N430) expression vector (tac prompoter)
RDB08794 pGST-Wbp1-SS bacterial Ap GST-Wbp1Ct [K421-N430 (K427S K428S)] expression vector (tac prompoter)
RDB08795 pGST-Rer1C28 bacterial Ap GST-Rer1Ct (M161-N188) expression vector (tac prompoter)
RDB08796 pGST-Rer1C28-KKSS bacterial Ap GST-Rer1Ct [M161-N188 (K180S K181S)] expression vector (tac prompoter)
RDB08797 pGST-Rer1C28-Y173A bacterial Ap GST-Rer1Ct [M161-N188 (Y173A)] expression vector (tac prompoter)
RDB08798 pGST-Rer1C28-Y183A bacterial Ap GST-Rer1Ct [M161-N188 (Y183A)] expression vector (tac prompoter)
RDB08799 Pgap-Dap2 URA3 Ap PTDH3-DAP2
RDB08800 Pgap-DSD URA3 Ap PTDH3-dap2C-sec12T-dap2L Dap2(cytosolic)-Sec12(TM)-Dap2(luminal)
RDB08801 Pgap-DSD-NQ/L URA3 Ap PTDH3-dap2C-sec12T(N358L Q370L)-dap2L Dap2(cytosolic)-Sec12(TM, N358L Q370L)-Dap2(luminal)
RDB08802 Pgap-DSD-SY/L URA3 Ap PTDH3-dap2C-sec12T(S366L Y367L)-dap2L Dap2(cytosolic)-Sec12(TM, N366L Q367L)-Dap2(luminal)
RDB08803 Pgap-DSD-+2L URA3 Ap PTDH3-dap2C-sec12T(2xL ins 365)-dap2L Dap2(cytosolic)-Sec12(TM, 2xL insert at 365)-Dap2(luminal)
RDB08804 Pgap-DSD-L19 URA3 Ap PTDH3-dap2C-(19xLeu)-dap2L Dap2(cytosolic)-(19xLeu)-Dap2(luminal)

Sed4p functions as a positive regulator of Sar1p probably through inhibition of the GTPase activation by Sec23p. (2000) Genes Cells. 5, pp1039-1048.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08664 pYS28-E52 CEN TRP1 Ap SED4-3HA
RDB08766 pYS6-7 TRP1 Ap sar1(D32G)
RDB08767 pYS6 TRP1 Ap sar(E112K)
RDB08768 pYS25 URA3 Ap SED4
RDB08769 pRS316-SEC16 CEN URA3 Ap SEC16
RDB08770 pSKY2-2 CEN URA3 Ap PGAL1-Sec12 (1-354)-TCMK1

Multiple roles of Arf1 GTPase in the yeast exocytic and endocytic pathways. (2001) Mol Biol Cell. 12, pp221-238.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08576 YIpARF1 Int. Ap ADE2::ARF1::ADE2 Cut HpaI, then trafo
RDB08577 p(arf1Δ::HIS3) Int. Ap arf1Δ::HIS3 Cut EcoRI & SphI, then trafo
RDB08578 p(arf2Δ::HIS3) Int. Ap arf2Δ::HIS3 Cut PvuII, then trafo
RDB08579 pNY14-A1S CEN TRP1 Ap ARF1
RDB08736 pNY16-A1 CEN URA3 Ap EcoRI-ARF1
RDB08737 pNY605 CEN TRP1 Ap arf1-11 Arf1(K38I E132D L173S)
RDB08738 pNY607NB CEN TRP1 Ap arf1-12 Arf1(K38I)
RDB08739 pNY801 CEN TRP1 Ap arf1-13 Arf1(L173S)
RDB08740 pNY1004 CEN TRP1 Ap arf1-14 Arf1(F51I)
RDB08741 pNY606NB CEN TRP1 Ap arf1-15 Arf1(L25P)
RDB08743 pNY902NB CEN TRP1 Ap arf1-17 Arf1(E41V)
RDB08744 pNY1005CB CEN TRP1 Ap arf1-18 Arf1(H80P)
RDB08745 p(605-ADE2) Int. Ap ADE2::arf1-11::ADE2 Arf1(K38I E132D L173S)
RDB08746 p(607NB-ADE2) Int. Ap ADE2::arf1-12::ADE2 Arf1(K38I)
RDB08747 p(801-ADE) Int. Ap ADE2::arf1-13::ADE2 Arf1(L173S)
RDB08748 p(606NB-ADE2) Int. Ap ADE2::arf1-14::ADE2 Arf1(F51I)
RDB08749 p(902-ADE2) Int. Ap ADE2::arf1-15::ADE2 Arf1(L25P)
RDB08750 p(902NB-ADE2) Int. Ap ADE2::arf1-17::ADE2 Arf1(E41V)
RDB08751 p(1005CB-ADE2) Int. Ap ADE2::arf1-18::ADE2 Arf1(H80P)
RDB08931 pNY902 CEN TRP1 Ap arf1-16 Arf1(E41V D129E)

Introduction of Kex2 cleavage sites in fusion proteins for monitoring localization and transport in yeast secretory pathway. (2000) Methods Enzymol. 327, pp107-118.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08864 pBAR1ΔH Int. Ap bar1Δ::HIS3 Cut SalI & XbaI, then trafo
RDB08865 palpha1ΔA1 Int. Ap mfα1Δ::ADE2 Cut EcoRI, then trafo
RDB08866 palpha2ΔT Int. Ap mfα2Δ::TRP1 Cut SalI & BglII, then trafo

<tdpra2(δc-terminal 3aa),=”” pisum=”” sativum=””>

Overexpression of PRA2, a Rab/Ypt-family small GTPase from Pea Pisum sativum, aggravates the growth defect of yeast ypt mutants. (2000) Cell Struct Funct. 25, pp11-20.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08752 pYO773 CEN URA3 Ap PGAL1-(mcs)-TCMK1 mcs: EcoRI BglII HindIII HpaI
RDB08753 pNMV3 CEN HIS3 Ap PGAL1-(mcs)-TCMK1 mcs: HpaI? EcoRI BglII
RDB87540 pNMV4 CEN TRP1 Ap PGAL1-(mcs)-TCMK1 mcs: HpaI EcoRI BglII in mcs
RDB08755 pGPR3 CEN URA3 Ap PGAL1-PRA2-TCMK1 Pra2, origin: Pisum sativum
RDB08756 pGPR3C CEN URA3 Ap PGAL1-PRA2?C-TCMK1
RDB08757 pGPR334 CEN URA3 Ap PGAL1-PRA2(T34N)-TCMK1 Pra2(T34N), origin: Pisum sativum
RDB08758 pGPR352 CEN URA3 Ap PGAL1-PRA2(T52A)-TCMK1 Pra2(T52A), origin: Pisum sativum
RDB08759 pGPR379 CEN URA3 Ap PGAL1-PRA2(Q79L)-TCMK1 Pra2(Q79L), origin: Pisum sativum
RDB08760 pGPR3133 CEN URA3 Ap PGAL1-PRA2(N133I)-TCMK1 Pra2(N133I), origin: Pisum sativum
RDB08761 pGGR3D CEN URA3 Ap PGAL1-GDI1-TCMK1 PGAL1-PRA2-TCMK1 Pra2, origin: Pisum sativum
RDB08762 pGPR4 CEN HIS3 Ap PGAL1-PRA2-TCMK1 Pra2, origin: Pisum sativum
RDB08763 pGPR5 CEN TRP1 Ap PGAL1-PRA2-TCMK1 Pra2, origin: Pisum sativum
RDB08764 pNMY191 CEN URA3 Ap PGAL1-GDI1-TCMK1
RDB08765 pDSS41 CEN URA3 Ap PGAL1-DSS4-TCMK1

Evidence for recycling of cytochrome P450 sterol 14-demethylase from the cis-Golgi compartment to the endoplasmic reticulum (ER) upon saturation of the ER-retention mechanism. (2000) J Biochem. 127, pp747-754.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08788 pYO326-chimeraN URA3 Ap Ngs-ERG11-3xHA Ngs:? potentiated N-linked glycosylation site [Erg11(L283MKNSTYKDGVKMTDQEIANLLIGVL312)]
RDB08789 pRS316-chimeraN CEN URA3 Ap Ngs-ERG11-3xHA Ngs:? potentiated N-linked glycosylation site [Erg11(L283MKNSTYKDGVKMTDQEIANLLIGVL312)]
RDB08790 pYO326-ERG11/CYP51 URA3 Ap ERG11

The inactive form of a yeast casein kinase I suppresses the secretory defect of the sec12 mutant. Implication of negative regulation by the Hrr25 kinase in the vesicle budding from the endoplasmic reticulum. (1999) J Biol Chem. 274, pp3804-3810.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08778 pAM5-2 TRP1 Ap 3xHA-HRR25
RDB08779 pAM6-2 TRP1 Ap 3xHA-hrr25-2 as rst2-1, Hrr25(T176I)
RDB08780 pAM4-1 URA3 Ap PGAL1-3xHA-HRR25-TCYC1
RDB08781 pAM8-2 URA3 Ap PGAL1-3xHA-hrr25(K38A)-TCYC1
RDB08782 pAM2-326 URA3 Ap HRR25
RDB08783 pAM1-5 TRP1 Ap hrr25-2 as rst2-1, Hrr25(T176I)
RDB08784 pAM1-2 CEN TRP1 Ap hrr25-2 as rst2-1, Hrr25(T176I)
RDB08785 pAM8-1 (K38A) TRP1 Ap hrr25(K38A)
RDB08786 pAM13-2 CEN URA3 Ap sec12-4 Sec12(P73L)
RDB08787 pAM13-3 URA3 Ap sec12-4 Sec12(P73L)

Identification of SEC12, SED4, truncated SEC16, and EKS1/HRD3 as multicopy suppressors of ts mutants of Sar1 GTPase. (1999) J Biochem. 125, pp130-137.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08771 pYO12 LEU2 Ap SEC12
RDB08772 pYOSAR LEU2 Ap SAR1
RDB08773 pANY4-2 LEU2 Ap SED4
RDB08774 pYS3 URA3 Ap HRD3
RDB08775 pYO112KS-1 LEU2 Ap HRD3
RDB08776 pYS1-6 bacterial Ap HRD3 cloning vector
RDB08777 pYS2-2B Int. Ap hrd3Δ::TRP1 Cut KpnI & SacI, then trafo

The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in dolichol synthesis. (1999) Mol Cell Biol. 19, pp471-483.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08835 pY1 URA3 Ap RER2
RDB08836 p(rer2::LEU2) Int. Ap rer2Δ::LEU2 Cut HindIII, then trafo
RDB08837 p(srt1::TRP1) Int. Ap srt1Δ::TRP1 Cut XhoI & PvuII, then trafo
RDB08838 pR7 CEN URA3 Ap RER2
RDB08839 pR7HA CEN URA3 Ap 3xHA-RER2
RDB08840 pR7HA-4 URA3 Ap 3xHA-RER2
RDB08841 pR7HA-2 CEN TRP1 Ap 3xHA-RER2

Activities of mutant Sar1 proteins in guanine nucleotide binding, GTP hydrolysis, and cell-free transport from the endoplasmic reticulum to the Golgi apparatus. (1998) J Biochem. 124, pp816-823.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08876 pMYE3-1 bacterial Ap Sar1 expression vector (T7 promoter, DE3 required), cDNA derivative
RDB08877 pMYE3-2 bacterial Ap Sar1(K36M) expression vector (T7 promoter, DE3 required), cDNA derivative
RDB08878 pMYE3-3 bacterial Ap Sar1(D73V) expression vector (T7 promoter, DE3 required), cDNA derivative
RDB08879 pMYE3-4 bacterial Ap Sar1(H77L) expression vector (T7 promoter, DE3 required), cDNA derivative
RDB08880 pMYE3-5 bacterial Ap Sar1(N132I) expression vector (T7 promoter, DE3 required), cDNA derivative
RDB08881 pMYE3-6 bacterial Ap Sar1(C171S) expression vector (T7 promoter, DE3 required), cDNA derivative
RDB08882 pMYE3-7 bacterial Ap Sar1(D32G) expression vector (T7 promoter, DE3 required), cDNA derivative
RDB08883 pMYE3-8 bacterial Ap Sar1(T54A) expression vector (T7 promoter, DE3 required), cDNA derivative
RDB08884 pMYE3-9 bacterial Ap Sar1(E112K) expression vector (T7 promoter, DE3 required), cDNA derivative

Characterization of yeast sar1 temperature-sensitive mutants, which are defective in protein transport from the endoplasmic reticulum. (1996) J Biochem. 120, pp452-458.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08914 pTY3 CEN LEU2 Ap NcoI-SAR1 genomic derivative (intron at 10th codon)
RDB08915 pTY5 CEN LEU2 Ap PGAL1-SAR1 genomic derivative (intron at 10th codon)
RDB08917 pMYT3-7 CEN LEU2 Ap NcoI-sar1(D32G) cDNA derivative

Endoplasmic reticulum localization of Sec12p is achieved by two mechanisms: Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain. (1996) J Cell Biol. 134, pp279-93.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08842 pMS2-3 (pSSS-1) bacterial Ap SEC12(D470E)-MFα1 just for construction of RDB08844-RDB08846
RDB08843 pDDD-2 (pSD1-2, pMD2-6) URA3 Ap DAP2-MFα1
RDB08844 pDSS-2 (pSD4-2) URA3 Ap dap2L-sec12T-sec12C-MFα1 Dap2(luminal)-Sec12(TM)-Sec12(cytosolic)
RDB08845 pDDS-2 (pSD3-2) URA3 Ap dap2L-dap2T-sec12C-MFα1 Dap2(luminal)-Dap2(TM)-Sec12(cytosolic)
RDB08846 pSDS-2 (pSD2-2) URA3 Ap sec12L-dap2T-sec12C-MFα1 Sec12(luminal)-Dap2(TM)-Sec12(cytosolic)
RDB08847 pSSD-2 (pSD7-2) URA3 Ap sec12L-sec12T-dap2C-MFα1 Sec12(luminal)-Sec12(TM)-Dap2(cytosolic)
RDB08848 pDSD-2 (pSD5-2) URA3 Ap dap2L-sec12T-dap2C-MFα1 Dap2(luminal)-Sec12(TM)-Dap2(cytosolic)
RDB08849 pSDD-2 (pSD6-2) URA3 Ap sec12L-dap2T-dap2C-MFα1 Sec12(luminal)-Dap2(TM)-Sec12(cytosolic)
RDB08850 pDSDm F-A URA3 Ap dap2L-sec12T(F355A F356A F359A)-dap2C-MFα1 Dap2(luminal)-Sec12(TM, F355A F356A F359A)-Dap2(cytosolic)
RDB08851 pDSDm SY-A URA3 Ap dap2L-sec12T(S366A Y367A S372A)-dap2C-MFα1 Dap2(luminal)-Sec12(TM, S366A Y367A S372A)-Dap2(cytosolic)
RDB08852 pDSDm N-A URA3 Ap dap2L-sec12T(N358A)-dap2C-MFα1 Dap2(luminal)-Sec12(TM, N358A)-Dap2(cytosolic)
RDB08853 pDSDm Q-A URA3 Ap dap2L-sec12T(Q370A)-dap2C-MFα1 Dap2(luminal)-Sec12(TM, Q370A)-Dap2(cytosolic)
RDB08854 pDSDm NQ-A URA3 Ap dap2L-sec12T(N358A Q370A)-dap2C-MFα1 Dap2(luminal)-Sec12(TM, N358A Q370A)-Dap2(cytosolic)
RDB08855 pDSDm A10 URA3 Ap dap2L-sec12T(F355A F356A T357A N358A F369A S366A Y367A Q370A F371A S372A)-dap2C-MFα1 Dap2(luminal)-Sec12(TM, F356A F357A T358A N359A F360A S366A Y367A Q370A F371A S372A)-Dap2(cytosolic)
RDB08856 pDSDm N-L URA3 Ap dap2L-sec12T(N358L)-dap2C-MFα1 Dap2(luminal)-Sec12(TM, N358L)-Dap2(cytosolic)
RDB08857 pDSDm Q-L URA3 Ap dap2L-sec12T(Q370L)-dap2C-MFα1 Dap2(luminal)-Sec12(TM, Q370L)-Dap2(cytosolic)
RDB08858 pDSDm NQ-L URA3 Ap dap2L-sec12T(N358L Q370L)-dap2C-MFα1 Dap2(luminal)-Sec12(TM, N358L Q370L)-Dap2(cytosolic)
RDB08859 pDSDm-L7 URA3 Ap dap2L-sec12T(T357L N358L S366L Y367L Q370L S372L)-dap2C-MFα1 Dap2(luminal)-Sec12(TM, T357L N358L S366L Y367L Q370L S372L)-Dap2(cytosolic)
RDB08860 p(LeuX19) URA3 Ap dap2L-(19xLeu)-dap2C-MFα1 Dap2(luminal)-(19xLeu)-Dap2(cytosolic)
RDB08861 pD4Dm-2 URA3 Ap dap2L-sed4T-dap2C-MFα1 Dap2(luminal)-Sed4(TM)-Dap2(cytosolic)
RDB08862 pD20Dm-2 URA3 Ap dap2L-sec20T-dap2C-MFα1 Dap2(luminal)-Sec20(TM)-Dap2(cytosolic)
RDB08863 p4DDm-2 URA3 Ap sed4L-dap2T-dap2C-MFα1 Sed4(luminal)-Dap2(TM)-Dap2(cytosolic)

Membrane protein retrieval from the Golgi apparatus to the endoplasmic reticulum (ER): characterization of the RER1 gene product as a component involved in ER localization of Sec12p. (1995) Mol Biol Cell. 6, pp1459-1477.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08596 pYO324/RER1-3HA TRP1 Ap RER1-3HA
RDB08597 pYO324/RER1-3HA Y152L TRP1 Ap rer1(Y152L)-3HA Rer1(Y152L)
RDB08649 pSK/rer1::LEU2 Int. Ap rer1Δ::LEU2
RDB08654 pRS314/RERI-3HA CEN TRP1 Ap RER1-3HA

Identification of a gene required for membrane protein retention in the early secretory pathway. (1993) Proc Natl Acad Sci U S A. 90, pp8179-8183.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08870 pSHF2-1 bacterial Ap SEC12 cloning vector
RDB08871 pSHY6-3 URA3 Ap SEC12
RDB08872 pSHF9-4 URA3 Ap SEC12-MFα1Δ(1-10) MFα1(signal peptide truncated)
RDB08873 pSHF9-4ΔSal URA3 Ap sec12(1-275)-MFα1Δ(1-10) MFα1(signal peptide truncated)
RDB08874 pSHF11-1 URA3 Ap SEC12(D470E)-SUC2Δ(1-21) Suc2(signal peptide truncated)

Inhibition of endoplasmic reticulum (ER)-to-Golgi transport induces relocalization of binding protein (BiP) within the ER to form the BiP bodies. (1994) Mol Biol Cell. 5, pp1129-1143.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08867 pSHY13-2 URA3 Ap PGAL1-SEC12
RDB08868 pSHY14-5 URA3 Ap PGAL1-SED4
RDB08869 pSHY15-1 TRP1 Ap PGAL1-SEC23-TCMK1

Mutational analysis of the Sar1 protein, a small GTPase which is essential for vesicular transport from the endoplasmic reticulum. (1994) J Biochem. 116, pp243-247.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08885 pMYY3-1 CEN TRP1 Ap SAR1 cDNA derivative
RDB08886 pMYY3-2 CEN TRP1 Ap sar1(K36M) cDNA derivative
RDB08887 pMYY3-3 CEN TRP1 Ap sar1(D73V) cDNA derivative
RDB08888 pMYY3-4 CEN TRP1 Ap sar1(H77L) cDNA derivative
RDB08889 pMYY3-5 CEN TRP1 Ap sar1(N132I) cDNA derivative
RDB08890 pMYY3-6 CEN TRP1 Ap sar1(C171S) cDNA derivative
RDB08891 pMYY3-7 CEN TRP1 Ap sar1(D32G) cDNA derivative
RDB08892 pMYY3-8 CEN TRP1 Ap sar1(T54A) cDNA derivative
RDB08893 pMYY3-9 CEN TRP1 Ap sar1(E112K) cDNA derivative
RDB08894 pMYY4-1 CEN TRP1 Ap PGAL1-SAR1 cDNA derivative
RDB08895 pMYY4-2 CEN TRP1 Ap PGAL1-sar1(K36M) cDNA derivative
RDB08896 pMYY4-3 CEN TRP1 Ap PGAL1-sar1(D73V) cDNA derivative
RDB08897 pMYY4-4 CEN TRP1 Ap PGAL1-sar1(H77L) cDNA derivative
RDB08898 pMYY4-5 CEN TRP1 Ap PGAL1-sar1(N132I) cDNA derivative
RDB08899 pMYY4-6 CEN TRP1 Ap PGAL1-sar1(C171S) cDNA derivative
RDB08900 pMYY4-7 CEN TRP1 Ap PGAL1-sar1(D32G) cDNA derivative
RDB08901 pMYY4-8 CEN TRP1 Ap PGAL1-sar1(T54A) cDNA derivative
RDB08902 pMYY4-9 CEN TRP1 Ap PGAL1-sar1(E112K) cDNA derivative

A membrane glycoprotein, Sec12p, required for protein transport from the endoplasmic reticulum to the Golgi apparatus in yeast. (1988) J Cell Biol. 107, pp851-863.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08903 pANY1-4 CEN URA3 Ap SEC12
RDB08904 pANY1-8 Int. Ap sec12Δ(274-C)::URA3::sec12Δ(N-47) Cut KpnI, then trafo
RDB08905 pANY1-9 URA3 Ap SEC12
RDB08916 pANF1 bacterial Ap lacZ-Sec12(48-Cterm) expression vector (lac promoter), antigen for Sec12p antibody

A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus. (1989) J Cell Biol. 109, pp2677-2691.
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08916 pANF1 bacterial Ap lacZ-Sec12(48-Cterm) expression vector (lac promoter), antigen for Sec12p antibody
RDB08906 pANY2-7 URA3 Ap SAR1 gemomic (intron at 10th codon)
RDB08907 pANY2-9 CEN URA3 Ap SAR1 gemomic (intron at 10th codon)
RDB08908 pANY2-17 CEN TRP1 Ap SAR1 gemomic (intron at 10th codon)
RDB08909 pANY2-18 CEN TRP1 Ap PGAL1-SAR1 gemomic (intron at 10th codon)
RDB08910 pANY2-19 TRP1 Ap PGAL1-SAR1 gemomic (intron at 10th codon)
RDB08911 pANY3-11 CEN TRP1 Ap PGAL1-SAR1 cDNA
RDB08912 pANY3-14 bacterial Ap SAR1 cloning vector, cDNA

The GTP-binding Sar1 protein is localized to the early compartment of the yeast secretory pathway. (1991) Biochim. Biophys. Acta. 1093, pp135-143
Catalog No. plasmid backborne Sc marker Ec marker insert remarks
RDB08875 pSHY1-1 TRP1 Ap SAR1 gemomic (intron at 10th codon)
RDB08913 pANF31 bacterial Ap lacZ-Sar1(42-190) expression vector (lac promoter), antigen for Sar1p antibody

(2014.05.09)

2023.06.16



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