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Mitsuhiro Abe's Resource

Learn more about their research in ReaD in English    in Japanese.

Mitsuhiro Abe's web page in English   in Japanese.

Catalog #Name of cloneRunning titlePublication
RDB13496pET28/EqtII-EGFP-His6Marker to localize sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II.Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. (original).
RDB13497pET28/EqtII(8-69)-EGFP-His6Marker to localize sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Eqinatoxin II.Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. (original).
RDB13498pET28/His6-EGFP-NT-LysMarker to localize sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. (original).
RDB13499pET28/His6-mCherry-NT-LysMarker to localize sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. (original).
RDB13501pCAG/tdiRFP-CAAXMembrane marker. Mammalian expression plasmid of CAAX box protein.Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. (original).
RDB13958pMAL/MBP-lyseninMarker to localize sphingomyelin in cell membrane. Bacterial expression plasmid of MBP-tagged lysenin.Kiyokawa, E., Recognition of sphingomyelin by lysenin and lysenin-related proteins. Biochemistry 43 (30): 9766-9773 (2004). PMID 15274631. (original).
RDB13959pQE30/His6-Venus-NT-LysMarker to localize sphingomyelin in cell membrane. Bacterial expression plasmid of His6-Venus-tagged non toxic lysenin.Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575. (original).
RDB13960pQE30/His6-mRFP-NT-LysMarker to localize sphingomyelin in cell membrane. Bacterial expression plasmid of His6-mRFP-tagged non toxic lysenin.Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575. (original).
RDB13961pET28/His6-EGFP-D4Marker to localize cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-EGFP-tagged domein 4 of theta toxin (D4).Shimada, Y., The C-terminal domain of perfringolysin O is an essential cholesterol-binding unit targeting to cholesterol-rich microdomains. Eur. J. Biochem. 269 (24): 6195-6203 (2002). PMID 12473115. (original).
RDB13962pET28/PlyA2-EGFP-His6Marker to localize sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged PlyA2.Bhat, H.B., Binding of a pleurotolysin ortholog from Pleurotus eryngii to sphingomyelin and cholesterol-rich membrane domains. J. Lipid Res. 54 (10): 2933-2943 (2013). PMID 23918047. (original).
RDB13963pET28/Oly-EGFP-His6Marker to localize sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged Oly.Bhat, H.B., Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates. FASEB J. 29 (9): 3920-3934 (2015). PMID 26060215. (original).
RDB13964pET28/EryA-EGFP-His6Marker to localize sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged EryA.Bhat, H.B., Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates. FASEB J. 29 (9): 3920-3934 (2015). PMID 26060215. (original).
RDB14300pET28/His6-mCherry-D4Marker to localize cholesterol-rich domain of the plasma membrane. Expression vector of His and mCherry-tagged C. perfringens perfringolysin theta derivatives encoding the D4 for E. coli.Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 2016 Aug 4. pii: fj.201500075R. [Epub ahead of print] PMID 27492925. (original).
RDB14301pET28/His6-EGFP-NakanoriMarker to localize sphingomyelin and cholesterol lipid domains. Expression vector of His and EGFP-tagged G. frondosa Nakaniori for E. coli.Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 2016 Aug 4. pii: fj.201500075R. [Epub ahead of print] PMID 27492925. (original).
RDB14697pSpCas9(BB)-2A-puro-ACAT1 sgRNAExpression vector of sgRNA for targeting human ACAT1 and Cas9.Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original).
RDB14698pSpCas9(BB)-2A-puro-ACAT2 sgRNAExpression vector of sgRNA for targeting human ACAT2 and Cas9.Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original).
RDB14699pDEST/mCherry-Rab18Expression vector of human Rab18, wild type, fused with mCherry.Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original).
RDB14700pDEST/mCherry-Rab18 Q67LExpression vector of human Rab18, constitutively active GTP-binding mutant (Q67L), fused with mCherry.Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original).
RDB14701pDEST/mCherry-Rab18 S22NExpression vector of human Rab18, dominant-negative GDP-binding mutant (S22N), fused with mCherry.Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original).

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2017.09.11

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