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Yasushi Sako's Resource

Learn more about their research in ReaD in English    in Japanese.

Yasushi Sako's web page in English   in Japanese.

Catalog #Name of cloneRunning titlePublication
RDB13254GL023Expression plasmid of GFP-tagged human c-RAF.Hibino K, A RasGTP-induced conformational change in C-RAF is essential for accurate molecular recognition. Biophys. J. 97 (5): 1277-1287 (2009). PMID 19720015. (original).
RDB13255GL348Expression plasmid of GFP-tagged human cRaf1 catalytic domain (CAD, 330-627)Hibino, K., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573. (original).
RDB13256GL207Expression plasmid of GFP-tagged human cRaf1 CRDCAD (R89A S621A).Hibino, K., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573. (original).
RDB13257GL107Expression plasmid of GFP-tagged human cRaf1 (R89A mutant).Hibino, K., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573. (original).
RDB14152pGEX-GFP-ERK2-HisBacterial expression vector of GFP–human ERK2.Shindo, Y., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866. (original).
RDB14154pACYC184-Venus-MEK1 (S218/222E, delta32-51)Plasmid clone of constitutive active form of human MEK1.Shindo, Y., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866. (original).
RDB14156pCMV-GFP-ERK2Expression vector of GFP-tagged human ERK2.Shindo, Y., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866. (original).
RDB14300pET28/His6-mCherry-D4Marker to localize cholesterol-rich domain of the plasma membrane. Expression vector of His and mCherry-tagged C. perfringens perfringolysin theta derivatives encoding the D4 for E. coli.Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 2016 Aug 4. pii: fj.201500075R. [Epub ahead of print] PMID 27492925. (original).
RDB14301pET28/His6-EGFP-NakanoriMarker to localize sphingomyelin and cholesterol lipid domains. Expression vector of His and EGFP-tagged G. frondosa Nakaniori for E. coli.Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 2016 Aug 4. pii: fj.201500075R. [Epub ahead of print] PMID 27492925. (original).
RDB14697pSpCas9(BB)-2A-puro-ACAT1 sgRNAExpression vector of sgRNA for targeting human ACAT1 and Cas9.Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original).
RDB14698pSpCas9(BB)-2A-puro-ACAT2 sgRNAExpression vector of sgRNA for targeting human ACAT2 and Cas9.Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original).
RDB14699pDEST/mCherry-Rab18Expression vector of human Rab18, wild type, fused with mCherry.Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original).
RDB14700pDEST/mCherry-Rab18 Q67LExpression vector of human Rab18, constitutively active GTP-binding mutant (Q67L), fused with mCherry.Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original).
RDB14701pDEST/mCherry-Rab18 S22NExpression vector of human Rab18, dominant-negative GDP-binding mutant (S22N), fused with mCherry.Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original).

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2017.06.20

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