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pcDNA5-GAPDH-HaloTag

Expression vector for the visualization of chaperone-mediated autophagy (CMA).

Catalog number RDB15088
Resource name pcDNA5-GAPDH-HaloTag
Alternative name GAPDH-HT/pcDNA5/FRT
Clone info. Expression vector of human glyceraldehyde 3-phosphate dehydrogenase (GAPDH), tagged with C-terminal HaloTag, for genomic integration. Translocation of the GAPDH-HT labeled with an appropriate fluorescent dye as a substrate of chaperone-mediated autophagy (CMA) from cytosol to lysosome can be detected. Refer Fig. 1 of Seki, T., et al., PLoS ONE 7 (2), e31232, 2012.
Expression was confirmed by the depositor with immunostaining.
[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB15088]
Vector backbone pcDNA5/FRT (Plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r (E. coli), Hyg^r (mammalian cells).
Gene/insert name Human GAPDH cDNA
Depositor Seki, Takahiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sato, M. et al. Genes Cells 21 (8): 861-873, 2016).
(Japanese text [open/close])
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15088 pcDNA5-GAPDH-HaloTag DNA solution

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

Featured content

Featured content Autophagy (English text)

Reference

original Sato, M., Fluorescent-based evaluation of chaperone-mediated autophagy and microautophagy activities in cultured cells. Genes Cells 21 (8): 861-873 (2016). PMID 27377049.
original Seki,T., Establishment of a novel fluorescence-based method to evaluate chaperone-mediated autophagy in a single neuron. PLoS One 7 (2): e31232 (2012). PMID 22363588.

2017.06.24

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