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pAcGFP1-N1-RvLEAM

Expression vector of GFP-fused water bear (Ramazzottius varieornatus) Late embryogenesis abundant from mitochondria (RvLEAM).

Catalog number RDB14656
Resource name pAcGFP1-N1-RvLEAM
Clone info. Expression vector of GFP-fused water bear (Ramazzottius varieornatus) Late embryogenesis abundant from mitochondria (RvLEAM) under the CMV promoter for transient expression in mammalian cells.
Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pAcGFP1-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Ramazzottius varieornatus RvLEAM cDNA
Reference of nucleotide sequence LC002821 (DDBJ accession number)

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Tanaka, S et al. PLoS One, 10(2): e0118272, 2015)
(Japanese text [open/close])
Remarks ((Remarks)) Please contact us. We will provide you with appropriate documents.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14656 pAcGFP1-N1-RvLEAM DNA solution Please contact us.

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

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References

original Tanaka, S., Novel mitochondria-targeted heat-soluble proteins identified in the anhydrobiotic Tardigrade improve osmotic tolerance of human cells. PLoS One 10 (2): e0118272 (2015). PMID 25675104.

2017.08.30

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