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pEThT-RvLEAM

Expression vector of N-terminus 6xHis-tagged water bear (Ramazzottius varieornatus) Late embryogenesis abundant from mitochondria (RvLEAM) in E. coli.

Catalog number RDB14654
Resource name pEThT-RvLEAM
Clone info. pET expression system vector of N-terminus 6xHis-tagged water bear (Ramazzottius varieornatus) Late embryogenesis abundant from mitochondria (RvLEAM) under the T7 promoter for expression recombinant protein in E. coli.
Expression was confirmed by the depositor with Heat-solubility assay.
Vector backbone pEThT (plasmid)
Selectable markers Amp^r
Gene/insert name Ramazzottius varieornatus RvLEAM cDNA
Reference of nucleotide sequence LC002821 (DDBJ accession number)
Depositor Kunieda, Takekazu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Tanaka, S et al. PLoS One, 10(2): e0118272, 2015)
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14654 pEThT-RvLEAM DNA solution

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

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References

original Tanaka, S., Novel mitochondria-targeted heat-soluble proteins identified in the anhydrobiotic Tardigrade improve osmotic tolerance of human cells. PLoS One 10 (2): e0118272 (2015). PMID 25675104.

2017.08.30

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