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pEThT-MAHS delta MTP

Expression vector of N-terminus 6xHis-tagged water bear (Ramazzottius varieornatus) Mitochondrial Abundant Heat Soluble (MAHS) without mitochondia targeting peptide in E. coli.

Catalog number RDB14653
Resource name pEThT-MAHS delta MTP
Clone info. pET expression system vector of N-terminus 6xHis-tagged water bear (Ramazzottius varieornatus) Mitochondrial Abundant Heat Soluble (MAHS) without mitochondia targeting peptide under the T7 promoter for expression recombinant protein in E. coli.
Expression was confirmed by the depositor with Heat-solubility assay.
Vector backbone pEThT (plasmid)
Selectable markers Amp^r
Gene/insert name Ramazzottius varieornatus MAHS cDNA
Reference of nucleotide sequence LC002822 (DDBJ accession number)
Depositor Kunieda, Takekazu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Tanaka, S et al. PLoS One, 10(2): e0118272, 2015)
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14653 pEThT-MAHS delta MTP DNA solution

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

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References

original Tanaka, S., Novel mitochondria-targeted heat-soluble proteins identified in the anhydrobiotic Tardigrade improve osmotic tolerance of human cells. PLoS One 10 (2): e0118272 (2015). PMID 25675104.

2017.08.30

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