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pEThT-CAHS3

Expression vector of N-terminus 6xHis-tagged water bear (Ramazzottius varieornatus) Cytoplasmic Abundant Heat Soluble 3 (CAHS3) in E. coli.

Catalog number RDB14649
Resource name pEThT-CAHS3
Clone info. pET expression system vector of N-terminus 6xHis-tagged water bear (Ramazzottius varieornatus) Cytoplasmic Abundant Heat Soluble 3 (CAHS3) under the T7 promoter for expression recombinant protein in E. coli.
Expression was confirmed by the depositor with SDS-PAGE and CD spectra.
Vector backbone pEThT (plasmid)
Selectable markers Amp^r
Gene/insert name Ramazzottius varieornatus CAHS3 cDNA
Reference of nucleotide sequence AB650501 (DDBJ accession number)
Depositor Kunieda, Takekazu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Yamaguchi, A et al. PLoS One, 7(8): e44209, 2012)
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14649 pEThT-CAHS3 DNA solution

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

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References

original Yamaguchi, A., Two novel heat-soluble protein families abundantly expressed in an anhydrobiotic tardigrade. PLoS One 7 (8): e44209 (2012). PMID 22937162.

2017.08.30

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