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pEThT-SAHS1

Expression vector of N-terminus 6xHis-tagged water bear (Ramazzottius varieornatus) Secretory Abundant Heat Soluble 1 (SAHS1) in E. coli.

Catalog number RDB14646
Resource name pEThT-SAHS1
Clone info. pET expression system vector of N-terminus 6xHis-tagged water bear (Ramazzottius varieornatus) Secretory Abundant Heat Soluble 1 (SAHS1) under the T7 promoter for expression recombinant protein in E. coli.
Expression was confirmed by the depositor with SDS-PAGE and CD spectra.
Vector backbone pEThT (plasmid)
Selectable markers Amp^r
Gene/insert name Ramazzottius varieornatus SAHS1 cDNA
Reference of nucleotide sequence AB650497 (DDBJ accession number)

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Yamaguchi, A et al. PLoS One, 7(8): e44209, 2012)
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14646 pEThT-SAHS1 DNA solution

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

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References

original Yamaguchi, A., Two novel heat-soluble protein families abundantly expressed in an anhydrobiotic tardigrade. PLoS One 7 (8): e44209 (2012). PMID 22937162.

2017.08.30

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