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G-CaMP-HS

Genetically-encoded fluorescent calcium sensor cDNA, G-CaMP-HS.

Catalog number RDB14607
Resource name G-CaMP-HS
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. GCaMP-hyper sensitive (G-CaMP-HS) protein became more stable and stronger fluorescence than GCaMP2 in transfected mammal cells, and a GCaMP-HS is better suited to detect Ca2+ transients in vivo imaging of neuronal activities in zebrafish.

Expression was confirmed by the depositor with calcium imaging.
[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB14607]
Vector backbone pEGFP-N1
Size of vector backbone 4.7 kb
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Classification plasmid
Type_of_insert fragment Rat cDNA ()
Reference of nucleotide sequence AKE44628.1 (DDBJ accession number)
Deposotor's page Nakai, Junichi | Ohkura, Masamichi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Muto, A. et al. Proc. Nati. Acad. Sci. USA 108 (13): 5425-5430, 2011) and an acknowledgment to the DEPOSITOR are requested. The DEPOOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
(Japanese text [open/close])
Remarks ((Additional form)) GFP-A
(Japanese text [open/close])
Fee (non-profit org.) Please contact us.

Sequence information

References and tips

Electronic file

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Featured content G-CaMP Clones (English text)

Reference

original Muto, A., Genetic visualization with an improved GCaMP calcium indicator reveals spatiotemporal activation of the spinal motor neurons in zebrafish. Proc. Natl. Acad. Sci. U.S.A. 108 (13): 5425-5430 (2011). PMID 21383146.

2017.03.07

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