Expression vector human Transient receptor potential vanilloid type I (TRPV1) wild type with C-terminal FLAG-tag.
|Resource name||pcDNA3.1-hTRPV1-FLAG WT|
|Clone info.||Expression vector human Transient receptor potential vanilloid type I (TRPV1) wild type with C-terminal FLAG-tag.
Espression was confirmed by the depositor with Ca2+ flux assay.
[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB14587]
|Size of vector backbone||5.4 kb|
|Selectable markers||Amp^r (E. coli), Neo^r (mammalian cells).|
|Type_of_insert fragment||human cDNA (systhetic DNA)|
|Deposotor's page||, ||
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
|Terms and conditions set forth by the DEPOSITOR||In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ohbuchi, K. et al. PLoS One., 11 (9): e0162543, 2016)|
(Japanese text [open/close])
利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ohbuchi, K. et al. PLoS One., 11 (9): e0162543, 2016)。
|Fee (non-profit org.)|
This clone will be sequenced a portion and digested by restriction enzyme for examination.
|original||Ohbuchi, K., Detailed Analysis of the Binding Mode of Vanilloids to Transient Receptor Potential Vanilloid Type I (TRPV1) by a Mutational and Computational Study. PLoS One 11 (9): e0162543 (2016). PMID 27606946.|