Resource data sheet
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pAcGFP1-N1-Dsup

Expression vector of GFP-fused Ramazzottius varieornatus Dsup.

Catalog number RDB14552
Resource name pAcGFP1-N1-Dsup
Clone info. Expression vector of GFP-fused Ramazzottius varieornatus Dsup under the CMV promoter for transient expression in mammalian cells.
Expression was confirmed by the depositor with immunohistochemistry.
[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB14552]
Vector backbone pAcGFP1-N1
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Classification Plasmid
Type_of_insert fragment Ramazzottius varieornatus cDNA ()
Reference of nucleotide sequence LC050827 (DDBJ accession number)

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Hashimoto, T. et al. Nature Commu. 7, 12808, 2016).
(Japanese text [open/close])
Remarks ((Remarks)) Please contact us. We will provide you with appropriate documents.
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Fee (non-profit org.)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

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Reference

original Hashimoto, , Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nat. Commun. 7: 12808 (2016). PMID 27649274.

2017.03.17

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