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pAcGFP1-N1-Dsup

Expression vector of GFP-fused Ramazzottius varieornatus Dsup.

Catalog number RDB14552
Resource name pAcGFP1-N1-Dsup
Clone info. Expression vector of GFP-fused Ramazzottius varieornatus Dsup under the CMV promoter for transient expression in mammalian cells.
Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pAcGFP1-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Ramazzottius varieornatus Dsup cDNA
Reference of nucleotide sequence LC050827 (DDBJ accession number)
Depositor Kunieda, Takekazu | Hashimoto, Takuma |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Hashimoto, T. et al. Nature Commu. 7, 12808, 2016).
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Remarks ((Remarks)) Please contact us. We will provide you with appropriate documents.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14552 pAcGFP1-N1-Dsup DNA solution Please contact us.

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

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References

original Hashimoto, , Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nat. Commun. 7: 12808 (2016). PMID 27649274.

2017.09.30

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