Resource data sheet

BL21(DE3)-based RFzero-iy

Escherichia coli host strain to produce non-natural amino acids containing proteins.

Catalog number RDB14428
Resource name BL21(DE3)-based RFzero-iy
Clone info. Escherichia coli BL21(DE3)-based host strain with the UAG codon reassigned to 3-iodotyrosine.
BL21[DE3]: F-, dcm, ompT, hsdS(rB-mB-), gal, (LambdaDE3); RFzero-iy: prfA::zeo, BAC8(cat), piodoTyrRS-MJR1-gent(pACYC)
Vector backbone Bacteria culture
Depositor Sakamoto, Kensaku |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Mukai, T., Biochem. Biophys. Res. Commun. 411(4):757-761, 2011) and an acknowledgment to the DEPOSITOR is requested. The materials are only available based on material transfer agreement between the user and the depositor. The materials are only available for the user belonging to a nonprofit organization.
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Remarks ((Additional form)) Approval Form(FormD)
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14428 BL21(DE3)-based RFzero-iy Bacteria in stub agar

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

Electronic file PDF RDB14428z.pdf from Depositor

Featured content

Featured content Bacterial host strains (English text)
Featured content Incorporation of synthetic amino acids into proteins at specific sites (English text)
Featured content Incorporation of synthetic amino acids into proteins at specific sites (Japanese text)


original Mukai, T., Genetic-code evolution for protein synthesis with non-natural amino acids. Biochem. Biophys. Res. Commun. 411 (4): 757-761 (2011). PMID 21782790.
reference Ohtake, K., Protein stabilization utilizing a redefined codon. Sci. Rep. 5: 9762 (2015). PMID 25985257.
reference Ohtake, K., Efficient decoding of the UAG triplet as a full-fledged sense codon enhances the growth of a prfA-deficient strain of Escherichia coli. J. Bacteriol. 194 (10): 2606-2613 (2012). PMID 22427623.
reference Mukai, T., Codon reassignment in the Escherichia coli genetic code. Nucleic Acids Res. 38 (22): 8188-8195 (2010). PMID 20702426.