Resource data sheet
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pCoGFP-V4

Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa.

Catalog number RDB14374
Resource name pCoGFP-V4
Clone info. Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa. Variant-4 (Nucleotide sequence accession number: AB743887). pCoGFP-V4 is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Cavernularia obesa GFP cDNA
Reference of nucleotide sequence AB743887 (DDBJ accession number)
Depositor OLYMPUS CORPORATION, | Ogoh, Katsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Ogoh, K. Luminescence 28 (4): 582-591 (2013)). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14374 pCoGFP-V4 DNA solution Please contact us.

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14374_A6Jcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2 RDB14374_A6Jca.seq
>D03707B7_A6Jc_1_Reverse2_H05_23_ABI24.ab1
    1 CGTTAAATCA ATTACGGCCA GCTTGCATGC CTGCAGGTCG ACTCTAGAGG ATCCGATGAG
   61 CATCCCCGAG AACAGCGGCC TGACCGAAGA GATGCCCGCC CAGATGAACC TGGAAGGGGT
  121 GGTCAACGGC CACGCCTTCT CCATGGAAGG CATCGGCGGA GGCAACATCC TGACCGGCAT
  181 CCAGAAACTG GACATCAGAG TGATCGAGGG CGACCCCCTG CCCTTCAGCT TTGACATCCT
  241 GAGCGTGGCC TTCCAGTACG GCAACCGGAC CTACACCAGC TACCCCGCCA AGATCCCCGA
  301 CTACTTCGTG CAGAGCTTCC CCGAGGGCTT CACCTTCGAG CGGACCCTGA GCTTTGAGGA
  361 CGGCGCCATC GTGAAGGTGG AAAGCGACAT CAGCATCGAG GACGGCAAGT TCGTGGGCAA
  421 GATCAAGTAC AACGGCGAGG GCTTCCCAGA GGACGGCCCC GTGATGAAGA AAGAAGTGAC
  481 CAAGCTGGAA CCCGCCAGCG AGTCTATGTA CGTGAGCGAC GGCACCCTCG TGGGCGAGGT
  541 GGTGCTGAGC TACAAGACCC AGAGCACCCA CTACACCTGT CACATGAAGA CCATCTACCG
  601 GTCCAAGAAA CCCGTGGAAA ACCTGCCCAA GTTCCACTAC GTGCACCACC GGCTCGAAAA
  661 GAAAATCGTC GAAGAGGGCT ACTACTACGA GCAGCACGAG ACAGCCATTG CCAAGCCCTG
  721 AGAATTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC
  781 TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA
  841 CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCCTG ATGCGGTATT
  901 TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC AGTACAATCT
  961 GCTCTGATGC CCGCATAGTT AAGCCCAGCC CCCGACACCC GCCCAACACC CGGCTTGACG
 1021 GCCGCCCTGA CGGCTTGTCT GGCTTCCGGC CATTCGCCTT ACAGACAGCC TGTGAACGGT
1081 CTCCGGAAGC TGGCATGGTG GTTCCAG
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Higher intensity luciferase and pH sensor GFP (Japanese text)
Featured content Higher intensity luciferase and pH sensor GFP (English text)

References

original Ogoh, K., Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591 (2013). PMID 23468077.

2017.08.30

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