Resource data sheet
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pCoGFP-V3

Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa.

Catalog number RDB14373
Resource name pCoGFP-V3
Clone info. Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa. Variant-3 (Nucleotide sequence accession number: AB743886). pCoGFP-V3 is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Cavernularia obesa GFP cDNA
Reference of nucleotide sequence AB743886 (DDBJ accession number)
Depositor OLYMPUS CORPORATION, | Ogoh, Katsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Ogoh, K. Luminescence 28 (4): 582-591 (2013)). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14373 pCoGFP-V3 DNA solution Please contact us.

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14373_A6Jcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2 RDB14373_A6Jca.seq
>D03707B6_A6Jc_1_Reverse2_F05_17_ABI24.ab1
    1 CGGTAAAGGA ATTACGCCAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCGATGAGC
   61 ATCCCCGAGA ACAGCGGCCT GACCGAAGAG ATGCCCGCCC AGATGAACCT GGAAGGGGTG
  121 GTCAACGGCC ACGCCTTCTC CATGGAAGGC ATCGGCGGAG GCAACATCCT GACCGGCATC
  181 CAGAAACTGG ACATCAGAGT GATCGAGGGC GACCCCCTGC CCTTCAGCTT TGACATCCTG
  241 AGCGTGGCCT TCCAGTACGG CAACCGGACC TACACCAGCT ACCCCGCCAA GATCCCCGAC
  301 TACTTCGTGC AGAGCTTCCC CGAGGGCTTC ACCTTCGAGC GGACCCTGAG CTTTGAGGAC
  361 GGCGCCATCG TGAAGGTGGA AAGCGACATC AGCATCGAGG ACGGCAAGTT CGTGGGCAAG
  421 ATCAAGTACA ACGGCGAGGG CTTCCCAGAG GACGGCCCCG TGATGAAGAA AGAAGTGACC
  481 AAGCTGGAAC CCTGCAGCGA GTCTATGTAC GTGAGCGACG GCACCCTCGT GGGCGAGGTG
  541 GTGCTGAGCT ACAAGACCCA GAGCACCCAC TACACCTGTC ACATGAAGAC CATCTACCGG
  601 TCCAAGAAAC CCGTGGAAAA CCTGCCCAAG TTCCACTACG TGCACCACCG GCTCGAAAAG
  661 AAAATCGTCG AAGAGGGCTA CTACTACGAG CAGCACGAGA CAGCCATTGC CAAGCCCTGA
  721 GAATTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT
  781 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC
  841 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCCTGA TGCGGTATTT
  901 TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG
  961 CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCC AACACCCGCT GACGCGCCCT
 1021 GACGGGGCTT GTCTGCCTCC GGCCATCCGC TTACAGACAG CCTGTGAACC GGATCTCCGG
1081 GAGCTTGCAT GGGTGATA
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Higher intensity luciferase and pH sensor GFP (Japanese text)
Featured content Higher intensity luciferase and pH sensor GFP (English text)

References

original Ogoh, K., Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591 (2013). PMID 23468077.

2017.08.30

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