Resource data sheet
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pCoGFP-V2

Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa.

Catalog number RDB14372
Resource name pCoGFP-V2
Clone info. Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa. Variant-2 (Nucleotide sequence accession number: AB743885). pCoGFP-V2 is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Cavernularia obesa GFP cDNA
Reference of nucleotide sequence AB743885 (DDBJ accession number)
Depositor OLYMPUS CORPORATION, | Ogoh, Katsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Ogoh, K. Luminescence 28 (4): 582-591 (2013)). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14372 pCoGFP-V2 DNA solution Please contact us.

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14372_A6Jcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2 RDB14372_A6Jca.seq
>D03707B5_A6Jc_2_Reverse2_D05_11_ABI24.ab1
    1 CATACATCAT TACGCCAGCT TGCATGCCTG CAGGTCGACT CTAGAGGATC CGATGAGCAT
   61 CCCCGAGAAC AGCGGCCTGA CCGAAGAGAT GCCCGCCCAG ATGAACCTGG AAGGGGTGGT
  121 CAACGGCCAC GCCTTCTCCA TGGAAGGCAT CGGCGGAGGC AACATCCTGA CCGGCATCCA
  181 GAAACTGGAC ATCAGAGTGA TCGAGGGCGA CCCCCTGCCC TTCAGCTTTG ACATCCTGAG
  241 CGTGGCCTTC CAGTACGGCA ACCGGACCTA CACCAGCTAC CCCGCCAAGA TCCCCGACTA
  301 CTTCGTGCAG AGCTTCCCCG AGGGCTTCAC CTTCGAGCGG ACCCTGAGCT TTGAGGACGG
  361 CGCCATCGTG AAGGTGGAAA GCGACATCAG CATCGAGGAC GGCAAGTTCG TGGGCAAGAT
  421 CAAGTACAAC GGCGAGGGCT TCCCAGAGGA CGGCCCCGTG ATGAAGAAAG AAGTGACCAA
  481 GCTGGAACCC GGCAGCGAGT CTATGTACGT GAGCGACGGC ACCCTCGTGG GCGAGGTGGT
  541 GCTGAGCTAC AAGACCCAGA GCACCCACTA CACCTGTCAC ATGAAGACCA TCTACCGGTC
  601 CAAGAAACCC GTGGAAAACC TGCCCAAGTT CCACTACGTG CACCACCGGC TCGAAAAGAA
  661 AATCGTCGAA GAGGGCTACT ACTACGAGCA GCACGAGACA GCCATTGCCA AGCCCTGAGA
  721 ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA
  781 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG
  841 ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCCTGATG CGGTATTTTC
  901 TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG CACTCTCAGT ACAATCTGCT
  961 CTGATGCCGC ATAGTTTAAG CCAGCCCCGA CACCCGGCCC AACACCCGGC TGACGCGCCC
 1021 TGACGGGGCT TTGGTCTGCT CCGGCATCGG CTTAACAGGA TAGCCTGTGA ACGATCTCCG
1081 GGAGCTGCAT GGGTGTGTTC CA
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Higher intensity luciferase and pH sensor GFP (Japanese text)
Featured content Higher intensity luciferase and pH sensor GFP (English text)

References

original Ogoh, K., Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591 (2013). PMID 23468077.

2017.08.30

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