Resource data sheet
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pCoGFP-V1

Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa.

Catalog number RDB14371
Resource name pCoGFP-V1
Clone info. Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa. Variant-1 (Nucleotide sequence accession number: AB743884). pCoGFP-V1 is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Cavernularia obesa GFP cDNA
Reference of nucleotide sequence AB743884 (DDBJ accession number)
Depositor OLYMPUS CORPORATION, | Ogoh, Katsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Ogoh, K. Luminescence 28 (4): 582-591 (2013)). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14371 pCoGFP-V1 DNA solution Please contact us.

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14371_A6Jcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2 RDB14371_A6Jca.seq
>D03707B4_A6Jc_3_Reverse2_B05_05_ABI24.ab1
    1 CATCATCATT ACGCCAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC GATGAGCATC
   61 CCCGAGAACA GCGGCCTGAC CGAAGAGATG CCCGCCCAGA TGAACCTGGA AGGGGTGGTC
  121 AACGGCCACG CCTTCTCCAT GGAAGGCATC GGCGGAGGCA ACATCCTGAC CGGCATCCAG
  181 AAACTGGACA TCAGAGTGAT CGAGGGCGAC CCCCTGCCCT TCAGCTTTGA CATCCTGAGC
  241 GTGGCCTTCC AGTACGGCAA CCGGACCTAC ACCAGCTACC CCGCCAAGAT CCCCGACTAC
  301 TTCGTGCAGA GCTTCCCCGA GGGCTTCACC TTCGAGCGGA CCCTGAGCTT TGAGGACGGC
  361 GCCATCGTGA AGGTGGAAAG CGACATCAGC ATCGAGGACG GCAAGTTCGT GGGCAAGATC
  421 AAGTACAACG GCGAGGGCTT CCCAGAGGAC GGCCCCGTGA TGAAGAAAGA AGTGACCAAG
  481 CTGGAACCCA CCAGCGAGTC TATGTACGTG AGCGACGGCA CCCTCGTGGG CGAGGTGGTG
  541 CTGAGCTACA AGACCCAGAG CACCCACTAC ACCTGTCACA TGAAGACCAT CTACCGGTCC
  601 AAGAAACCCG TGGAAAACCT GCCCAAGTTC CACTACGTGC ACCACCGGCT CGAAAAGAAA
  661 ATCGTCGAAG AGGGCTACTA CTACGAGCAG CACGAGACAG CCATTGCCAA GCCCTGAGAA
  721 TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA
  781 TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA
  841 TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCCTGATGC GGTATTTTCT
  901 CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC
  961 TGATGCCGCA TAGTTTAAGC CAGCCCCGAC ACCCGGCGAC ACCCGCTGAC GCGCCTGACG
 1021 GCTGTCTGCT CCTGCATCGG CTACAGACAG CTGTGACCGT CTCAGGGAGC CTGCCATGTG
1081 TTCCAGAAGT
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Higher intensity luciferase and pH sensor GFP (Japanese text)
Featured content Higher intensity luciferase and pH sensor GFP (English text)

References

original Ogoh, K., Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591 (2013). PMID 23468077.

2017.08.30

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