Resource data sheet
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pCoGFP-wt

Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa.

Catalog number RDB14368
Resource name pCoGFP-wt
Clone info. Dual-color-emitting green fluorescent protein of sea cactus Cavernularia obesa. Wild-type (Nucleotide sequence accession number: AB743881). pCoGFP-wt is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Cavernularia obesa GFP cDNA
Reference of nucleotide sequence AB743881 (DDBJ accession number)
Depositor OLYMPUS CORPORATION, | Ogoh, Katsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Ogoh, K. Luminescence 28 (4): 582-591 (2013)). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14368 pCoGFP-wt DNA solution Please contact us.

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14368_A6Jcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB14368_A6Jca.seq
>D03707B1_A6Jc_2_pCU_M13-100_C02_08_ABI24.ab1
    1 GACGGTAAGG CGATTAGTTG GGTACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA
   61 CGGCCAGTGA ATTCGAGCTC GGTACCTCAT GGTTTAGCTA TAGCCGTCTC ATGTTGCTCG
  121 TAGTAGTTGC CTTCCTCGAC TTTTTTCTTT TCGAGGCGAT GATGAACATA ATGGAACTTT
  181 GGCAGGTTTT CTACTGGCTT TTTGGACCTG TAGATTGTTT TCATATGGCA AGTGTAATGA
  241 GTCGACTGGG TCTTGTAAGA CAACACAACT TCTCCCACCA AAGTGTCGTC ACATACATAC
  301 ATTGACTCAC TGGACGGTTC TAGTTTGGTA ACTTCCTTTT TCATCACCGG TCCATCCTCT
  361 GGAAAACTTT CGCCATTATA TTTAATCTTG CCAACAAATT TGCCATCTTC AATACTAATA
  421 TCCGATTCGA CCTTTACGAT TGCACCATCT TCAAAACTAA GAGTCCTTTC AAAGGTGAAC
  481 CCTTCTGGGA ATGATTGAAC GAAATAATCG GGGATCTTCG CAGGATAACT TGTGTAAGTT
  541 CTGTTGCCAT ATTGAAACGC AACTGAGAGA ATGTCAAAGG AAAACGGTAG AGGATCCCCC
  601 TCGATGACAC GGATATCCAG CTTTTGAATT CCAGTTAGTA TGTTCCCTCC ACCAATTCCT
  661 TCCATAGAGA AGGCATGACC ATTTACCACT CCTTCTAAAT TCATTTGAGC AGGCATCTCT
  721 TCTGTTAAGC CCGAATTCTC TGGAATACTC ATAAGCTTGG CGTAATCATG GTCATAGCTG
  781 TTTCCTGTGT GAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA
  841 AGTGTAAAGC CTGGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA
  901 CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTTAATGAA ATCGGCCAAC
  961 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCCTCGCT CACTGACTCG
 1021 CTGCGCTCGG TCGGTTCGGC TGCGGGCGAG CGGTATCCAG CCTCACTCAA AGGCGGTAAT
1081 ACGCTAATCA CAGGAGTCAA GGGGATTACG TCAAGGAAAA GGAATCCAG
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Higher intensity luciferase and pH sensor GFP (Japanese text)
Featured content Higher intensity luciferase and pH sensor GFP (English text)

References

original Ogoh, K., Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591 (2013). PMID 23468077.

2017.08.30

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