Resource data sheet
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pCoGFP-Th2co

Thermostable green fluorescent protein of sea cactus Cavernularia obesa.

Catalog number RDB14367
Resource name pCoGFP-Th2co
Clone info. Thermostable green fluorescent protein of sea cactus Cavernularia obesa. Mutant 2 codon optimized. pCoGFP-Th2co is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Cavernularia obesa GFP cDNA
Depositor OLYMPUS CORPORATION, | Ogoh, Katsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Murai M, Ogoh K, Kinebuchi T, Suzuki H. Thermostable fluorescent protein and its utility. Japan patent unregistered 2014-60947). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14367 pCoGFP-Th2co DNA solution Please contact us.

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14367_A6Jcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2 RDB14367_A6Jca.seq
>D03707A9_A6Jc_1_Reverse2_B02_05_ABI24.ab1
    1 CGGAAAGCAT TACGCCAGCT TGCATGCCTG CAGGTCGACT CTAGAGGATC CGATGAGCAT
   61 CCCCGAGAAC AGCGGCCTGA CCGAAGAGAT GCCCGCCCAG ATGAACCTGG AAGGGGTGGT
  121 CAACGGCCAC GCCTTCTCCA TGGAAGGCAT CGGCGGAGGC AACATCCTGA CCGGCATCCA
  181 GAAACTGGAC ATCAGAGTGA CCGAGGGCGA CCCCCTGCCC TTCAGCTTTG ACATCCTGAG
  241 CGTGGCCTTC CAGTACGGCA ACCGGACCTA CACCAGCTAC CCCGCCAAGA TCCCCGACTA
  301 CTTCGTGCAG AGCTTCCCCG AGGGCTTCAC CTTCGAGCGG ACCCTGAGCT TTGAGGACGG
  361 CGCCATCGTG AAGGTGGAAA GCGACATCAG CATCGAGGAC GGCAAGTTCG TGGGCAAGAT
  421 CAAGTACAAC GGCGAGGGCT TCCCAGAGGA CGGCCCCGTG ATGAAGAAAG AAGTGACCAA
  481 GCTGGAACCC AGCAGCGAGT CTATGTACGT GAGCGACGGC ACCCTCGTGG GCGAGGTGGT
  541 GCTGAGCTAC AAGACCCAGA GCACCCACTA CACCTGTCAC ATGAAGACCA TCTACCGGTC
  601 CAAGAAACCC GTGGAAAACC TGCCCAAGTT CCACTACGTG CACCACCGGC TCGAAAAGAA
  661 AATCGTCGAA GAGGGCTACT ACTACGAGCA GCACGAGACA GCCATTGCCA AGCCCTGAGA
  721 ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA
  781 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG
  841 ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCCTGATG CGGTATTTTC
  901 TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG CACTCTCAGT ACAATCTGCT
  961 CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCCAA CACCCGCTGA CGCGGCCCTG
 1021 ACGGGCTTTG TCTGCTCCGG CCATCCGGCT TTACAGACAG CTGTGACGTC TCCGGCAAGC
1081 TGCATGTGTT CAGGAGGATT TTTCAACG
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Higher intensity luciferase and pH sensor GFP (Japanese text)
Featured content Higher intensity luciferase and pH sensor GFP (English text)

References

original Murai, M., Thermostable fluorescent protein and its utility. Japan patent unregistered 2014-60947

2017.08.30

GNP_filter3_RDBDEP_html_170829.pl