Resource data sheet
Prev.

pCoGFP-Th2wt

Thermostable green fluorescent protein of sea cactus Cavernularia obesa. Mutant 2.

Catalog number RDB14366
Resource name pCoGFP-Th2wt
Clone info. Thermostable green fluorescent protein of sea cactus Cavernularia obesa. Mutant 2. pCoGFP-Th2wt is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Cavernularia obesa GFP cDNA
Depositor OLYMPUS CORPORATION, | Ogoh, Katsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Murai M, Ogoh K, Kinebuchi T, Suzuki H. Thermostable fluorescent protein and its utility. Japan patent unregistered 2014-60947). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14366 pCoGFP-Th2wt DNA solution Please contact us.

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14366_A6Jcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: AFR RDB14366_A6Jca.seq
>D03707A8_A6Jc_2_AFR_D12_12_ABI24.ab1
    1 CTCACGCTAT GACCATGATT ACGCCAAGCT TTCATGGTTT AGCTATGGCC GTCTCATGTT
   61 GCTCGTAGTA GTAGCCTTCC TCGACTATTT TCTTTTCGAG GCGATGATGA ACATAATGGA
  121 ACTTTGGCAG GTTTTCTACT GGCTTTTTGG ACCTGTAGAT TGTTTTCATA TGGCAAGTGT
  181 AATGAGTCGA CTGGGTCTTG TAAGACAACA CAACTTCTCC CACCAAAGTG CCGTCCGATA
  241 CATACATTGA CTCACTGGAC GGTTCTAGTT TGGTAACTTC CTTTTTCATC ACCGGTCCAT
  301 CCTCTGGAAA ACCTTCGCCA TTATATTTAA TCTTGCCAAC AAATTTGCCA TCTTCAATAC
  361 TAATATCCGA TTCGACCTTT ACGATTGCAC CATCTTCAAA ACTAAGAGTC CTTTCAAAGG
  421 TGAACCCTTC TGGGAATGAT TGAACGAAAT AATCGGGGAT CTTCGCAGGA TAACTTGTGT
  481 AAGTTCTGTT GCCATATTGA AACGCAACTG AGAGAATGTC AAAGGAAAAC GGAAGAGGAT
  541 CTCCCTCGGT GACACGGATA TCCAGCTTTT GAATTCCAGT TAGTATGTTC CCTCCACCAA
  601 TTCCTTCCAT AGAGAAGGCA TGACCATTTA CCACTCCTTC TAAATTCATT TGAGCAGGCA
  661 TCTCTTCTGT TAAGCCCGAA TTCTCTGGAA TACTCATGGA TCCCCGGGTA CCGAGCTCGA
  721 ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA
  781 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG
  841 ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCCTGATG CGGTATTTTC
  901 TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG CACTCTCAGT ACAATCTGCT
  961 CTGATGCCGC ATAGTTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA
 1021 CGGGCTTGTC TGCTCCCGGG CATCCGCTTA CAGACAGCTG TGACGTCTCC GGAGCTGCAT
 1081 GTGTCAGAGA TTTCACGTCA TCACCGAACG CGCGGACGAA CGACTTCGTG AATACGCTAT
1141 TTTTTAATAG GATTAATTGT TCCATGGATA AT
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Higher intensity luciferase and pH sensor GFP (Japanese text)
Featured content Higher intensity luciferase and pH sensor GFP (English text)

References

original Murai, M., Thermostable fluorescent protein and its utility. Japan patent unregistered 2014-60947

2017.08.30

GNP_filter3_RDBDEP_html_170829.pl