Resource data sheet
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pCoGFP-Th1wt

Thermostable green fluorescent protein of sea cactus Cavernularia obesa. Mutant 1.

Catalog number RDB14364
Resource name pCoGFP-Th1wt
Clone info. Thermostable green fluorescent protein of sea cactus Cavernularia obesa. Mutant 1. pCoGFP-Th1wt is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Cavernularia obesa GFP cDNA
Depositor OLYMPUS CORPORATION, | Ogoh, Katsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Murai M, Ogoh K, Kinebuchi T, Suzuki H. Thermostable fluorescent protein and its utility. Japan patent unregistered 2014-60947). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14364 pCoGFP-Th1wt DNA solution Please contact us.

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14364_A6Jcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 RDB14364_A6Jca.seq
>D03707A6_A6Jc_1_pCU_M13-100_C01_07_ABI24.ab1
    1 GTATGTCAAG GCGGATTAAG TTGGGTACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA
   61 CGACGGCCAG TGAATTCGAG CTCGGTACCC GGGGATCCAT GAGTATTCCA GAGAATTCGG
  121 GCTTAACAGA AGAGATGCCT GCTCAAATGA ATTTAGAAGG AGTGGTAAAT GGTCATGCCT
  181 TCTCTATGGA AGGAATTGGT GGAGGGAACA TACTAACTGG AATTCAAAAG CTGGATATCC
  241 GTGTCACCGA GGGAGATCCT CTTCCGTTTT CCTTTGACAT TCTCTCAGTT GCGTTTCAAT
  301 ATGGCAACAG AACTTACACA AGTTATCCTG CGAAGATCCC CGATTATTTC GTTCAATCAT
  361 TCCCAGAAGG GTTCACCTTT GAAAGGACTC TTAGTTTTGA AGATGGTGCA ATCGTAAAGG
  421 TCGAATCGGA TATTAGTATT GAAGATGGCA AATTTGTTGG CAAGATTAAA TATAATGGCG
  481 AAAGTTTTCC AGAGGATGGA CCGGTGATGA AAAAGGAAGT TATCAAACTA GAACCGTCCA
  541 GTGAGTCAAT GTATGTACGT GACGACACTT TGGTGGGAGA AGTTGTGTTG TCTTACAAGA
  601 CCCAGTCGAC TCATTACACT TGCCATATGA AAACAATCTA CAGGTCCAAA AAGCCAGTAG
  661 AAAACCTGCC AAAGTTCCAT TATGTTCATC ATCGCCTCGA AAAGAAAAAA GTCGAGGAAG
  721 GCAACTACTA CGAGCAACAT GAGACGGCCA TAGCTAAACC ATGAAAGCTT GGCGTAATCA
  781 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA
  841 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT
  901 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCCAGC TGCATTAATG
  961 AATCGGCCAA CGCGCGGGGG AGAGGCGGTT TGCGTATTGG GCCGCTCCTT CCGCCTTCCT
 1021 CGCTCACTGA CCTCGCCTGC CGCCTCCGGT CCGTTCCGGC CTGCCGCCGA GCCGATATCC
 1081 AAGCCTCCCA ACTCCAAGCC GTATACCGGT TTATTCCCAC ACAGAAGTTC CAGGGGGGGA
1141 TAC
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Higher intensity luciferase and pH sensor GFP (Japanese text)
Featured content Higher intensity luciferase and pH sensor GFP (English text)

References

original Murai, M., Thermostable fluorescent protein and its utility. Japan patent unregistered 2014-60947

2017.08.30

GNP_filter3_RDBDEP_html_170829.pl