Resource data sheet
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pMALuci2Luc

Green-shifted luciferase of Malaysian Luciola firefly with higher luminous intensity.

Catalog number RDB14363
Resource name pMALuci2Luc
Clone info. Green-shifted luciferase of Malaysian Luciola firefly with higher luminous intensity. A maximum luminescent wavelength falls 557 nm at pH8 and within a range of 555 to 560 nm over the pH range of 5.5 to 8.0 (Fig. 1 of patent application publication US 2015/0291938 A1). pMALuci2Luc is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
The luciferase gene originated from Malaysian firefly was developed together with Perak State Development Corporation and Nimura Genetic Solutions in conformity with the Convention on Biological Diversity.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Luciola sp2 Luciferase cDNA
Depositor OLYMPUS CORPORATION, | Ogoh, Katsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Akiyoshi R, Ogoh K, Suzuki H. 2015. FIREFLY LUCIFERASE, Patent publication US2015/0291938 A1). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14363 pMALuci2Luc DNA solution Please contact us.

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14363_A6Jcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2 RDB14363_A6Jca.seq
>D03707A5_A6Jc_1_Reverse2_B01_04_ABI24.ab1
    1 AGGAATGATT ACGCCAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC GATGAACAAG
   61 AACATCATCT ACGGCCCTCC CCCCGTGTAC CCCCTGGATG ATGGCACAGG CGGCGAGCAG
  121 CTGTACAAGT GCATCCTGAG ATACGCCAAG ATCCCCGAGT GCGTGGCCCT GACCAGCGCC
  181 CACACCAAAG AGAGCATCCT GTACGAGGAA CTGCTGCAGC TGACCTGCAA GCTGGCCCAG
  241 AGCCTGAAGA GATGCGGCAT CACCCGGAAC AGCACAATCG CCGTGTGCAG CGAGAACAAC
  301 CTGCAGTACT TCATCCCCAT CATTGCCGGC CTGTACATCG GAGCCGCCAC AGCCGCCGTG
  361 AACAACCGGT ACAACGAGAG AGAGCTGACC GACATCCTGA ACCTGAGCAA GCCCGACATC
  421 ATCTTTTGCT CCAAAGAGAC ACTGCCCAAG ATCTGCCAGG TCAAGAAGAA GCTGAACTAC
  481 ATCAAAGAAA TCATCGTGCT GGACAGCAAG CACGACAGCG AGCTGGCTCA GTGTCTGGAC
  541 AACTTCATCA GCCACAACTG CAACAAGGAC TTCGACGCCT ACCAGTTCAA GCCCAGCAGC
  601 TTCAACCGGA ACGAACAGGT CGGCCTGATC CTGAACAGCA GCGGCAGCAC CGGCCTGCCC
  661 AAGGGCGTGA TGCTGACCCA CAAGAACCTG GTGGTGCGCT TCAGCCACTG CAAGGACCCC
  721 GTGTTCGGCA ACATCATCAG CCCCGGCACC GCCATCCTGA CCGTGATCCC TTTCCACCAC
  781 GGCTTCGGCA TGTTCACCAC CCTGGGCTAC TTCACCTGTG GCTTCCGGAT CGTGCTGATG
  841 CACACCTTCT ACGAGAAGCT GTTCCTGCAG GCCCTGGAAG ATTACAAGGT GGAAAGCACC
  901 CTGCTGGTGC CTACCCTGAT GACCTTCTTC GCCAAGAGCG CCCTGGTGGA CAAGTACAAC
  961 CTGCCCTACC TGAAAGAGAT CGCCAGCGGC GGAGCCCCCC TGAGCAAGAA TCGGGCGAGC
 1021 CGTGGCCAGA CGGTTTCAAG CTGACCGGCA TCGGCAGGCT ACGACTTGAC GAGACATAGC
1081 GCCGTGCTGA ATCAAC
//
Primer: pUC-M13_-100 RDB14363_A6Jcb.seq
>D03707A5_A6Jc_1_pCU_M13-100_A01_01_ABI24.ab1
    1 GTCTTCAGCG ATTAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC
   61 GGCCAGTGAA TTCTCACAGC TTGCAGTTGG GCTTCTGCAG GATCTGCTTC AGGGCGGTGG
  121 TGTCGATCTT GCCGGTGGAG CCCTTAGGGA TCTCGTCCAG GAACCGCACG CCGCCTCTCA
  181 GCCGCTTCAC GCTGGACACC TGGCTGGACA CGTAGTTGAT CACGTTTTCC TCGGTCAGGT
  241 GTCTGCCGGG TTTCACCACC ACGCAGGCGG CAGGCAGCTC GCCGGCATTA TCGTCGGGGA
  301 TGCCGGTCAC GCCGGCATCC ATGATGCTGG GGTGTGTCAG CAGGATGCCT TCCAGCTCGG
  361 CAGGGGCCAC CTGGTAGCCC TTGTACTTGA TGATGGACTT CAGCCGGTCC ACGATGAAGA
  421 AGTGCTTGTC GTCGTTGTAG TAGCCCAGAT CGCCGCTGTG CAGCCAGCCG TCGTTGTCGA
  481 TGATGGCGTT GGTGGCCTCG ATGTTGTTGC AGTAGCCCTT CATGATCATG TCGCCCTTGA
  541 AGCACAGCTC GCCCACTTCA TTAGGGCCCA GGGCCTTGCC GGTCCGGTGG TCGATGATCT
  601 TGGCGGCGAA GAATGGCACC ACCTTGCCGA TGCTGCCGGG CACTGTCTCG CTCTCGGGGG
  661 TGATCAGCAC GGCGCTGGTT GTCTCGGTCA GGCCGTAGCC CTGCCGGATG GCGTTCAGCT
  721 TGAACCGTCT GGCCACGGCC TCGCCGATTT CTTTGCTCAG GGGGGCTCCG CCGCTGGCGA
  781 TCTCTTTCAG GTAGGGCAGG TTGTACTTGT CCACCAGGGC GCTCTTGGCG AAGAAGGTCA
  841 TCAGGGTAGG CACCAGCAGG GTGCTTTCCA CCTTGTAATC TTCCAGGGCC TGCAGGAACA
  901 GCTTCTCGTA GAAAGGTGTG CATCAGCACG ATCCGGAAGC CACAGGTGAA GTAGCCCAGG
  961 GTGGTGAACA TGCCGAAGCC CGTGGTGGAA AGGGATCACG GGTCAGGATG GGCGGTGCCC
 1021 GGGGCTGATG ATGTGCCCGA ACACGGGGTC CCTGCAGTTG ACTGGAGCGC CACCCAACAG
1081 ATCTGTGGGA TTCAGCATCA CGTCCCTTGG CAGTCGATGG CTGACCGCCG TGTACCC
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Higher intensity luciferase and pH sensor GFP (Japanese text)
Featured content Higher intensity luciferase and pH sensor GFP (English text)

References

original Akiyoshi, R., FIREFLY LUCIFERASE. Patent publication US2015/0291938 A1

2017.08.30

GNP_filter3_RDBDEP_html_170829.pl