Resource data sheet
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pDkumLuc1

Luciferase of Drilaster kumejimensis firefly with higher luminous intensity.

Catalog number RDB14360
Resource name pDkumLuc1
Clone info. Luciferase of Drilaster kumejimensis firefly with higher luminous intensity. A maximum luminous wavelength falls within a range of 564 to 577 nm over the temperature of 25 to 37 centigrade (Fig. 5 of Akiyoshi R, Ogoh K, Suzuki H. 2013. Firefly luciferase. Japan patent unregistered 2013-74861). pDkumLuc1 is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Drilaster kumejimensis Luciferase cDNA
Depositor OLYMPUS CORPORATION, | Ogoh, Katsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Akiyoshi R, Ogoh K, Suzuki H. 2013. Firefly luciferase. Japan patent unregistered 2013-74861). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14360 pDkumLuc1 DNA solution Please contact us.

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14360_A6Jcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2 RDB14360_A6Jca.seq
>D03707A2_A6Jc_3_Reverse2_D04_10_ABI24.ab1
    1 GGGGAAGGGA TTACGGCCAA GCTTGCATGC CTGCAGGTCG ACTCTAGAGG ATCCGATGGA
   61 CATGGAAGAT AAGAACGTGG TGCACGGCCC TGCCAGCTTC TACCCTGTGG ATGATGGCAC
  121 AGCCGGCCAG CAGCTGCACA AGACCATCCT GAGATACGCC CAGCTGCCCG GCACAATCGC
  181 CTTTACCGAT GCCCACACCG AGCAGAACCT GACCTACGCC GAGTACTTCG ACCTGACCTG
  241 CAGACTGGCC GAGAGCATGA AGAGATACGG CCTGGGCCTG AAGCACGCCA TTGCCGTGTG
  301 CAGCGAGAAC AGCCTGAACT TCTTCGCCCC CGTGATCGCC GCCCTGTATC TGGGAGTGGC
  361 CGTGGCCCCC ACCAACGACA TCTACAACGA GAGAGAGCTG TACAACTCCC TGAACATCAG
  421 CCAGCCCACC ATCGTGTTCT GCAGCAAGCG GGCCCTGCTG AAGATCATGG AAGTGCAGAA
  481 AAAGCTGCCC GTGATCCAGA AAATCATCGT GCTGGACCAC AAAGAGGACT TCCAGGGCCT
  541 GCACAGCATG CACAGCTTCA TCAAGCACTA CCTGCCCAGC CACTTCAACG AGCACGACTT
  601 CAAGCCCGAA GAGTTCGACC GGAACGACCA GGTGGCCCTG ATCATGAACA GCAGCGGCAG
  661 CACCGGCCTG CCTAAAGGCG TGATGCTGAC CCACAAGAAT GTGGTCGTGC GGTTCAGCCA
  721 CTGCAAGGAC CCCGTGTTCG GCAACCAGAT CATCCCCGAC ACCGCTATCC TGACCGTGAT
  781 CCCTTTCCAC CACGGCTTCG GCATGTTCAC CACCCTGGGC TACCTGACCT GTGGCTTCCG
  841 GATCGTGGTC ATGCACAAGT TCGAGGAAGA ACTGTTCCTG CGGAGCCTGC AGGACTACAA
  901 GATCCAGAGC GCCCTGCTGG TGCCTACCCT GTTCAGCTTC TTCGCCCAAG AGCACCCTGG
  961 TGGATAAGTA CGACCCTGAG CAACCCTGAA AGAGATCGCC AGCGGGCGGG AGCCCCCCCC
 1021 TGGCCTAAGA GTGGGAGAGT CGGTCGGCCG AGCCGATTAG GCCTGGCCTG GCGCTGCGTC
1081 ACGCTTACGC AACGTGGACA GAGACCACCA GCGGCCCGAT GA
//
Primer: pUC-M13_-100 RDB14360_A6Jcb.seq
>D03707A2_A6Jc_3_pUC-M13_-100_C04_07_ABI24.ab1
    1 GACGGTCAAG GCGATTAAGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
   61 GACGGCCAGT GAATTCTCAC ATCTTGCTCT GGGGCCGCTT CAGGATTTCT TTCAGGCCTC
  121 TGCTGTCGAT CTTGCCGGTC AGGCCCTTAG GGATCTCGTC GATGAAGCGC ACGCCGCCTC
  181 TCAGTTTCTT GCTGCTGGAC ACCTGGCTGG CGACGTAGTC CATCACTTCT TTCTCGGTCA
  241 GGTGCTTGCC CTTTTCCAGC ACCACACAGG CGGCAGGCAG TTCGCCTGCC ACTTCATCAG
  301 GGATGCCGGT CACGCCAGCG TCGAAGATGT AGGGATGCTG CAGCAGCACG CTTTCCAGCT
  361 CGGCAGGGGC CACCTGATAG CCCTTGTACT TGATCAGGGA CTTCAGCCGG TCCACGATGA
  421 AGAAGTGGCC GTCCTCGTCG TAGTAGGCCA GGTCGCCGCT GTGCAGCCAG CCATCCTTGT
  481 CGATCAGGGC GTTGGTGGCC TCGGGGTTGT TGGCGTAGCC CTTCATGATC ATGTCGCCCT
  541 TCAGGCACAG CTCGCCTTTC TGGTGAGGGC CCAGGCTCTG TCTGGTGTCC AGGTCGATGA
  601 TCTTGGCGCT GAAGAATGGC ACCACCTTGC CTGTAGAGCC GGCCTTATCT TCGCCCTCGG
  661 GTGTGATGAT CACGGCGCTG GTTGTCTCTG TCAGTCCGTA GCCCTGCCGC ACGCCAGGCA
  721 GCTTAAACCG CTTGGCGACG GCCTCTCCCA CTTCTTTAGC CAGGGGGGCT CCGCCGCTGG
  781 CGATCTCTTT CACGTTGCTC AGTCGTACTT ATCCACCAGG GTGCTCTTGG CGAAGAAGCT
  841 GAACAGGGTA GGCACCAGCA GGGCGCTCTG GATCTTGTAG TCCTGCAGGC TCCGCAGAAC
  901 AGTTCTTCCT CGAACTTGTG CATGACCACG ATCCGGGAAG CCACAGGTCA GGTAGCCCAG
  961 TGTGGTGAAC ATGCCGAAAG CCGTGGTGGA AAGGATCACG TCAGATAGCG GTGTCGGGGA
 1021 TGATCTGGGT TTGCCGGACA CGGGGTTCCC TTTGCAGTTG TCCTGAACGC AACGAACCAC
1081 ATTTCTTGGG TGGGCTTCAA GCGAT
//
Primer: M13_-40 RDB14360_A6Jcc.seq
>D03707A2_A6Jc_3_M13_-40_D01_04_ABI08.ab1
    1 CAGGCAAGTG AATCTCACAT CTTGCTCTGG GGCCGCTTCA GGATTTCTTT CAGGCCTCTG
   61 CTGTCGATCT TGCCGGTCAG GCCCTTAGGG ATCTCGTCGA TGAAGCGCAC GCCGCCTCTC
  121 AGTTTCTTGC TGCTGGACAC CTGGCTGGCG ACGTAGTCCA TCACTTCTTT CTCGGTCAGG
  181 TGCTTGCCCT TTTCCAGCAC CACACAGGCG GCAGGCAGTT CGCCTGCCAC TTCATCAGGG
  241 ATGCCGGTCA CGCCAGCGTC GAAGATGTAG GGATGCTGCA GCAGCACGCT TTCCAGCTCG
  301 GCAGGGGCCA CCTGATAGCC CTTGTACTTG ATCAGGGACT TCAGCCGGTC CACGATGAAG
  361 AAGTGGCCGT CCTCGTCGTA GTAGGCCAGG TCGCCGCTGT GCAGCCAGCC ATCCTTGTCG
  421 ATCAGGGCGT TGGTGGCCTC GGGGTTGTTG GCGTAGCCCT TCATGATCAT GTCGCCCTTC
  481 AGGCACAGCT CGCCTTTCTG GTGAGGGCCC AGGCTCTGTC TGGTGTCCAG GTCGATGATC
  541 TTGGCGCTGA AGAATGGCAC CACCTTGCCT GTAGAGCCGG CCTTATCTTC GCCCTCGGGT
  601 GTGATGATCA CGGCGCTGGT TGTCTCTGTC AGTCCGTAGC CCTGCCGCAC GCCAGGCAGC
  661 TTAAACCGCT TGGCGACGGC CTCTCCCACT TCTTTAGCCA GGGGGGCTCC GCCGCTGGCG
  721 ATCTCTTTCA GGTTGCTCAG GTCGTACTTA TCCACCAGGG TGCTCTTGGC GAAGAAGCTG
  781 AACAGGGTAG GCACCAGCAG GGCGCTCTGG ATCTTGTAGT CCTGCAGCTC CGCAGGAACA
  841 GTTCTTCCTC GAACTTGTGC ATGACCACGA TCCGGAAGCC ACAGGTCAGG TAGCCCAGGG
  901 TGGTGAACAT GCCGAAGCCG TGGTGGAAAG GGATCACGGT CAGGATAGCG GTGTCGGGGA
  961 TGATCTGGGT TGCCGAACAC GGGGTCCTTT GCAGTGGCTG AACCGCACGA CCACATTCTT
 1021 GTGGGTCAGC ATCACGGCCT TTTAGGCAGG CCGGTGCTGC CGCTGCTGTT CATGATCAGG
 1081 CCACTTGATC GTCGTTCGAC TCATCTGCTT GAGTCCTGCT CGATGAAGTT GACTTGGGCA
1141 GGTAGTGCTT GGAATTGAAA GCT
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Higher intensity luciferase and pH sensor GFP (Japanese text)
Featured content Higher intensity luciferase and pH sensor GFP (English text)

References

original Akiyoshi, R., Firefly luciferase. Japan patent unregistered 2013-74861

2017.08.30

GNP_filter3_RDBDEP_html_170829.pl