Resource data sheet
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pPmatLuc1

Luciferase of Pyrocoelia matsumurai firefly with higher luminous intensity.

Catalog number RDB14359
Resource name pPmatLuc1
Clone info. Luciferase of Pyrocoelia matsumurai firefly with higher luminous intensity. pPmatLuc1 is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Pyrocoelia matsumurai Luciferase cDNA
Depositor OLYMPUS CORPORATION, | Ogoh, Katsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Akiyoshi R, Ogoh K, Suzuki H. 2012. Firefly luciferase. Japan patent unregistered 2012-235756). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14359 pPmatLuc1 DNA solution Please contact us.

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14359_A6Jcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2 RDB14359_A6Jca.seq
>D03707A1_A6Jc_2_Reverse2_B04_04_ABI24.ab1
    1 CATCATCATT ACGCCAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC ACCATGGAAG
   61 ATGACCACAA GAACATCGTG CACGGCCCTG CCCCATTCTA CCCCCTGGAA GAGGGAACAG
  121 CCGGCGAGCA GCTGCACCGG GCCATGAAGA GATATGCCCA GGTGCCCGGC ACAATCGCCT
  181 TCACCGATGC CCACGTGGAA GTGAACATCA CCTACAGCGA GTACTTCGAG ATGGCCTGCC
  241 GGCTGGCCGA GACAATGAAG CGCTATGGCC TGGGCCTGCA GCACCACATT GCCGTGTGCA
  301 GCGAGAACAG CCTGCAGTTC TTCATGCCCG TGTGTGGCGC CCTGTTCATC GGAGTGGGAG
  361 TGGCCCCCAC CAACGACATC TACAACGAGA GAGAGCTGTA CAACAGCCTG AGCATCAGCC
  421 AGCCCACCAT CGTGTTCTGC AGCAAGCGGG CCCTGCAGAA AATCCTGGGC GTGCAGAAAA
  481 AGCTGCCCGT GATCGAGAAG ATCGTGATCC TGGACAGCCG CGAGGACTAC ATGGGCAAGC
  541 AGAGCATGTA CAGCTTCATC GAGAGCCATC TGCCCGCTGG CTTCAACGAG TACGACTACG
  601 TGCCCGACAC CTTCGACAGA GAGACAGCCA CCGCCCTGAT CATGAACAGC AGCGGCTCTA
  661 CCGGCCTGCC CAAGGGCGTG GAACTGACCC ACAAGAATGT GTGCGTGCGG TTCAGCCACT
  721 GCCGGGACCC TGTGTTCGGC AACCAGATCA TCCCCGACAC CGCTATCCTG ACCGTGATCC
  781 CCTTCCACCA CGGCTTCGGC ATGTTCACCA CCCTGGGCTA CCTGACCTGC GGCTTCCGGA
  841 TCGTGCTGAT GTACAGATTC GAGGAAGAAC TGTTCCTGCG GAGCCTGCAG GACTACAAGA
  901 TCCAGAGCGC CCTGCTGGTG CCTACCCTGT TCAGCTTCTT CGCCAAGAGC ACCCTGGTGG
  961 ATAAGTACGA CCTGAGCAAC CCTGCACGAG ATCGCCTCTG GGCGGAGCCC CCCCCTGGCT
 1021 AAGGAAGGTG GGAGAGCCCG TTGGTCAGCC GGATTCAAGC TGCCGTGGCA TCAGAACAGG
1081 ACTTACGTCT TGACCGAAGC ACAACCCGTC G
//
Primer: pUC-M13_-100 RDB14359_A6Jcb.seq
>D03707A1_A6Jc_2_pUC-M13_-100_A04_01_ABI24.ab1
    1 GGCCTGTCAG GCCGATTAGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC
   61 GACGGCCAGT GAATTCTCAC AGCTTGCTTT TCTTGCCCAT TGTCAGCATC TCGCGGATCT
  121 TCCGGCTGTC GATCTTGCCT GTCAGGCCCT TTGGCACCTC GTCCACGAAC TTCACGCCGC
  181 CTCTCAGCCT CTTGCTGGCG GTCACTTGTC CGGCCACGTA GTCCATCACT TCCTGCTCGG
  241 TCATGGTCTT GCCCTCTTCC AGCACCACCA CAGCGGCAGG CAGTTCGCCA GCATCAGCAT
  301 CAGGGATGCC GGCCACCCCG GCATCGAAGA TGAAGGGATG CTGCAGCAGG ATAGATTCCA
  361 GCTCGGCGGG TGGCACCTGG TAGCCCTGGT ACTTGATCAG GGACTTCAGC CGGTCCACGA
  421 TGAAGAAGTG GCCGTCCTTG TCGTAGTAGG CCAGGTCGCC AGAGTGCAGC CAGCCATCCT
  481 TGTCGATCAG GGCATTGGTG GCCTCGGGGT TGTTCACGTA GCCCTTCATA ATCATGGGGC
  541 CCTTCAGACA CAGCTCGCCC CTCTGGTTCA CGCCCAGTGT CTTGCTGGTG TCCAGGTCCA
  601 CAATCTTGGC GCTGAAAAAT GGGGCCACCT TTCCACAGGC GCCAGGCTTA TCGTCGCCCC
  661 TGGGGGTGAT GATCACGGCA GAGGTTGTCT CGGTCAGGCC GTAGCCCTGT CTGATGCCAG
  721 GCAGCTTGAA CCGCTTGGCC ACGGCCTCTC CCACTTCTTT AGCCAGGGGG GCTCCGCCAG
  781 AGGCGATCTC GTGCAGGTTG CTCAGGTCGT ACTTATCCAC CAGGGTGCTC TTGGCGAAGA
  841 AGCTGAACAG GGTAGGCACC AGCAGGGCGC TCTGGATCTT GTAGTCCTGC AGGCTCCGCA
  901 GGAACAGTTC TTCCTCGAAT CTGTACATCA GCACGATCCG GAAGCCGCAG TCAAGGTAGC
  961 CCAGGTGGTG AACATGCCGA AGCCGTGGGT GGAATGGGGA TCACGGGTCC AGGAATAGCG
 1021 GTGTCGGGGA TGATCTGGGA TTGCCGACAC ACGGTCCCGG CAGTGACCTG ATCGCACGCC
1081 ACAACATTCT TTGTTGGGTT CAAGT
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Higher intensity luciferase and pH sensor GFP (Japanese text)
Featured content Higher intensity luciferase and pH sensor GFP (English text)

References

original Akiyoshi, R., Firefly luciferase. Japan patent unregistered 2012-235756

2017.08.30

GNP_filter3_RDBDEP_html_170829.pl