Resource data sheet
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pcDNA3.1(+)-Histac-H3K9/K14

For the detection of acetylation at the 9th and 14th lysine residues of histone H3 by the BRD4 bromodomain.

Catalog number RDB14340
Resource name pcDNA3.1(+)-Histac-H3K9/K14
Clone info. Expression vector of Histac-H3K9/K14, a fluorescent probe to visualize acetylation of histone H3 Lys9 (H3K9) and histone H3 Lys14 (H3K14) in living cells by means of fluorescence resonance energy transfer (FRET), under the control of CMV promotor. See Fig. 1A of Nakaoka, S. et. al., ACS Chem. Biol. 11 (3): 729-733, 2016.
Expression was confirmed by observation fluorescent in COS7 cells under the microscope by the depositor.
[Data sheet: http://dna.brc.riken.jp/DataSheet/RDB14340]
Vector backbone pcDNA3.1
Size of vector backbone 5.4 kb
Classification Plasmid
Type_of_insert fragment Human cDNA ()
Deposotor's page Sasaki, Kazuki |
Reference Jump to table (bottom).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakaoka, S. et al., ACS Chem. Biol., 11 (3), 729-733, 2006)
(In Japanese [open/close])
Remarks ((Additional form)) GFP-A
(In Japanese [open/close])
Fee (non-profit org.)

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Featured content Histac for visualization of acetylation of the Histon (in English)
Featured content Epigenetics clone (in English)
original - reference Nakaoka, S., A Genetically Encoded FRET Probe to Detect Intranucleosomal Histone H3K9 or H3K14 Acetylation Using BRD4, a BET Family Member. ACS Chem. Biol. 11 (3): 729-733 (2016). PMID 25946208.

2016.09.27

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