Resource data sheet
Prev.

pcDNA3.1(+)-Histac-H3K9/K14

For the detection of acetylation at the 9th and 14th lysine residues of histone H3 by the BRD4 bromodomain.

Catalog number RDB14340
Resource name pcDNA3.1(+)-Histac-H3K9/K14
Clone info. Expression vector of Histac-H3K9/K14, a fluorescent probe to visualize acetylation of histone H3 Lys9 (H3K9) and histone H3 Lys14 (H3K14) in living cells by means of fluorescence resonance energy transfer (FRET), under the control of CMV promotor. See Fig. 1A of Nakaoka, S. et. al., ACS Chem. Biol. 11 (3): 729-733, 2016.
Expression was confirmed by observation fluorescent in COS7 cells under the microscope by the depositor.
Vector backbone pcDNA3.1 (Plasmid)
Size of vector backbone 5.4 kb
Gene/insert name Human BRD4 cDNA Mouse Histone 3/n cDNA
Depositor Sasaki, Kazuki |
 
Other clones in our bank human BRD4 (NCBI Gene 23476) | mouse Hist1h3a (NCBI Gene 360198) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakaoka, S. et al., ACS Chem. Biol., 11 (3), 729-733, 2006)
(Japanese text [open/close])
Remarks ((Additional form)) GFP-A
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14340 pcDNA3.1(+)-Histac-H3K9/K14 DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14340_A6Icp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14340_A6Ica.seq
>D03775A1_A6Ic_1_CMV-Forward_F08_06_ABI08.ab1
    1 ACGAAAACTC CGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
  121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GCGTTTAAAC TTAAGCTTGG TACCATGGTG
  181 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
  241 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
  301 CTGACCCTGA AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
  361 ACCACCCTGG GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC
  421 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
  481 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
  541 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
  601 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCACCGCCG ACAAGCAGAA GAACGGCATC
  661 AAGGCCAACT TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT CGCCGACCAC
  721 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
  781 AGCTACCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG
  841 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GGAATTCTCC
  901 AACCCTAACA AGCCCAAGAG GCAGACCAAC CAACTGCAAT ACCTGCTCAG AGTGGTGCTC
  961 AGACACTATG GGAAACACCA GTTTGCATGG CCTTTCCAGC AGCCTGTGAT GCCGTCAGCT
 1021 GACCTCCCTG ATTACTATAG ATCATTAAAA CGCCTATGGA TATGGCACAT AAGAGCGCTG
 1081 CAAAACACCT AATAACTTGC ATGCTCAAGA TGTTATCAGA CTTCACCTAT GGTTACAATG
1141 GTACATTCTA CACAGCCCTG GGGAGAGTGA ACCATAGT
//
Primer: attB2-R1 RDB14340_A6Icb.seq
>D03775A1_A6Ic_1_attB2-R1_G07_07_ABI08.ab1
    1 GACTTCGCGG ATGCGACGGG CCAGTTGGAT GTCCTTGGGC ATGATGGTGA CACGCTTGGC
   61 GTGGATGGCG CACAGGTTGG TGTCCTCAAA CAGACCCACA AGGTAGGCCT CACAGGCCTC
  121 CTGCAGAGCC ATGACTGCCG AGCTCTGGAA GCGCAGGTCG GTCTTGAAGT CCTGCGCGAT
  181 CTCGCGCACC AGGCGCTGGA ACGGCAGCTT GCGGATCAGC AGCTCGGTCG ACTTCTGGTA
  241 ACGCCGGATC TCGCGCAGCG CCACGGTGCC GGGACGGTAG CGGTGAGGCT TCTTCACGCC
  301 GCCGGTAGCC GGGGCGCTCT TGCGGGCGGC CTTGGTGGCC AGCTGCTTAC GCGGGGCCTT
  361 GCCACCGGTG GACTTGCGAG CGGTCTGCTT AGTACGAGCC ATGGATCCAG AACCTCCTCC
  421 ACCAGATCCA CCTCCTCCGT CGACCACCAC TGGCTCCTCA GGCTCGTCCG GCATCTTGGC
  481 AAAGCGCATT TCGAACACAT CCTGGAGCTT GCGGGCCATG GCCACCACCT CATGGTCAGG
  541 AGGGTTGTAC TTATAGCAGT TGGAGAACAT CAATCGGACG TCAGCACCAA ACTCCTGAGC
  601 ATCACGGTAC TCACGGGCCT CCAGTTTAGA CTTGATTGTG CTCATGTCCA TGGGGTGCTT
  661 GATGATGTCA CAGTAGTCGT GTAGGCCCAG TGCCTCCACG TCCACAGGCT TGTAGAAGGG
  721 CCAGGCGTAG CGGCGTGCTT CTTGGCAAAC ATCTCCTTGA GGATGCCGCT GCAGCACTTG
  781 AGCTGCTCCG AGACCTTGCT GCTCTTCTCT GGTGCTGGGT GCTGCTGAGA GTCGGGCACG
  841 TCCTTCTTTG GAGGTTTCAC AGGCCGGCTG CTCTCCCGCC GCTGGCCCAG CTTGGTGGTC
  901 TTGGGCTCCG GGGGCAGCGA GGGTGGCTCG TGAATGGGGT CAATGGTGGT GGGGGTGGTG
  961 GTGTCTGCTT TCTCTTTCAC CTCCCTTCTT TGTCTTCACA GGCTGTGGGT GGCCGCGATG
 1021 ATGGGATGGG TGCTCTGTAC AGGCTGGGAG CCTGGACGGC TGGGGGTTGT GGCTGAGGGC
1081 ACTTGACCGG GGCGTCTTGC CATGACCGTG GGGGAGGCAC
//
Primer: BGH_rev2 RDB14340_A6Icc.seq
>D03775A1_A6Ic_1_BGH_rev2_G08_07_ABI08.ab1
    1 CTCTAGGGTC AGATGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC
   61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTCTAGAC TCGAGTTACT TGTACAGCTC
  121 GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG
  181 CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG
  241 CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT
  301 GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT TCTGCTTGTC
  361 GGCGGTGATA TAGACGTTGT GGCTGATGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT
  421 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC
  481 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC
  541 CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG
  601 GTAGCGGCTG AAGCACTGCA CGCCCCAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC
  661 GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC
  721 CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG
  781 GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGATAT CAACCACTTT
  841 GTACAAGAAA GCTGGGTCGA CAGCCCTCTC CCCGCGGATG CGACGGGCCA GTTGGATGTC
  901 CTTGGGCATG ATGGTGACAC GCTTGGCGTG GATGGCGCAC AGGTTGGTGT CCTCAAACAG
  961 ACCACAAGTA GGCCTCACAG GCCTCCTGCA GAGCCATGAC TGCCGAGCTC TGGAAGCGCA
 1021 GTCGGTCTTG AAGTCTGCGC GATCTCGCGC ACAGGCGCTG GAACGGCAGC TGCGATCAGC
 1081 AGCTCGTCGA CCTCTGGTAC GCGATCTCGC GCAGCGCACG TGCCGGACGG TAGCGGTGAA
1141 GCTCCTCAGC GGCGGTTAGC TGGGGCCGCC CTCTTTGGCC GGGGCGAGCC CCC
//
Primer: SV40pro_F RDB14340_A6Icd.seq
>D03775A1_A6Ic_1_SV40pro-F_H06_08_ABI08.ab1
    1 CCCTGTTCCC TGGGCATTCC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
   61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA
  121 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
  181 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
  241 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
  301 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
  361 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
  421 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
  481 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
  541 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
  601 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC
  661 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
  721 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
  781 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
  841 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
  901 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA
  961 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT
 1021 TCGGAATCGT TTTCCGGGAC GCCGCTGGGA TGATCCTCCA GCGCGGGATC TCATGCTGGA
 1081 GTTCTTCGCC CACCCACTGT TTATTGCAGC TTATATGGTT ACGATAAGGC CATTAGCATT
1141 CACAAGTTCA CAAAATAAAG CCAATTTTTT TTTGCACG
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Histac for visualization of acetylation of the Histon (English text)
Featured content Epigenetics clone (English text)

References

original Nakaoka, S., A Genetically Encoded FRET Probe to Detect Intranucleosomal Histone H3K9 or H3K14 Acetylation Using BRD4, a BET Family Member. ACS Chem. Biol. 11 (3): 729-733 (2016). PMID 25946208.

2017.09.15

GNP_filter3_RDBDEP_html_170903.pl